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Flight mass spectrum biochip for health risk assessment and its detection method

A flight mass spectrometry and biochip technology, applied in the field of flight mass spectrometry biochips and its detection, can solve problems such as the inability to effectively assess personal health risks, and achieve the effect of avoiding clinical misdiagnosis

Inactive Publication Date: 2013-04-24
上海华翼生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0002] Since the 20th century, life science has achieved great development, but it is still unable to effectively assess the health risks of individuals, so that it is impossible to reveal the level of disease risk in genetics and give personalized health interventions

Method used

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  • Flight mass spectrum biochip for health risk assessment and its detection method
  • Flight mass spectrum biochip for health risk assessment and its detection method
  • Flight mass spectrum biochip for health risk assessment and its detection method

Examples

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Embodiment Construction

[0011] The experimental method that does not indicate specific condition in the following examples, usually according to conventional conditions, such as Sambrook et al., molecular cloning: the conditions described in the laboratory manual (New York: Cold Spring Harbor Laboratory Press, 1989), or according to the manufacturer suggested conditions.

[0012] Step 1 Extract DNA from the subject sample: use Wizard Genomic DNA purification Kit (Promega) or Tissue (MN) extracts DNA from tissues, cell blood samples or oral mucosa, quantifies it with a spectrophotometer, and checks its quality by agarose gel electrophoresis. The DNA that passed the quality inspection was adjusted to a concentration of 50ηg / ml, and stored at -20°C for future use.

[0013] Step 2 Design PCR primers and single-base extension primers: design PCR primers and single-base extension primers for SNP sites (see Table 1).

[0014] Table 1

[0015]

[0016]

[0017]

[0018] Step 3 Perform PCR amplif...

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Abstract

The invention discloses a flight mass spectrum biochip for health risk assessment. The biochip includes a sample analyte and a mass spectrum chip matrix that undergo cocrystallization. The sample analyte comprises a?target?gene, a PCR primer and a single base extension primer used for innate potential assessment. The invention also discloses a detection method making use of the chip. The method consists of: extracting the sample DNA of a detection subject; designing a PCR primer and a single base extension primer; conducting target gene PCR amplification and single base extension on the sample DNA to form a sample analyte; and subjecting the sample analyte and the mass spectrum chip?matrix?to cocrystallization, and detecting the SNP locus of the target gene. The gene chip provided in the invention can reveal the risk of a detection subject in suffering from a disease in the aspect of heredity so as to further provide personalized health intervention measures, thus enabling the detection subject to maintain a good health level.

Description

technical field [0001] The invention relates to a flight mass spectrometry biochip used for health risk assessment and a detection method thereof. Background technique [0002] Since the 20th century, life science has achieved great development, but it is still unable to effectively assess the health risks of individuals, so that it is impossible to reveal the level of disease risk in genetics and give personalized health interventions. Contents of the invention [0003] The invention provides a flight mass spectrometry biochip for health risk assessment and a detection method thereof. [0004] A flying mass spectrometry biochip for evaluating talent potential, comprising a sample analyte and a mass spectrometry chip matrix, the sample analyte is co-crystallized with the mass spectrometry chip matrix, and the sample analyte includes a target gene for talent potential assessment 、PCR引物和单碱基延伸引物,所述目的基因对应的SNP位点包括;rs2273535、rs1866813、rs1056836、rs12304921、rs2285053、rs2165241、rs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张成虎
Owner 上海华翼生物科技有限公司
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