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Heat-resistant protecting agent, room-temperature-preserved swine fever live vaccine and preparation method and application of room-temperature-preserved swine fever live vaccine

A heat-resistant protective agent and live vaccine technology, which is applied in biochemical equipment and methods, vaccines, veterinary vaccines, etc., to achieve the effect of reasonable matching, low loss and maintaining activity

Active Publication Date: 2017-10-10
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many researchers have been studying and improving heat-resistant processes, such as spray drying, biomimetic mineralization, etc., but they are all at the level of laboratory research

Method used

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  • Heat-resistant protecting agent, room-temperature-preserved swine fever live vaccine and preparation method and application of room-temperature-preserved swine fever live vaccine
  • Heat-resistant protecting agent, room-temperature-preserved swine fever live vaccine and preparation method and application of room-temperature-preserved swine fever live vaccine
  • Heat-resistant protecting agent, room-temperature-preserved swine fever live vaccine and preparation method and application of room-temperature-preserved swine fever live vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 heat-resistant protective agent 1 and swine fever live vaccine 1

[0035] Unless otherwise specified, the percentages in this embodiment are percentages by weight.

[0036] Heat-resistant protectant 1 recipe:

[0037]

[0038] The specific preparation steps of heat-resistant protective agent 1 are as follows: dissolve raffinose, maltose, sucrose, lactose, glucose, and polysorbate 80 in water, and sterilize by high-pressure steam sterilization (110° C. for 20 min); Diol 8000, tyrosine, and silk fibroin are dissolved in water, and sterilized by 0.22 μm filtration; the autoclaved part and the filtered sterilized part are mixed in equal volumes, and water for injection is supplemented to obtain the heat-resistant protective agent 1 .

[0039] Preparation of swine fever live vaccine 1: heat-resistant protective agent 1 and swine fever virus liquid (C strain, virus content 50 × 10 4 RID / mL) was mixed at a volume ratio of 3:1, distributed ...

Embodiment 2

[0041] The preparation of embodiment 2 heat-resistant protective agent 2 and swine fever live vaccine 2

[0042] Unless otherwise specified, the percentages in this embodiment are percentages by weight.

[0043] Heat Resistant Protectant 2 Recipe:

[0044]

[0045] The specific preparation steps of the heat-resistant protective agent 2 are as follows: dissolve raffinose, maltose, sucrose, lactose, glucose, polysorbate 80 in water, and sterilize by high-pressure steam sterilization; polyethylene glycol 8000, phenolic acid Amino acid and silk fibroin are dissolved in water, and are sterilized by filtration; equal volumes of the autoclaved part and the filtered sterilized part are mixed, and water for injection is supplemented to obtain the heat-resistant protective agent 2.

[0046] Preparation of swine fever live vaccine 2: heat-resistant protective agent 2 and swine fever virus liquid (C strain, virus content 50 × 10 4 RID / mL) was mixed at a volume ratio of 1:1, divided int...

Embodiment 3

[0048] The preparation of embodiment 3 heat-resistant protective agent 3 and swine fever live vaccine 3

[0049] Unless otherwise specified, the percentages in this embodiment are percentages by weight.

[0050] Heat Resistant Protectant 3 Recipe:

[0051]

[0052] The specific preparation steps of the heat-resistant protective agent 3 are as follows: dissolve raffinose, maltose, sucrose, lactose, glucose, polysorbate 80 in water, and sterilize by high-pressure steam sterilization; polyethylene glycol 8000, phenolic acid Amino acid and silk fibroin are dissolved in water, and are sterilized by filtration; equal volumes of the autoclaved part and the filtered sterilized part are mixed, and water for injection is supplemented to obtain the heat-resistant protective agent 3.

[0053] Preparation of swine fever live vaccine 3: heat-resistant protective agent 3 and swine fever virus liquid (C strain, virus content 50 × 10 4 RID / mL) was mixed at a volume ratio of 3:1, subpackag...

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Abstract

The invention discloses a heat-resistant protecting agent. The heat-resistant protecting agent contains the following components in percentage by mass: 1%-5% of raffinose, 5%-10% of maltose, 15%-30% of saccharose, 1%-5% of lactose, 1%-5% of glucose, 0.1%-1.5% of polysorbate 80, 0.1%-0.5% of polyethylene glycol 8000, 0.5%-3% of tyrosine, 3%-6% of silk fibroin and the balance of injection water. The invention further discloses a preparation method of the heat-resistant protecting agent. The invention further discloses a room-temperature-preserved swine fever live vaccine and a preparation method thereof. The preparation method comprises the steps of mixing the heat-resistant protecting agent with swine fever live virus liquid, and carrying out gradient vacuum drying, so as to obtain the swine fever live vaccine. The prepared swine fever live vaccine has a dense dry foam appearance and presents a glass sample slice layer structure under a scanning electron microscope, the glass-transition temperature reaches up to 50 DEG C and is 30 DEG C higher than that of a conventional freeze-dried vaccine, and the prepared swine fever live vaccine can be preserved at 37 DEG C for 3 months and 25 DEG C for one year.

Description

technical field [0001] The invention belongs to the field of vaccine production and storage, and in particular relates to a heat-resistant protective agent, a live vaccine for swine fever that can be stored at room temperature and a preparation method and application thereof, in particular to a live vaccine for swine fever that can be stored at room temperature and a preparation method thereof. Background technique [0002] Classical swine fever (CSF) is an acute, febrile and highly contagious infectious disease caused by classical swine fever virus (Classical Swine Fever Virus, CSFV) of the genus Pestivirus in the family Flaviviridae. High, causing huge safety hazards and economic losses to the pig industry. Currently on the market to prevent classical swine fever is the live cell vaccine of classical swine fever and the spleen lymphocytic vaccine of adult rabbit tissue, both of which are based on the C strain. [0003] The classical swine fever virus is very sensitive to ...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/26A61K47/10A61K47/18A61K39/187A61P31/14
CPCA61K39/12A61K47/10A61K47/183A61K47/26A61K47/42A61K2039/5254A61K2039/552C12N2770/24334A61K9/0019A61P31/14A61K9/08A61K2039/525
Inventor 吕芳卢宇侯继波赵艳红邓碧华张金秋张晓燕左晓昕
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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