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Application of mesenchymal stem cells and its extraction method in the preparation of medicines for psoriasis

A technology of mesenchymal stem cells and psoriasis, applied in the application field of mesenchymal stem cells and its extraction in the preparation of psoriasis drugs, can solve the problem that the immune regulation function of skin mesenchymal stem cells has not been discovered

Active Publication Date: 2015-09-09
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
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Problems solved by technology

However, the immunoregulatory function of skin mesenchymal stem cells has not yet been discovered, and the present invention will conduct research on this issue

Method used

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  • Application of mesenchymal stem cells and its extraction method in the preparation of medicines for psoriasis
  • Application of mesenchymal stem cells and its extraction method in the preparation of medicines for psoriasis
  • Application of mesenchymal stem cells and its extraction method in the preparation of medicines for psoriasis

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Embodiment Construction

[0027] 1. Isolation of mouse dermal mesenchymal stem cells:

[0028] Take out the newborn mice about 3 days old, kill them by necking, disinfect with alcohol, cut the back skin and put it in 0.25% dispase enzyme, digest it overnight at 4°C, wash it with PBS solution for 3 times, peel off the epidermis, and place the dermis in 0.2% dispase After the digestion, add DNase I to digest for 10 to 15 minutes, wash it with PBS, blow it into a single cell suspension with a dropper, filter to remove large pieces of tissue and debris, and inoculate in a petri dish , the culture condition is DMEM (low glucose) + 10% fetal bovine serum, 37°C, 5% CO2. After culturing for 6 hours, discard the suspended cells gently, and continue culturing the adherent cells. After most of the bottom of the bottle is covered, trypsinize and routinely pass. Cells passed down to the third passage were taken for research.

[0029] 2. Identification of mouse dermal mesenchymal stem cells:

[0030] Dermal mesen...

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Abstract

The invention aims to provide an application of mesenchymal stem cells in the preparation of psoriasis treatment medicines. Skin-derived mesenchymal stem cells used in the invention have the advantages of easy obtaining, no immune repellency, high curative effect, difficult recurrence after disease heal, and small side effects.

Description

technical field [0001] The invention relates to the application of mesenchymal stem cells in the preparation of drugs with immune regulation function, especially the application of skin-derived mesenchymal stem cells (Skin-derived Mesenchymal Stem Cells) in the preparation of drugs for treating psoriasis. Background technique [0002] Psoriasis is a common immune-related, relapsing chronic skin inflammation characterized by dense inflammatory cell infiltration in the dermis, mainly T cells, DCs, natural killer T cells and macrophages . The epidermis of patients with psoriasis is hyperproliferative and cannot be differentiated normally, resulting in significantly thickened epidermis and increased scabs (Nestle et al., 2009). The exact cause of psoriasis is unknown, and a combination of environmental factors (such as infection, trauma, stress, etc.) and genetic factors determine an individual's susceptibility. There is currently no cure for psoriasis (Nestle et al., 2009). N...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/28A61P17/06A61P37/04C12N5/0775
Inventor 王宏林柯芳
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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