Perfusion type bioreactor of double-layer circulating system and application method of perfusion type bioreactor

A technology of bioreactor and circulation system, which is applied in the direction of specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, biochemical equipment and methods, etc., and can solve problems such as the contradictory requirements for the circulation of culture fluid, Achieve rapid and uniform dissolution, promote the effect of release separation

Active Publication Date: 2013-08-14
SUN YAT SEN UNIV
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  • Claims
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Problems solved by technology

[0007] In order to overcome at least one defect described in the above-mentioned prior art, the present invention provides a perfusion bioreactor with a double-layer circulation system to solve the problem of contradictory requirements for the circulation of the culture fluid during the culture process of the cells, and can effectively ...

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  • Perfusion type bioreactor of double-layer circulating system and application method of perfusion type bioreactor
  • Perfusion type bioreactor of double-layer circulating system and application method of perfusion type bioreactor
  • Perfusion type bioreactor of double-layer circulating system and application method of perfusion type bioreactor

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Embodiment Construction

[0039] The accompanying drawings are for illustrative purposes only, and should not be construed as limitations on this patent; in order to better illustrate this embodiment, certain components in the accompanying drawings will be omitted, enlarged or reduced, and do not represent the size of the actual product; for those skilled in the art It is understandable that some well-known structures and descriptions thereof may be omitted in the drawings.

[0040] Such as figure 2 , 3 Shown, a kind of perfusion bioreactor of double-layer circulation system, wherein, comprise culture tank 5, be located at the outer layer circulation system outside culture tank 5, be located at the inner layer circulation system inside culture tank 5 and gas filling system;

[0041] The outer circulation system includes a waste liquid storage tank 1, a culture liquid storage tank 2, a three-way valve 3, a first peristaltic pump 4A, a second peristaltic pump 4B, a culture liquid introduction pipe 6, ...

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Abstract

The invention relates to the technical field of bioreactors in medical appliances, and more specifically relates to a perfusion type bioreactor of a double-layer circulating system and an application method of the perfusion type bioreactor. The perfusion type bioreactor of the double-layer circulating system comprises a culture tank, an outer layer circulating system arranged outside the culture tank, an inner layer circulating system arranged inside the culture tank, and a gas filling system. By adopting the perfusion type bioreactor, the problem of the contradictory requirement for circulation flow of a culture medium during the culture of cells is solved, the solubility and distribution uniformity of oxygen in the culture medium can be effectively improved, the discharge of the waste gas in the culture medium is facilitated, and air bubbles can be effectively prevented from entering a cell culture area.

Description

technical field [0001] The invention relates to the technical field of bioreactors in medical devices, and more specifically, to a perfusion bioreactor with a double-layer circulation system and an application method thereof. Background technique [0002] The structure of the existing bioreactor is as follows figure 1 As shown, its function is to create an environment similar to that in the body in vitro to accelerate the cultivation of cells and the formation of tissues and even organs. It can be widely used in patients with organ defects to replace, construct, maintain or enhance their tissue functions, as well as biopharmaceuticals, etc. field. The principle is to use engineering technology to construct a set of devices in vitro based on the physiological, physical, and chemical characteristics of the microenvironment in which cells grow in vivo, so that it can provide a microenvironment similar to that in vivo, so as to facilitate the growth of cells in this in vitro mi...

Claims

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Application Information

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IPC IPC(8): C12M1/02C12M3/00C12M1/04
Inventor 罗语溪高玉宝龚逸鸿蒋庆
Owner SUN YAT SEN UNIV
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