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A kind of laser Raman detection method of malachite green

A malachite green and detection method technology, applied in the field of analysis and detection, can solve problems such as abnormal detection, complicated detection process, difficult and accurate judgment, etc., and achieve the effects of convenient product quality, food safety assurance, and practical detection technology

Inactive Publication Date: 2016-04-06
SHENZHEN YU CHI TESTING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this detection method are: 1. Malachite green becomes colorless after being metabolized by the fish body, and will not form a green ring; 2. This method is easily affected by other colored substances, and it is not easy to accurately judge whether the sample is really malachite green
The detection of this method needs to strictly ensure the activity of the enzyme. Once the enzyme activity is weakened or inactivated, the detection effect will not be obvious or even normal detection; in addition, the detection process is too complicated and not suitable for on-site detection

Method used

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  • A kind of laser Raman detection method of malachite green
  • A kind of laser Raman detection method of malachite green
  • A kind of laser Raman detection method of malachite green

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Prepare a 100μg / mL malachite green standard aqueous solution; prepare an enhanced chip: drop the nano-silver colloidal aqueous solution onto the aluminum foil, and after the addition is complete, the silver glue has a diameter of 3mm and dried to obtain an enhanced chip; drop 10μL of the malachite green standard aqueous solution Add it to the enhanced chip and dry to obtain the malachite green standard sample; use the American Ocean Optics RAM-PRO-785E portable laser Raman spectrometer to detect the malachite green standard sample to obtain the malachite green standard Raman spectrum. The main characteristic peak wave number is: 1176cm -1 ,1397.7cm -1 , And 1618.6cm -1 (See figure 2 ). Use the American Ocean Optics RAM-PINPNTR-785 handheld laser Raman spectrometer to scan the standard samples of malachite green (Raman spectrum scanning is performed to obtain the characteristic peak map of malachite green, the main characteristic peak wave number is: 1176cm -1 ,1397.7cm -...

Embodiment 2

[0045] Prepare a 0.2μg / mL malachite green aqueous solution; take 10μL dots on the silver gel enhanced chip, dry, and use the American Ocean Optics RAM-PRO-785E portable laser Raman spectrometer to detect the malachite green standard sample points to obtain malachite Green laser Raman spectrum (see Figure 4 ).

Embodiment 3

[0047] Detection of malachite green in fish.

[0048] Preparation of fish meat containing malachite green: add 5 g of crushed fish meat to a 50 mL centrifuge tube, and then add 0.5 mL of 2 ppm malachite green aqueous solution to the centrifuge tube, and stir with a glass rod for 15 minutes to fully absorb the mixed malachite green.

[0049] Extraction: Add 10 mL of acetonitrile to the above 50 mL centrifuge tube and homogenize for 30 seconds at 10000 r / min. Add 5 g of acidic alumina and shake for 2 minutes, centrifuge at 4000 r / min for 10 minutes, transfer the supernatant to a 125 mL separatory funnel, add 2 mL of diethylene glycol to the separatory funnel, and shake for 2 minutes.

[0050] Take a 50mL centrifuge tube and add 10mL acetonitrile, wash the homogenizer blade for 10 seconds, pour the washing solution into the previous centrifuge tube, use a glass rod to disperse the sediment in the centrifuge tube and stir evenly, vortex the mixer for 1 min, and let it stand for 20 min. ...

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Abstract

The invention provides a laser Raman detection method of malachite green, which comprises the following steps: preparing a reinforced chip: dropwisely adding a nano silver colloid water solution onto an aluminum foil, and after finishing the dropwise addition, drying to obtain the reinforced chip; dropwisely adding a sample extracting solution to be detected onto the reinforced chip, and airing to obtain a sampling point of the sample to be detected; and carrying out Raman spectrum scanning on the sampling point of the sample to be detected by using a laser Raman spectrograph, and carrying out qualitative detection on the sample to be detected according to the standard Raman spectrum of malachite green. The method is high in sensitivity, and is a convenient, quick and practical detection technique.

Description

Technical field [0001] The invention belongs to the technical field of analysis and detection, and specifically relates to a laser Raman detection method of malachite green. Background technique [0002] Raman spectra (Ramanspectra) is a kind of scattering spectrum. The Raman spectroscopy method is based on the Raman scattering effect discovered by the Indian scientist CV Raman in 1928. It analyzes the scattering spectrum with a frequency different from the incident light to obtain molecular vibration and rotation information, and applies it to molecules An analytical method for structural research. Raman inspection is more and more widely used in various inspection fields because of its fast, accurate and non-destructive advantages. [0003] Malachite green, also known as China green, is a toxic triphenylmethane chemical with metallic luster. It can be used as a dye for silk, leather and paper, as well as a biological dye. It is both a dye and a bactericidal agent. Agent, can c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 文秋红王莽毛燕
Owner SHENZHEN YU CHI TESTING TECH CO LTD
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