Primer group and kit for detecting avian Borna virus, and usage method thereof

A virus detection and primer set technology, applied in biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, etc., can solve problems such as death, animal septicemia, and inability to move normally.
CN103074445AInactive Publication Date: 2013-05-01INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2013-05-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a primer group for detecting avian Borna virus. The primer group comprises two pairs of primers, inner primers FIP / BIP and outer primers F3 / B3, designed by using an avian Borna virus matrix protein gene as a target gene, and based on a loop-mediated isothermal amplification technology. The invention also discloses a kit for detecting avian Borna virus and a usage method thereof. The technical scheme provided by the invention can efficiently, accurately detect avian Borna virus, can be widely used in the inspection and quarantine of source of such disease, and lays foundation for study on an animal model of pathogenic mechanism of the RNA virus in a host.
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Description

technical field

[0001] The invention relates to a biological detection reagent, in particular to a primer, a kit and a use method for detection of avian Borna virus. Background technique

[0002] Avian Bornavirus (ABV) is a new Bornavirus (BDV) isolated from parrots with Proventricular Dilatation Disease (PDD). BDV can persist in the host's central nervous system, and its entire life cycle is completed in the nucleus of neurons. After virus infection, the gene fuses with the host cell chromosome, and the viral DNA sequence can be detected in the host genome. Poultry is an important natural foci of BDV, and virus strains have been detected in fecal samples collected in bird activity ponds. PDD was first reported in the 1970s, initially primarily in captive parrots in North America. Due to extensive commercial activities, PDD continues to spread among pet birds. It has been reported all over the world that more than 50 species of parrots have been infected. In addition, the...

Claims

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