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Cyclizing metal iridium complex and application thereof

A complex, metal iridium technology, applied in the field of transition metal luminescent materials and bioanalysis, can solve the problem of rarely reported pH sensors of iridium complexes

Inactive Publication Date: 2015-05-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ligands such as 4,7-dihydroxy-1,10-phenanthroline and 4,4'-dihydroxy-2,2'-bipyridine have been widely used in the research of pH sensors of ruthenium complexes, but about iridium Complex pH sensors are rarely reported

Method used

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  • Cyclizing metal iridium complex and application thereof
  • Cyclizing metal iridium complex and application thereof
  • Cyclizing metal iridium complex and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Iridium complexes containing hydroxyl ligands are used as "OFF-ON" fluorescent sensors in solution: samples are accurately weighed on a balance to make approximately 1x10 -3 mol / L DMSO solution, respectively dilute the sample solution to 6x10 -6 mol / L DMSO / H 2 O (1 / 1, v / v) mixed solution was used to test its luminescence spectrum in pH 0-14 solution. When HCl is added to the DMSO / H of these complexes 2 O (v / v, 1 / 1) solution, the pH of the solution changed from 7-0, the fluorescence of complexes 1 and 3 decreased significantly, but complex 2 did not change significantly. When NaOH changed the pH of these solutions from 7 to above 14, the complexes showed the opposite phenomenon, the fluorescence of complexes 1 and 3 recovered 9-fold and 5-fold and the emission peaks were red-shifted by 9nm and 4nm, respectively. Under alkaline conditions, compound 2 showed a special phenomenon. When the concentration of NaOH was less than 30 times, the fluorescence gradually decreased w...

Embodiment 2

[0044] Iridium complexes containing amino ligands are used as "OFF-ON-OFF" fluorescent sensors in solution: samples are accurately weighed on a balance to make approximately 1x10 -3 mol / L DMSO solution, respectively dilute the sample solution to 6x10 -6 mol / L DMSO / H 2 O (1 / 1, v / v) mixed solution was used to test its luminescence spectrum in pH 0-14 solution. With HCl adjusting the pH from 7.5-0, the fluorescence intensity of compounds 4-6 decreased to almost 0 ( figure 2 ). The difference from the corresponding dihydroxy compounds is that when NaOH was added to the solutions of compounds 4, 5, and 6, the luminescence of these compounds decreased significantly, respectively. The reversibility study found that after six cycles, the degree of fluorescence weakening of the iridium complex containing amino ligands was reduced, and the stability was not as good as that of the dihydroxy complex. From pH-I / I 0 It can be seen from the figure that there is a platform in the reacti...

Embodiment 3

[0046] Cell imaging and toxicity experiment of some cyclized iridium complexes containing hydroxyl ligands and amino ligands: MTT method was used to test the activity of HepG2 cells treated with iridium complexes. HepG2 cells were grown in 96-well tissue culture plates at a density of 4×10 6 cells / well, cultivated for three days, treated with iridium complex for 24 hours, washed the culture plate twice with culture medium, then added MTT, cultured for another 4 hours, and used cells not treated with iridium complex as control. Relative cytotoxicity with the formula [OD sample -OD blank ] / [OD reference -OD blank ] × 100 (OD is the optical density) calculated as a percentage expressed. Each experiment was repeated three times. The complex used in the experiment is [(pq) 2 Ir(bpyOH 2 )] Cl, [(ppy) 2 Ir(bpy(NH 2 ) 2 )]Cl and [(bzq) 2 Ir(bpy(NH 2 ) 2 )] Cl. The experiment found that [(pq) 2 Ir(bpyOH 2 )] Cl, [(ppy) 2 Ir(bpy(NH 2 ) 2 )]Cl and [(bzq) 2 Ir(bpy(NH ...

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Abstract

The invention discloses a cyclizing metal iridium complex and an application thereof. The cyclizing metal iridium complex containing hydroxyl ligand or amino ligand has different luminescent properties in an entire pH range, the iridium complex containing the hydroxyl ligand is in off-on luminescent variation in a solution with the pH value of 0 to 14, the iridium complex containing the amino ligand is in off-on-off luminescent variation in the solution with the pH value of 0 to 14, and the luminescent property can be used for the pH sensing analysis test of the iridium complex. Partial low cell toxicity iridium complexes have obvious cell imaging and can be used for marking biomolecules.

Description

technical field [0001] The invention belongs to the field of transition metal luminescent materials and biological analysis, in particular to a cyclized metal iridium complex containing a hydroxyl ligand or an amino ligand and an application thereof. Background technique [0002] Intracellular pH plays a crucial role in regulating cellular functions, affecting cellular metabolism, growth, ion transfer, and homeostasis. Fluorescent signaling devices are increasingly used for pH measurement. In recent years, iridium complexes have attracted much attention due to their long excited state lifetime, high fluorescence efficiency and obvious color change. 4,4'-dihydroxy-2,2'-bipyridine (bpyOH 2 ) and 4,4'-diamino-2,2'-bipyridine (bpy(NH 2 ) 2 ) ligand deprotonation can greatly change the UV or fluorescence properties of the complex. In this study, the chlorine-bridged dimer [(C^N) 2 IrCl] 2 Reaction with excess N^N ligands to obtain a series of ionic metal iridium complexes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F15/00C09K11/06G01N21/64
Inventor 李梅金
Owner FUZHOU UNIV
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