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Pseudomonas pseudoalcaligenes and application thereof

A pseudomonas-like alkali-producing technology, which is applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems that the results cannot meet the actual needs of industrial production, and is simple to achieve nutritional needs. , Broad application prospects, the effect of reducing nitrate and nitrite

Active Publication Date: 2014-06-18
青岛玛斯特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, aerobic denitrification has attracted more and more attention, and there are many screening studies on aerobic denitrification bacteria at home and abroad, but the results are still far from meeting the actual needs of industrial production.

Method used

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  • Pseudomonas pseudoalcaligenes and application thereof
  • Pseudomonas pseudoalcaligenes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Isolation, screening and identification of Pseudomonas pseudoalcaligenes FX13

[0010] 1. Culture medium:

[0011] BTB medium ( / L): agar 20g, KNO 3 1g, KH 2 PO 4 1g, FeCl 2 ·6H 2 O0.5g, CaCl 2 ·7H 2 O0.2g, MgSO 4 ·7H 2 O1g, sodium succinate 8.5g, bromothymol blue (1% ethanol solution) 1mL, adjust the pH to 7.0-7.3 with 1mol / L NaOH, sterilize at 121°C for 20min, and set aside.

[0012] LB liquid medium ( / L): KNO 3 1g, KH 2 PO41g, FeCl 2 ·6H 2 O0.05g, CaCl 2 ·7H 2 O0.02g, MgSO 4 ·7H 2 O1g, sodium succinate 8.5g, sterilized at 121°C for 20min, set aside.

[0013] DM denitrification medium ( / L): KNO 3 0.72g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O1g, sodium succinate 2.8g, sterilized at 121°C for 20min, set aside.

[0014] 2. Screening method

[0015] Dilute the sludge sample to an appropriate concentration using the gradient dilution method, take 0.1mL and evenly spread it on the surface of the BTB medium, put it in a constant temperature incubator,...

Embodiment 2

[0023] Example 2: Degradation of aquaculture wastewater by Pseudomonas alcaligenes FX13

[0024] The Pseudomonas pseudoalcaligenes FX13 screened by the present invention is activated, expanded and cultivated, subjected to liquid fermentation, centrifuged to collect the bacteria sludge, added to a starch carrier, and dried to make 10 billion / g bacteria powder. The aquaculture wastewater was taken from Qingdao Tianhenghai Ginseng Pond. It was determined that the nitrate nitrogen content was 54.42±1.15mg / L and the nitrite nitrogen was 0.18±0.03mg / L. The experimental container was a plastic barrel and the experimental water body was 10L. According to the mass ratio of 10ppm and 50ppm, the Pseudomonas pseudoalcaligenes FX13 bacterial powder was added to the control group, and no bacterial powder was added to the control group. Three parallel sets were set up in the experimental group and the control group, and the aeration was uninterrupted during the experiment. To be consistent, ...

Embodiment 3

[0028] Example 3: Experiment on Degradation of Domestic Sewage Wastewater by Pseudomonas-like Alcaligenes FX13

[0029] Pseudomonas pseudoalcaligenes FX13 was activated, expanded and cultured. After liquid fermentation, the bacteria sludge was collected by centrifugation, added to starch carrier, and dried to make 10 billion / g bacteria powder. The domestic wastewater was taken from the drainage ditch of the vegetable market in Licun, Licang District, Qingdao City. After standing still for sedimentation, the supernatant was taken. The content of nitrate nitrogen was 168.34±3.67mg / L, and the content of nitrite nitrogen was 1.31±0.11mg / L. L, the experimental container is a plastic barrel, and the experimental water body is 10L. The experimental group is added with Pseudomonas pseudoalcaligenes FX13 bacterial powder according to the mass ratio of 10ppm and 50ppm, and the control group is not added with bacterial powder. , During the experiment period, the aeration was uninterrupte...

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Abstract

The invention discloses a pseudomonas pseudoalcaligenes and an application thereof. The pseudomonas pseudoalcaligenes FX 13 is screened from bottom mud of a penaeus vannmei culture pond, and the conservation number thereof is CCTCC NO: M2013069. The strain has aerobic and denitrification properties, and has an efficient degradation effect on nitrate and nitrite in water environment.

Description

technical field [0001] The invention belongs to the technical field of microbe application, and in particular relates to a strain of Pseudomonas pseudoalcaligenes and its application. Background technique [0002] In recent decades, my country's aquaculture industry has developed rapidly, and the scale of intensive and high-density farming has been expanding day by day. However, due to the accumulation of a large amount of residual bait, feces, and animal and plant corpses in the aquaculture water body, the nitrogen content in the water body seriously exceeds the standard, and the eutrophication intensifies, which destroys the aquaculture ecological environment, frequently causes fish and shrimp diseases, and eventually causes huge economic losses. . Studies have shown that the main cause of aquatic animal disease is the serious excess of nitrite nitrogen content in aquaculture water. Nitrite can oxidize ferrous hemoglobin in fish and shrimp, making it into methemoglobin, c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 武玉强王鹏
Owner 青岛玛斯特生物技术有限公司
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