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Pathology application of schizocapsa total saponins

A technology of total saponins, split fruit potato, applied in the field of medical pathology

Inactive Publication Date: 2014-01-08
周莉 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the treatment of liver injury secondary to diabetes mellitus

Method used

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  • Pathology application of schizocapsa total saponins
  • Pathology application of schizocapsa total saponins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Determination of Inhibition Constant of CYP2E1 Enzyme Activity by Total Saponins of Dioscorea chinensis

[0025] CYP2E1 catalyzes the hydroxylation reaction of aniline, so aniline hydroxylase is the probe of CYP2E1, that is, measuring the activity of aniline hydroxylase can indicate the activity level of CYP2E1. The liver of normal animals was taken, and the activity of aniline hydroxylase was measured. The activity of aniline hydroxylase increased with the increase of the substrate concentration, showing the characteristic of saturation kinetics, which conformed to the Michaelis-Menten equation. Calculated K m The 95% confidence interval of the value is 22.18 to 43.24 μmol / L, V max The value was 0.48 μmol / min / mg.

[0026] According to the experimental results, the concentration of aniline was selected as 50 μmol / L, and the IC 50 Value (half inhibitory concentration) determination experiment. Dissolve total saponins of diosminum in water to form a concentration grad...

Embodiment 2

[0028] Experimental study on the protective effect of total saponins of diosminum on the liver injury secondary to diabetes mellitus

[0029] Compared with the control group, the activities of serum glutamate aminotransferase (sALT), aspartate aminotransferase (sAST) and CYP2E1 (aniline hydroxylase) in diabetic rats were significantly increased (all P<0.05), Indicates secondary damage to the liver.

[0030] Compared with the diabetic group, the activity of CYP2E1 in liver, serum glutamate aminotransferase (sALT) and aspartate aminotransferase (sAST) in the total saponins of split fruit potato group were significantly decreased (P < 0.05), indicating that the total saponins of split fruit potato It can protect the secondary liver damage caused by diabetes.

[0031] Table 2 The effect of total saponins of diosminum on diabetes secondary liver damage. n (number of cases) = 15, x ± s (mean ± standard deviation)

[0032]

[0033]

[0034] Note: *P<0.01; **P<0.05

Embodiment 3

[0036] In vitro study on the inhibition of high CYP2E1 activity secondary to diabetes mellitus by total saponins of diosminum

[0037] In diabetic rats, the CYP2E1 activity of liver microsomal, that is, the activity of aniline hydroxylase was 0.82±0.05 μmol / min / g, which was 2.3 times higher than that of the control group (0.35±0.12 μmol / min / g). In the in vitro incubation system, CYP2E1 activity in the diabetic group was significantly inhibited in a concentration-dependent manner by adding different concentrations of total diosgenins (the raw material drug of total diosmin was taken, diluted to different concentrations, and added to the in vitro incubation system).

[0038] The above experimental results show that the total saponins of diosminum can treat the liver function damage secondary to diabetes, and can be used for clinical treatment.

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Abstract

The invention relates to pathology application of schizocapsa total saponins in preparing medicine for treating liver damage secondary to diabetes. The schizocapsa total saponins is capable of inhibiting CYP2E1specifically so as to treat liver function damages secondary to the diabetes, and is a hepatoprotective medicine with high medical and economic value.

Description

technical field [0001] The invention relates to medical pathology, in particular to the use of total diosgenin in the preparation of medicines for treating impaired liver function secondary to diabetes. Background technique [0002] The liver is the main organ for the transformation and metabolism of various substances in the body. It expresses a variety of enzyme systems involved in the metabolism of substances, the most important of which is cytochrome P450 (CYPs for short). Among CYPs, subtype 2E1 (CYP2E1) mainly metabolizes small molecular compounds, including various drugs, halogenated hydrocarbons, alcohols, ketones, and endogenous substances such as arachidonic acid and fatty acids. CYP2E1 is considered to be an important determinant of the body's sensitivity to various toxic damage and carcinogenicity, especially in mediating liver damage. It is known that in the liver lobule, the expression level of CYP2E1 in hepatocytes located around the central vein is 30 times ...

Claims

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Application Information

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IPC IPC(8): A61K36/88A61P3/10A61P1/16
Inventor 周莉王敏
Owner 周莉
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