Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of sphingomonas paucimobilis and its application

A technology of oligokinesis and monocysts, applied in the field of degrading bacteria, to achieve the effect of strong environmental adaptability and simple nutritional requirements

Active Publication Date: 2021-06-04
ZHEJIANG UNIV OF TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The work of screening microbial resources to degrade oil and restore crude oil-contaminated land has been reported at home and abroad, but the use of microorganisms in the treatment of food waste and its real application in daily life is still seldom

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of sphingomonas paucimobilis and its application
  • A kind of sphingomonas paucimobilis and its application
  • A kind of sphingomonas paucimobilis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Screening of bacterial strain YZ-4

[0027] 1. Culture medium:

[0028] Enrichment medium composition: peptone 10g / L, yeast powder 5g / L, glucose 20g / L, edible blend oil 5g / L, 6g / L Tween 80, solvent is deionized water, pH is natural. Sterilize at 115°C for 30 minutes.

[0029] The composition of acclimation medium: dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, sodium chloride 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, solvent is deionized Water, pH natural. Sterilize at 121°C for 20 minutes.

[0030] Oil screening medium composition: ammonium sulfate 2g / L, dipotassium hydrogen phosphate 1g / L, potassium chloride 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, ferrous sulfate 0.01g / L, agar 20g / L, Edible blended oil emulsion 12mL / L, solvent is deionized water, pH is natural. Sterilize at 121°C for 20 minutes. The edible blended oil emulsion is obtained by emulsifying the edible blended oil ...

Embodiment 2

[0040] Embodiment 2: Identification of bacterial strain YZ-4

[0041] 1. Colony morphology observation:

[0042] Use an inoculation loop to pick a small amount of preserved strains from the slant of Example 1, streak on the YPD plate, and culture at 37°C for 1-2 days. The colony morphology is shown in figure 1 . The colony is yellow, protruding, with a smooth and round surface.

[0043] 2. SEM scanning electron microscope observation:

[0044] Cells are rod-shaped, without capsule, flagella, spores ( figure 2 ).

[0045] 3. Molecular identification:

[0046] Template preparation: pick a single colony in a medium containing 20 μL sterile ddH 2 In the EP tube of O, cook in boiling water for 10 minutes, centrifuge at 12,000 rpm for 1 minute, and use the supernatant as a DNA template. 16S rDNA sequence was amplified by PCR with 27F (5'-AGAGTTTGATCCTG GCTCA-3') and 1492R (5'-AAGGAGGTGATCCAGCCGCA-3') as forward and reverse primers.

[0047] PCR amplification system (50 μL):...

Embodiment 3

[0062] Example 3: Application of Sphingomonas paucimobilis YZ-4 in degrading edible blended oil

[0063] 1. Culture medium:

[0064] With embodiment 1.

[0065] 2. Experimental method:

[0066] Pick a ring of the strain YZ-4 preserved on the slant and place it in a test tube containing 5mL LB liquid medium, culture at 37°C and 200rpm for 12-14h to obtain seed liquid; transfer 1mL of seed liquid to 100mL LB liquid culture medium In a 500mL Erlenmeyer shake flask based on the base, culture at 37°C and 150rpm for 12-14h to obtain a fermentation broth. The fermentation broth was centrifuged at 4°C and 8000 rpm for 10 min, and the wet cells were collected for later use.

[0067] pH optimization: adjust the pH (4, 5, 6, 7, 8, 9) of the inorganic salt medium with HCl and NaOH respectively, and inoculate the wet bacteria into the inorganic salt containing 40g / L edible blending oil according to the inoculation amount of 10g / L In the culture medium, react for 7 days at 37°C and 150r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
boiling pointaaaaaaaaaa
Login to View More

Abstract

The invention discloses a sphingomonas paucinobilis (Sphingomonas paucinobilis) YZ‑4 and its application in oil degradation. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M2018920. The sphingomonas paucimobilis isolated from the soil in the present invention has simple nutritional requirements and strong adaptability to the environment. It is a new type of microbial resource, which can effectively degrade the special component oil in the kitchen waste, and can be developed into a kitchen waste. The quick-degrading microbial agent for garbage has broad application prospects.

Description

[0001] (1) Technical field [0002] The invention relates to a degrading bacterium for grease in kitchen waste, in particular to Sphingomonas paucimobilis and its application in degrading grease. [0003] (2) Background technology [0004] With the rapid increase in the daily output of food waste in China, my country's traditional incineration and landfill technologies have been unable to solve the problems of low degradation efficiency of food waste, large floor area, and serious environmental pollution, which has caused my country to be in a "garbage besieged city" "The dilemma. Therefore, the application of composting technology in the treatment of food waste has become urgent. However, due to the refractory characteristics of oil, the main component of food waste, the degradation of oil has become the key rate-limiting step in the application of food waste composting. important. [0005] Tian Xingping (CN102586112A) used Bacillus and Pseudomonas as fat-degrading bacteria ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F9/04C12R1/01
CPCC05F9/04C12N1/205C12R2001/01Y02A40/20
Inventor 柳志强周海岩王艳梅薛亚平柯霞郑裕国
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com