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Method for extracting, separating and preparing lignin monomers from schisandra chinensis

A technology of schisandra and lignans, applied in the field of natural medicinal chemistry, to achieve the effect of simple extraction steps, high product purity, and technological innovation and improvement

Active Publication Date: 2015-04-22
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no report on the separation and purification of lignan monomers in Schisandra chinensis by supercritical extraction and high-speed countercurrent chromatography

Method used

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  • Method for extracting, separating and preparing lignin monomers from schisandra chinensis
  • Method for extracting, separating and preparing lignin monomers from schisandra chinensis
  • Method for extracting, separating and preparing lignin monomers from schisandra chinensis

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1: supercritical fluid extraction

[0051] Take the fruit of Schisandra chinensis and crush it to get Schisandra medicinal material powder with a particle size of 50-65 mesh. Take 2 g of Schisandra medicinal material powder and put it into a supercritical fluid extraction tank. The extraction temperature is 40 °C, the extraction pressure is 25 MPa, CO 2 The flow rate is 2L / min, the extraction time is 1h, and the crude extract of Schisandra chinensis is obtained. Taking Schisandrin A as a representative, the calculated extraction rate is 0.5294%.

Embodiment 2

[0052] Embodiment 2: supercritical fluid extraction

[0053] Take the fruit of Schisandra chinensis and crush it to obtain the powder of Schisandra chinensis medicinal material with a particle size of 10-20 meshes. Take 3g of the medicinal material powder of Schisandra chinensis and put it into a supercritical fluid extraction tank. The extraction temperature is 35°C, the extraction pressure is 14MPa, CO 2 The flow rate is 2L / min, the extraction time is 2h, and the crude extract of Schisandra chinensis is obtained. Taking Schisandrin A as a representative, the calculated extraction rate is 0.3926%.

Embodiment 3

[0054] Embodiment 3: supercritical fluid extraction

[0055] Take the fruit of Schisandra chinensis and pulverize it to obtain the medicinal material powder of Schisandra chinensis. The particle size is 50-65 meshes. Take 3g of the medicinal material powder of Schisandra chinensis and put it into a supercritical fluid extraction tank. The extraction temperature is 35°C, the extraction pressure is 30MPa, CO 2 The flow rate is 2L / min, the extraction time is 2h, and the crude extract of Schisandra chinensis is obtained. Taking Schisandrin A as a representative, the calculated extraction rate is 0.6001%.

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Abstract

The invention discloses a method for extracting, separating and preparing lignin monomers from schisandra chinensis. The method comprises the following steps: performing supercritical extraction on schisandra chinensis medicinal powder by using CO2 to obtain a crude extract; performing primary high-speed counter-current chromatographic separation on the crude extract; forming a solvent system A by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (2-10):(0-10):(0-10):5, wherein the upper phase A is a stationary phase A and the lower phase A is a mobile phase A; preparing a first schizandrol, a second schizandrol and a third schizandrol; recovering the separated stationary A to serve as a sample to be tested; performing secondary high-speed counter-current chromatographic separation; forming a solvent system B by using hexane, ethyl acetate, methyl alcohol and water in the volume ratio of (1-10):(0-4):(0-9):1, wherein the upper phase B is a stationary phase B, and the lower phase B is a mobile phase B; preparing deoxyschizandrin and schisandrin b. By adopting the method which integrates supercritical extraction and high-speed counter-current chromatographic separation, the deoxyschizandrin and the schisandrin b can be directly used for separating and purifying subsequent monomers without complex sample treatment after the extraction; the steps of extracting and separating are simple and efficient.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry and relates to a fast and efficient combined preparation method for extracting, separating and purifying lignan monomer components from Schisandra chinensis. It specifically relates to the extraction and separation of monomer components in Schisandra chinensis by means of supercritical extraction combined with high-speed countercurrent chromatography. Background technique [0002] Schisandra chinensis is the mature fruit of Schisandra chinensis (Turcz.) Baill or Schisandra sphenanthera Rehd.et Wils. The former is commonly called "Northern Schisandra", and the latter is commonly called "Southern Schisandra". It was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Schisandra is warm in nature, sweet and sour in taste, and returns to the lung, heart and kidney channels. It has the effects of astringent and astringent, nourishing qi and promoting body fluid, invigor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D317/70C07C43/21C07C43/23C07C41/34C07C41/36C07B63/00
CPCY02P20/54
Inventor 楚楚颜继忠章诗迪童胜强李行诺
Owner ZHEJIANG UNIV OF TECH
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