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Application of galectin-4 protein in preparation of huge liver cancer postoperative prognostic evaluation kit

A prognostic evaluation and kit technology, which is applied in the field of Galectin-4 protein in the preparation of postoperative prognosis evaluation kits for giant liver cancer, can solve the problems of unclear molecular mechanism and lack of specific molecular targets for treatment

Active Publication Date: 2014-01-01
THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV +1
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  • Abstract
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  • Application Information

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Problems solved by technology

[0007] Liver cancer is one of the most threatening tumors to human health. So far, the molecular mechanism of its occurrence is still unclear, and its treatment lacks specific molecular targets. Galectin-4, as a very important tumor oncogene, is currently There is no literature report that Galectin-4 protein is related to judging the prognosis of huge liver cancer (ie giant hepatocellular carcinoma) or the metastasis of huge liver cancer

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  • Application of galectin-4 protein in preparation of huge liver cancer postoperative prognostic evaluation kit
  • Application of galectin-4 protein in preparation of huge liver cancer postoperative prognostic evaluation kit
  • Application of galectin-4 protein in preparation of huge liver cancer postoperative prognostic evaluation kit

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Effect test

Embodiment 1

[0045] (a) Pathological specimens come from biopsies or intraoperative and postoperative pathological materials from patients with giant liver cancer.

[0046] (b) Immunohistochemical method uses SP staining method, the specific steps are as follows:

[0047] (c) Paraffin sections of liver cancer tissues were prepared, and the oven was set at 60°C overnight.

[0048] (d) Deparaffinization of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[0049] (e) Slice hydration. Soak in sequence: absolute ethanol: 3min; 90% (v / v) ethanol: 3min; 80% ethanol: 3min; 75% ethanol: 3min.

[0050] (f) Wash with PBS 3 times, 5 min each time.

[0051] (h) EDTA antigen high-pressure repair: soak the slices in 0.01M EDTA repair solution, soak in boiling water for 5 minutes, and cool to room temperature. Wash 3 times with PBS, 5min each time.

[0052] (I) Add 300 μL of 3% (w / w) hydrogen peroxide aqueous solution, 37 ° C for 10 min. Wash 3 times with PBS, 5min e...

Embodiment 2

[0075] A postoperative hepatocellular carcinoma tumor sample from a giant liver cancer was taken for paraffin-embedded sectioning, and the above-mentioned immunohistochemical method was used for detection. After calculation, the Galectin4 immunohistochemical score of the cancer tissue was 0.085. After postoperative follow-up, it was found that the patient had recurrence and metastasis of liver cancer in the fifth month after the operation, and died seven months after the operation.

Embodiment 3

[0077] A postoperative hepatocellular carcinoma tumor sample of a huge liver cancer was taken for paraffin-embedded section, and detected by the above-mentioned immunohistochemical method. After calculation, the Galectin4 immunohistochemical score of the cancer tissue was 0.215. After postoperative follow-up, it was found that the patient was alive without metastasis and recurrence 1 year after the operation.

[0078] From the above test results, it can be known that detecting the relative expression of Galectin-4 protein molecules by immunohistochemical method can predict the risk of distant metastasis of giant hepatocellular carcinoma and the postoperative survival or death of patients. When the immunohistochemical score of Galectin-4 in cancer tissue is lower than 0.1545, giant hepatocellular carcinoma is prone to recurrence and metastasis, and patients with liver cancer are likely to die after surgery. Obviously, Galectin-4 protein is related to giant hepatocellular carcin...

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Abstract

The invention provides new application of galectin-4 protein in preparation of a huge liver cancer postoperative prognostic evaluation kit. Via wide and thorough researches, an inventor firstly discovers that detection of the relative expression level of the galectin-4 protein in a huge liver cancer tissue in an immunohistochemistry way can judge the risk of liver cancer recrudesce transfer of a huge liver cancer patient. The application has the main benefits as follows: the application of the galectin-4 protein in the preparation of the huge liver cancer postoperative prognostic evaluation kit, provided by the invention, points out that the galectin-4 protein can be used for preparing a protein molecular marker for prognosis of the huge liver cancer patient, and has important significance of guiding the huge liver cancer patient's postoperative monitoring and sequential treatment.

Description

(1) Technical field [0001] The invention relates to the application of Galectin-4 protein in the preparation of a postoperative prognosis evaluation kit for giant liver cancer. (2) Background technology [0002] Liver cancer is a serious liver disease, and its mortality rate ranks third among malignant tumors. According to research reports, in Asia and Africa alone, 500,000 people die from liver cancer every year. And my country is a country with a relatively high incidence of liver cancer. In recent years, the incidence and mortality of liver cancer in my country have been on the rise, and the population of patients is younger, which leads to a substantial increase in the country's annual expenditure on the treatment of liver cancer. The life, health and property safety of the masses constitute a great threat and affect the stability of the society. [0003] Huge HCC refers to a subtype of HCC with a diameter greater than 10 cm. Surgical resection is currently the first c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/57438G01N2800/7028
Inventor 刘景丰黄爱民刘小龙蔡志雄黄新辉
Owner THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
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