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Application of recombinant human cell globin in preparation of fatty liver medicament

A technology of fatty liver and globin, applied in the new application field of protein

Inactive Publication Date: 2013-05-08
董文其
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, no literature has reported that human globin has the function of improving or treating fatty liver, especially alcoholic fatty liver

Method used

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  • Application of recombinant human cell globin in preparation of fatty liver medicament
  • Application of recombinant human cell globin in preparation of fatty liver medicament
  • Application of recombinant human cell globin in preparation of fatty liver medicament

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] Below in conjunction with experiment, further illustrate the present invention.

[0016] In the above experiments, CYGB is the abbreviation of cytoglobin, and rhCYGB is the abbreviation of recombinant human cytoglobin.

[0017] Preparation of rhCYGB:

[0018] Induced expression of rhCYGB: the PET28a-rhCYGB-BL21(DE3) strain was used with Kan + The LB culture medium was cultivated overnight, and transferred to fresh LB at a ratio of 1:100 the next day (Kan + ) culture medium, when OD 600 When it reaches 0.6, add IPTG to a final concentration of 1 mmol / L, induce culture at 37°C for 4 h, and harvest the bacteria. Detected by 12% SDS-PAGE, the electrophoresis is as follows figure 1 As shown in the figure, A: low molecular weight protein Marker; B: PET28a-rhCygb-BL21 (DE3) strain expression product; C: negative empty vector strain PET28a-BL21 (DE3) expression product in the expression product of recombinant bacteria with a molecular weight of about A thick band appeare...

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Abstract

The invention discloses an application of a recombinant human cell globin on preparation of a fatty liver medicament. Through building an alcoholic liver rat model, subcutaneous injection is carried out for treatment according to the dose of 3mg / Kg of rhCYGB. An experiment shows that a recombinant human cell globin medicament is non-toxic; and in comparison with a model control group, an rhCYGB treatment group is remarkably improved, fat droplets are obviously reduced, and hepatic cords are levelly arranged. A reduced glutathione treatment group still has obvious fatty degeneration, and liver cells are lightly swollen. The rhCYGB has better efficacy in the treatment of an alcoholic fatty liver than the reduced glutathione. The recombinant human cell globin has the advantages of low toxicity, safety and effectiveness on fatty livers, and especially the alcoholic fatty liver.

Description

technical field [0001] The invention relates to a new application of protein, in particular to a new application of cytoglobin. Background technique [0002] Alcohol drinking is one of the long-standing habits of human civilization. Moderate drinking has no obvious damage to health, and even has a certain preventive effect on coronary heart disease. But alcohol abuse and alcohol dependence affect millions of people worldwide, with many adverse effects on society and human health. According to the World Health Organization report, there are 2 billion alcohol consumption worldwide, about 76.3 million people have been diagnosed as alcohol abuse, the number of people who die from alcohol abuse has reached 3.2% every year, and 4.0% of people are troubled by diseases. At present, in my country, the incidence of liver damage caused by alcohol is increasing year by year. Alcohol has become the second leading cause of liver damage after viral hepatitis, seriously endangering human h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P1/16
Inventor 董文其魏威李珍文健
Owner 董文其
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