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Live vaccine, live vaccine freeze-dried powder, live vaccine protective agent as well as preparation method and application thereof

A technology of live vaccine and protective agent, applied in vaccines, freeze-dried delivery, veterinary vaccines, etc., can solve the problems of immunization failure, difficulty in storage, transportation and use, and failure to protect animals.

Pending Publication Date: 2020-09-25
GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improper storage methods can lead to a decrease in the bacterial survival rate of the vaccine, or even the death of most bacteria or the endotoxin produced by disintegration, which is one of the factors that cause allergic reactions; in addition, low temperature storage brings difficulties to storage, transportation and use in remote areas
Improper storage of the vaccine can cause animal allergies and cause death, resulting in immune failure and failing to achieve the effect of protecting animals

Method used

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  • Live vaccine, live vaccine freeze-dried powder, live vaccine protective agent as well as preparation method and application thereof
  • Live vaccine, live vaccine freeze-dried powder, live vaccine protective agent as well as preparation method and application thereof
  • Live vaccine, live vaccine freeze-dried powder, live vaccine protective agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] The present invention provides a preparation method of the live vaccine protective agent described in the above technical scheme, comprising the following steps:

[0036] Mixing water-soluble gelatin, oligosaccharides, sorbitol, polyvinylpyrrolidone and the first remaining water for injection for high-temperature sterilization to obtain the first raw material;

[0037] After mixing and filtering L-sodium glutamate, sodium ascorbate, 199 medium and the second remaining water for injection, the second raw material is obtained;

[0038] After the first raw material is mixed with the second raw material, the insufficient part is made up by the third water for injection to obtain a live vaccine protective agent.

[0039] The present invention mixes water-soluble gelatin, oligosaccharide, sorbitol, polyvinylpyrrolidone K30 and the first remaining water for injection for high-temperature sterilization to obtain the first raw material. In the present invention, the amount of t...

Embodiment 1

[0061] Raw material preparation: 1.5g water-soluble gelatin, 5g sucrose, 5g sorbitol, 0.1g polyvinylpyrrolidone K30, 5g L-sodium glutamate, 1.5g sodium ascorbate, 0.5g 199 culture medium, and make up the balance to 100mL with distilled water .

[0062] Mix 1.5g of water-soluble gelatin, 5g of sucrose, 5g of sorbitol, 0.1g of polyvinylpyrrolidone K30 and 30mL of water for injection, and sterilize at 25°C for 25 minutes to obtain the first raw material;

[0063] After mixing and filtering 5 g of L-sodium glutamate, 1.5 g of sodium ascorbate, 0.5 g of 199 medium and 30 mL of water for injection, the second raw material was obtained;

[0064]After mixing the first raw material and the second raw material, the insufficient part is made up with sterilized water for injection to obtain the live vaccine protective agent.

Embodiment 2

[0066] Preparation of Bacterial Solution for Yak Paratyphoid Live Vaccine

[0067] The seed solution was prepared according to the requirements of "Regulations of Veterinary Biological Products of the People's Republic of China" (2000 edition, Ministry of Agriculture of the People's Republic of China.-Beijing: Chemical Industry Press, 2000, hereinafter referred to as "Regulations").

[0068] Inoculate the seed solution at 2% into 5 bottles of sterilized 200mL nutrient broth medium, place it at 37°C for 185r / min to ferment and cultivate for 17h, and centrifuge the bacterial solution at 3500r / min for 20min to collect all the sludge and use Suspended in sterilized physiological saline, mixed thoroughly, and used as a bacterial solution for seedling production.

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Abstract

The invention belongs to the technical field of live vaccine protective agents, and particularly relates to a live vaccine, a live vaccine freeze-dried powder, a live vaccine protective agent as wellas a preparation method and application thereof. The live vaccine protective agent comprises water-soluble gelatin, oligosaccharides, sorbitol, polyvinylpyrrolidone, sodium ascorbate, sodium L-glutamate, a 199 culture medium and water for injection. The live vaccine protectant provided by the invention can increase the survival rate and shelf life of bacteria, and the protective function of the live vaccine protective agent on bacteria under a high temperature condition can be improved; and the situation of animal allergies and immune failure caused by improper storage of the live vaccine canbe also avoided.

Description

technical field [0001] The invention belongs to the technical field of live vaccine protective agents, and in particular relates to a live vaccine, a live vaccine freeze-dried powder, a live vaccine protective agent and a preparation method and application thereof. Background technique [0002] The thermal sensitivity of active organisms such as bacteria and their related products has always been a technical bottleneck for long-term storage of live vaccines. my country has a vast territory, large climate differences, and uneven economic levels. The phenomenon of short-term exposure to high temperature environments (even above 40°C) still exists during vaccine transportation and use. Therefore, higher requirements are put forward for the heat resistance of live vaccines. [0003] At present, in the vaccine preparation process, the traditional freeze-drying protective agents in bacterial live vaccines are mainly sucrose and gelatin protective agents. ), with a short shelf lif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K47/42A61K47/32A61K47/26A61K47/22A61K47/18A61K39/112A61P31/04
CPCA61K9/19A61K39/0275A61K47/183A61K47/22A61K47/26A61K47/32A61K47/42A61K2039/522A61K2039/552A61P31/04Y02A50/30
Inventor 陈伯祥李元新赵子惠成伟伟杨明
Owner GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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