A strain of Bacillus licheniformis and its application in aquaculture

A technology of bacillus licheniformis and aquatic feed, applied in the fields of application, bacteria, biological water/sewage treatment, etc., can solve problems such as excessive nitrogen content in water bodies, destruction of aquaculture ecological environment, frequent occurrence of fish and shrimp diseases, etc., and achieve reduction of ammonia state Nitrogen content, improved availability, simple effect on nutrient requirements

Active Publication Date: 2016-11-09
青岛玛斯特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, my country's aquaculture industry has developed rapidly, and the scale of intensive and high-density farming has been expanding day by day. Concentrated bait feeding has resulted in the accumulation of a large amount of residual bait, feces, and dead animals and plants in the aquaculture water, resulting in a serious excess of nitrogen content in the water body and eutrophication. The aggravation of globalization has caused the destruction of the aquaculture ecological environment, frequent occurrence of fish and shrimp diseases, and ultimately caused huge economic losses

Method used

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  • A strain of Bacillus licheniformis and its application in aquaculture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Isolation, screening and identification of strains

[0012] 1. Sample

[0013] Taken from the water sample in the treatment tower of salmon recirculating aquaculture wastewater of Yantai Oriental Ocean Group;

[0014] 2. Autotrophic nitrosation medium:

[0015] substance name

Dosage

NaCl

2.0g

MgSO 4 ·7H 2 O

0.5g

CaCO 3

5.0g

FeSO 4 ·7H 2 O

0.4g

K 2 HPO 4

1.0g

Agar

20.0g

H 2 O

Dilute to 1000.0mL

[0016] 3. Screening method

[0017] The water sample was diluted to an appropriate concentration by the gradient dilution method; 0.1 mL of the diluted water sample was evenly spread on the surface of the autotrophic nitrosation medium, placed in a constant temperature incubator, and incubated at 30°C for 2-3 days, then placed in an inoculating loop. Pick a single colony on the medium, and isolate and purify it, which is the primary screening strain.

[0018] A t...

Embodiment 2

[0032] Example 2: Bacillus licheniformis ZY-4 treatment of aquaculture water experiments

[0033] Bacillus licheniformis ZY-4 is activated, expanded and cultured, and is prepared into about 10 billion / g bacterial powder through liquid fermentation and spray drying.

[0034]The experiment was carried out in a small shed shrimp culture pond in Rudong, Jiangsu. Three culture ponds with similar water quality indicators were selected as three parallels, and the water bodies in the culture ponds were separated by plastic sheets. Half of the water bodies were used as the experimental group, and the other half as the experimental group. control group. Firstly, the NH in the experimental group and the control group were detected respectively. 4 + - the initial concentration of N, then add 10ppm of the above-mentioned Bacillus licheniformis ZY-4 bacteria powder to the water body of the experimental group, and the control group does not add it. During the experiment, the aeration was ...

Embodiment 3

[0039] The influence of embodiment 3 Bacillus licheniformis ZY-4 on feed-to-meat ratio of aquatic animals

[0040] The experiment was set up in a small shed shrimp culture pond in Rudong, Jiangsu Province. A pond with normal water quality and healthy prawns was selected. Six enclosures of 4m×4m×2m were built with plastic cloth in the cultivation pond, and the same amount of prawn was injected into the enclosure. The water in the pond has an equal amount of aeration stones in the enclosure to adjust the aeration evenly. Three enclosures were randomly selected as the experimental group, and the other three as the control group. Each group was fed with 1000 healthy Penaeus vannamei. Before feeding, 500 shrimps were randomly selected from each group for weighing, and the average value was taken for each of the three experimental groups and the control group.

[0041] The feed of Penaeus vannamei is commercially available prawn compound feed, wherein the feed of the experimental g...

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Abstract

The invention relates to the technical field of functional microorganism screening, and specifically provides a strain of bacillus licheniformis and its application in aquaculture. The strain has been preserved in the China Center for Type Culture Collection on June 24, 2014, and the number is CCTCC NO: M2014277. It has autotrophic and nitrosative properties, simple nutritional requirements, and can significantly reduce the content of ammoniacal nitrogen in water , the removal rate of ammoniacal nitrogen in the water after 48 hours of treatment was as high as 93.39%; in addition, the Bacillus licheniformis can be used as a feed additive in aquatic feed, which can effectively improve the utilization rate of the feed of Penaeus vannamei. The meat ratio was 14.3% lower than that of the control group. Therefore, the Bacillus licheniformis provided by the present invention can be developed into aquatic micro-ecological preparations and sewage treatment agents, which not only helps to improve the economic benefits of farmers, but also significantly reduces the pollution of water bodies caused by high-density farming, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Bacillus licheniformis and its application in aquaculture. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly, and the scale of intensive and high-density aquaculture has been expanding. Concentrated feeding has resulted in the accumulation of a large number of residual bait, feces and animal and plant carcasses in the aquaculture water, resulting in a serious excess of nitrogen in the water and eutrophication. The aggravation of the aquaculture has resulted in the destruction of the aquaculture ecological environment, the frequent occurrence of fish and shrimp diseases, and ultimately caused huge economic losses. Nitrosation is the conversion of ammonia nitrogen into nitrite nitrogen, which is an important part of the nitrogen cycle in nature. With the development of high-density aquacultu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34A23K10/18C12R1/10A23K50/80
Inventor 武玉强李书勋于虹张济福
Owner 青岛玛斯特生物技术有限公司
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