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Method for measuring methyl mercury and ethyl mercury in animal flesh

A technology of methylmercury and ethylmercury, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve problems such as fish pollution and methylmercury accumulation, and achieve the effects of good stability, good accuracy, and simple and fast measurement.

Inactive Publication Date: 2013-10-02
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU +1
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Problems solved by technology

However, mercury in aquatic systems is easily absorbed by aquatic organisms, causing methylmercury to accumulate in fish
Ultimately, even in water with low levels of methylmercury, high levels of mercury contamination in fish

Method used

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  • Method for measuring methyl mercury and ethyl mercury in animal flesh
  • Method for measuring methyl mercury and ethyl mercury in animal flesh
  • Method for measuring methyl mercury and ethyl mercury in animal flesh

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Embodiment Construction

[0040] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0041] In this embodiment, taking fish as an example, the method for measuring methylmercury and ethylmercury in fish comprises the following steps:

[0042] 1) Fish pretreatment: Mince the fish, pre-freeze at -75~-85℃ for 2.5~3.5 hours, put it in a vacuum freeze dryer, freeze it for 65~80h, crush it, and put it in the refrigerator at -18~-22℃ , to be analyzed and detected;

[0043] 2) Microwave-assisted extraction: Weigh 0.2 g of the above-mentioned fish meat into a microwave extraction tank, add 5.0 mL of hydrochloric acid with a concentration of 5 mol / L and 0.25 mol / L NaCl, and extract at 60°C for 10 min to obtain an extract;

[0044] 3) The extract is injected into a liquid chromatography-atomic fluorescence spectrometer for analysis:

[0045] Wherein liquid chromatographic separation is to select C18 reverse-phase column, mobile phase i...

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Abstract

The invention discloses a method for measuring methyl mercury and ethyl mercury in animal flesh. The method is characterized by comprising the following steps of: pretreating an animal flesh sample; performing microwave-assisted extraction; analyzing through a liquid chromatography-atomic fluorescence combined instrument; and finally, collecting and processing the data by using a data processor, and measuring the content of methyl mercury and ethyl mercury in fish flesh. The microwave-assisted extraction and high performance liquid chromatography-atomic fluorescence spectroscopy (HPLC-AFS) method are simultaneously applied to detecting the methyl mercury and ethyl mercury in animal flesh, and an extraction process is optimized; according to selection and concentration control of mobile phase in chromatographic analysis and reasonable control on concentration of hydrochloric acid in atomic fluorescence analysis, selection of reducing agents and lamp current, the process flow is simplified, the content of methyl mercury and ethyl mercury in the animal flesh can be rapidly and simply measured, and the detection method is simple and fast in measurement and high in stability and has the characteristics of high measurement sensitivity, high accuracy and high interference resistance.

Description

technical field [0001] The invention relates to a method for determining methylmercury and ethylmercury in animal flesh and fish. Background technique [0002] Seafood is the most basic source of methylmercury pollution for humans. 90% of methylmercury is absorbed by the gastrointestinal tract, enters the blood and is transported to the whole body by complexing with mercaptans. For oral exposure, the central nervous system is the primary target organ, especially during infant development. These toxic effects alter some sensory functions (vision, hearing), motor coordination, memory, concentration, and learning. Effects of low-dose mercury toxicity on different organ systems including: nervous system, motor system, renal system, cardiovascular system, immune system, and reproductive system. The most extreme examples are deaths in Minamata and Niigata, Japan, and Iraq. [0003] In riverbeds, the content of methylmercury in the total mercury will not exceed 25%, generally le...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 肖亚兵彭扬思高健会陈其勇
Owner ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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