MS4A6A gene application
An MS4A6A and gene technology, applied in the field of MS4A6A gene, can solve the problems of no significant increase in sensitivity, low sensitivity, and long time consumption.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] In this embodiment, the population is divided into three groups: tuberculosis patients, latent infection population and healthy population (20 cases each). By detecting the change of MS4A6A gene mRNA in each peripheral blood mononuclear cell (PBMC), it is found that it is present in tuberculosis patients. A clear trend of up-regulated expression.
[0024] In this example, the quantitative RT-PCR method was used to detect the expression changes of MS4A6A gene in each case. Specific steps are as follows:
[0025] Step 1: Preparation of Peripheral Blood Mononuclear Cell (PBMC) Suspension
[0026] Add 5ml of lymphocyte separation medium (Fresenius Kabi NOrge As: LYS3773) into the centrifuge tube; take 2ml of heparin anticoagulated venous blood from the above-mentioned confirmed tuberculosis patients, patients with latent infection and healthy people and an equivalent amount of 1M phosphate buffer solution (PBS ) to mix well to obtain a mixed solution, use a pipette to sl...
Embodiment 2
[0057] In this embodiment, the crowd is divided into three groups: 9 cases of tuberculosis patients, 6 cases of latently infected people and healthy people, by detecting the change of MS4A6A gene mRNA in each peripheral blood mononuclear cell (PBMC) by gene chip, it is found that it is significantly different in tuberculosis patients. There was a clear trend of up-regulated expression.
[0058] In this embodiment, the difference in gene expression level of MS4A6A gene in TB, LTBI, and HC in tuberculosis samples is detected by gene chip, including the following four steps:
[0059] Step 1: Chip Preparation
[0060] At present, the preparation of chips mainly uses glass or silicon wafers as the carrier, and the target genes are arranged on the carrier in sequence as probes by the spotting method. The target genes can be divided into genomic DNA and cDNA (or artificially synthesized DNA).
[0061] Step 2: Sample Preparation
[0062] The extraction steps of total RNA in the samp...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com