Centrifugal tube structure for extracting DNA (deoxyribonucleic acid) by adopting silicon bead method

Abstract

The invention relates to a centrifugal tube structure for extracting DNA (deoxyribonucleic acid) by adopting a silicon bead method, belonging to the technical field of instruments for separating and purifying nucleic acid DNA or RNA (Ribonucleic Acid). The centrifugal tube structure comprises a centrifugal tube with a centrifugal tube cavity, an inner sleeve, and an impurity filtering mechanism, wherein a connecting band is arranged at the upper part of the centrifugal tube, and a plug cover is arranged at the tail end of the connecting band; the inner sleeve is arranged in the centrifugal tube cavity of the centrifugal tube, and a center hole is arranged at the center of the bottom wall of the inner sleeve; the impurity filtering mechanism is matched with the lower part of the inner sleeve and arranged in the centrifugal tube cavity along with the inner sleeve, and the plug cover is matched with an inner sleeve orifice of the inner sleeve. The centrifugal tube structure has the advantages that the loss of water-soluble DNA templates can be avoided; the impurity removal effect is desirable so as to favorably ensure the amplication of the PCR; the advantages of being convenient, fast and effort-saving to operate can be fully shown; and not only can the influence of TES and SLS on the concentration of adsorption liquid be prevented, the adsorption capability of silicon beads to the DNA can be ensured, but also original DNA templates can be retained so as to obviously improve the detection rate of low-copy biological detection materials.

Description

Technical field [0001] The invention belongs to the technical field of equipment for separating and purifying nucleic acid DNA or RNA, and specifically relates to a centrifuge tube structure for extracting DNA by a silica bead method. Background technique [0002] Isolation and purification is the extraction of DNA, whether in the fields of medical treatment, criminal case investigation, identification of accident victims, archaeology, etc., or in the field of animal and plant inspection and quarantine. DNA detection methods are mainly but not limited to the following : Phenol-chloroform extraction method, chelex-100 method; magnetic bead method; organic solvent method; salting out method and silica bead method, etc. However, no matter what method is used, the common features or common requirements can be roughly summarized into the following two aspects: one is to maximize the yield and purity of DNA or RNA as much as possible and to minimize the PCR reaction inhibitors; the sec...

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Problems solved by technology

For this reason, the applicant has searched the literature, and the Chinese patent authorization announcement number CN201744365U recommends a "filter membrane centrifuge tube". It can be seen from the description that there is no need to pipette during the operation, but due to the unreasonable structure, there are the following disadvantages: First, impurities are easy to enter the lower part of the centrifuge tube from the filter membrane (the patent is called a mixed fiber microporous filter membrane) with the body fluid, thus affecting the water-soluble The purity of the DNA template is affected; the second is that it is difficult to form an ideal seal between the rubber ring for the filter membrane and the inner wall of the centrifuge tube, so impurities will also enter from the micro-gap between the rubber ring and the inner wall of the centrifuge tube The lower part of the centrifuge tube, because the rubber ring taught by the patent must meet the requirements of being pluggable with the lumen wall of the centrifuge tube, so if in order to achieve the ultimate sealing effect between the rubber ring and the lumen wall of the centrifuge tube, Then it is difficult to remove the rubber ring together with the pressure ring and the filter membrane from the centrifuge tube through the hanging ring on the rubber ring in the subsequent operation steps. On the contrary, if the rubber ring is taken out in order to ensure convenience, it will inevitably affect the lumen of the rubber ring and the centrifuge tube. The sealing effect between the cavity walls; the third is that only one filter membrane cannot objectively reflect the good effect of filtering out impurities, thus affecting PCR amplification

[0006] Authorized announcement number CN202610231U provides a "multi-layer nucleic acid adsorption centrifuge sleeve". Because the principle of DNA extraction in this patent solution is opposite to that of the aforementioned patent, that is, the adsorption method is used. Although there is no need to pipette during the operation, the DNA template is lost. Difficult to control, please refer to column 0025 of the patent specification for details

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