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A way to overcome tumor drug resistance

A tumor and purpose technology, applied in the field of overcoming tumor drug resistance, can solve problems such as difficult to achieve effective reversal concentration, low drug utilization rate, toxic and side effects, etc., and achieve the effect of simple and easy material collection, enhanced killing effect, and simple nutritional requirements

Active Publication Date: 2021-08-17
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
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  • Claims
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Problems solved by technology

However, in further research, it is found that these reversal inhibitors are difficult to achieve the effective reversal concentration of in vitro experiments in vivo experiments, and the drug utilization rate is low; more importantly, due to poor targeting, reversal inhibitors will also interact with P-glycoprotein in healthy tissues. effect, causing the drug to produce toxic side effects on healthy tissues, so it cannot be put into clinical use

Method used

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  • A way to overcome tumor drug resistance
  • A way to overcome tumor drug resistance

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Experimental program
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Effect test

Embodiment

[0029] Prepare for the experiment: Prepare a 500ml Erlenmeyer flask, pour cyanobacteria and sterilized BG11 medium into the aseptic operating table, seal the bottle mouth with a breathable parafilm, and place it in the sun for cultivation and amplification.

[0030] Experiment 1: Increased oxygenation in vitro to increase the entry of doxorubicin into cells

[0031]Spread hypoxic breast cancer cells (MCF-7) and normal state breast cancer cells (MCF-7) into the confocal dish and put them into the incubator (the hypoxic cells are placed in the hypoxic incubator, the normal state The cells were cultured in a normal incubator) for 24 hours, and the cells in the hypoxic state were added with 2×10 7 cfu / mL cyanobacteria or no cyanobacteria, 660nm laser irradiation or no laser irradiation and culture for 24 hours, add DOX for co-incubation for 1 hour, stain the nucleus, and shoot confocal.

[0032] Confocal results such as figure 1 As shown, the cyanobacteria and laser irradiation ...

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Abstract

The invention discloses a new function of cyanobacteria, which can produce oxygen through photosynthesis to improve tumor hypoxia under the irradiation of laser with a specific wavelength, and can effectively overcome the resistance problems of doxorubicin and other chemical drugs. Because cyanobacteria perform photosynthesis under light to produce oxygen, under aerobic conditions, tumor cells can down-regulate hypoxic and hypoxic factors, which can inhibit multidrug resistance genes, inhibit the expression of P-glycoprotein, and inhibit the ability of cancer cells to doxorubicin. efflux, thereby overcoming the resistance of cancer cells to doxorubicin and improving the antitumor efficacy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for overcoming tumor drug resistance. Background technique [0002] Chemotherapy is one of the main methods for treating malignant tumors. The biggest advantage of chemotherapy compared with surgery and radiotherapy is that chemotherapy is a systemic treatment. Chemotherapy drugs can reach the whole body through the human circulatory system through oral administration, injection, etc., so they have a killing effect on primary cancer lesions, metastatic lesions, and even free circulating tumor cells. Through natural extraction, artificial synthesis and a large number of screening, many drugs have been approved by the US Food and Drug Administration for clinical application. At present, common chemotherapy drugs include adriamycin, 5-fluorouracil, camptothecin, paclitaxel, and etoposide. , Cismolybdenum, etc. However, while chemotherapy drugs kill tumor cells, they...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/748A61K45/06A61K31/704A61P35/00A61P35/04C12Q1/02
CPCA61K31/704A61K35/748A61K45/06A61P35/00A61P35/04G01N33/5011A61K2300/00
Inventor 蔡林涛何华美刘兰兰梁锐晶周海梅潘宏
Owner SHENZHEN INST OF ADVANCED TECH
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