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Heat-resistant protective agent, swine fever live vaccine that can be stored at room temperature, preparation method and application thereof

A technology of heat-resistant protective agent and swine fever at room temperature, which is applied in the direction of biochemical equipment and methods, vaccines, veterinary vaccines, etc., to achieve the effect of reasonable matching, low loss and maintaining activity

Active Publication Date: 2020-02-07
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many researchers have been studying and improving heat-resistant processes, such as spray drying, biomimetic mineralization, etc., but they are all at the level of laboratory research

Method used

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  • Heat-resistant protective agent, swine fever live vaccine that can be stored at room temperature, preparation method and application thereof
  • Heat-resistant protective agent, swine fever live vaccine that can be stored at room temperature, preparation method and application thereof
  • Heat-resistant protective agent, swine fever live vaccine that can be stored at room temperature, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 heat-resistant protective agent 1 and swine fever live vaccine 1

[0035] Unless otherwise specified, the percentages in this embodiment are percentages by weight.

[0036] Heat-resistant protectant 1 recipe:

[0037]

[0038] The specific preparation steps of heat-resistant protective agent 1 are as follows: dissolve raffinose, maltose, sucrose, lactose, glucose, and polysorbate 80 in water, and sterilize by high-pressure steam sterilization (110° C. for 20 min); Diol 8000, tyrosine, and silk fibroin are dissolved in water, and sterilized by 0.22 μm filtration; the autoclaved part and the filtered sterilized part are mixed in equal volumes, and water for injection is supplemented to obtain the heat-resistant protective agent 1 .

[0039] Preparation of swine fever live vaccine 1: heat-resistant protective agent 1 and swine fever virus liquid (C strain, virus content 50 × 10 4 RID / mL) was mixed at a volume ratio of 3:1, distributed ...

Embodiment 2

[0041] The preparation of embodiment 2 heat-resistant protective agent 2 and swine fever live vaccine 2

[0042] Unless otherwise specified, the percentages in this embodiment are percentages by weight.

[0043] Heat Resistant Protectant 2 Recipe:

[0044]

[0045] The specific preparation steps of the heat-resistant protective agent 2 are as follows: dissolve raffinose, maltose, sucrose, lactose, glucose, polysorbate 80 in water, and sterilize by high-pressure steam sterilization; polyethylene glycol 8000, phenolic acid Amino acid and silk fibroin are dissolved in water, and are sterilized by filtration; equal volumes of the autoclaved part and the filtered sterilized part are mixed, and water for injection is supplemented to obtain the heat-resistant protective agent 2.

[0046] Preparation of swine fever live vaccine 2: heat-resistant protective agent 2 and swine fever virus liquid (C strain, virus content 50 × 10 4 RID / mL) was mixed at a volume ratio of 1:1, divided int...

Embodiment 3

[0048] The preparation of embodiment 3 heat-resistant protective agent 3 and swine fever live vaccine 3

[0049] Unless otherwise specified, the percentages in this embodiment are percentages by weight.

[0050] Heat Resistant Protectant 3 Recipe:

[0051]

[0052] The specific preparation steps of the heat-resistant protective agent 3 are as follows: dissolve raffinose, maltose, sucrose, lactose, glucose, polysorbate 80 in water, and sterilize by high-pressure steam sterilization; polyethylene glycol 8000, phenolic acid Amino acid and silk fibroin are dissolved in water, and are sterilized by filtration; equal volumes of the autoclaved part and the filtered sterilized part are mixed, and water for injection is supplemented to obtain the heat-resistant protective agent 3.

[0053] Preparation of swine fever live vaccine 3: heat-resistant protective agent 3 and swine fever virus liquid (C strain, virus content 50 × 10 4 RID / mL) was mixed at a volume ratio of 3:1, subpackag...

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Abstract

The invention discloses a heat-resistant protective agent, which comprises 1% to 5% of raffinose, 5% to 10% of maltose, 15% to 30% of sucrose, 1% to 5% of lactose and 1% to 5% of glucose according to the mass percentage. %, polysorbate 80 0.1% to 1.5%, polyethylene glycol 8000 0.1% to 0.5%, tyrosine 0.5% to 3%, silk fibroin 3% to 6%, and the rest is water for injection. The invention also discloses a preparation method of the heat-resistant protective agent. The invention also discloses a live vaccine for swine fever that can be stored at room temperature and a preparation method thereof. After mixing the heat-resistant protective agent and the live swine fever virus liquid, the live swine fever vaccine is obtained through gradient vacuum drying. The appearance of the vaccine prepared by the present invention is dense dry foam, showing a glass-like sheet structure under a scanning electron microscope, and its glass transition temperature is as high as 50°C, which is 30°C higher than that of conventional freeze-dried vaccines, and can be stored at 37°C for 3 months , stored at 25°C for one year.

Description

technical field [0001] The invention belongs to the field of vaccine production and storage, and in particular relates to a heat-resistant protective agent, a live vaccine for swine fever that can be stored at room temperature and a preparation method and application thereof, in particular to a live vaccine for swine fever that can be stored at room temperature and a preparation method thereof. Background technique [0002] Classical swine fever (CSF) is an acute, febrile and highly contagious infectious disease caused by classical swine fever virus (Classical Swine Fever Virus, CSFV) of the genus Pestivirus in the family Flaviviridae. High, causing huge safety hazards and economic losses to the pig industry. Currently on the market to prevent classical swine fever is the live cell vaccine of classical swine fever and the spleen lymphocytic vaccine of adult rabbit tissue, both of which are based on the C strain. [0003] The classical swine fever virus is very sensitive to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/26A61K47/10A61K47/18A61K39/187A61P31/14
CPCA61K39/12A61K47/10A61K47/183A61K47/26A61K47/42A61K2039/5254A61K2039/552C12N2770/24334A61K9/0019A61P31/14A61K9/08A61K2039/525
Inventor 吕芳卢宇侯继波赵艳红邓碧华张金秋张晓燕左晓昕
Owner JIANGSU ACAD OF AGRI SCI
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