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Method for synchronously determining contents of tetravalent vanadium and pentavalent vanadium in culture medium in situ

A medium formula and medium technology, which is applied in the direction of color/spectral property measurement, etc., to achieve the effect of easy access to conditions and simple operation.

Inactive Publication Date: 2014-01-15
SICHUAN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to timely and accurately reflect the redox ability of microorganisms to vanadium in different valence states in the medium, an in situ method for simultaneous determination of tetravalent and pentavalent vanadium in the medium is particularly meaningful, but there is no such method yet

Method used

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  • Method for synchronously determining contents of tetravalent vanadium and pentavalent vanadium in culture medium in situ
  • Method for synchronously determining contents of tetravalent vanadium and pentavalent vanadium in culture medium in situ
  • Method for synchronously determining contents of tetravalent vanadium and pentavalent vanadium in culture medium in situ

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Embodiment Construction

[0017] Main reagent configuration

[0018] 1. Tetravalent vanadium standard solution: VOSO with 20% vanadium content 4 (Molecular weight 163) 0.25g, concentrated phosphoric acid 1ml to 500 ml (0.1 mg·L -1 );

[0019] 2. Pentavalent vanadium standard solution: Na 3 VO 4 12H 2 O (molecular weight 400) 0.4g, dilute to 500 ml (0.1 mg·L -1 );

[0020] 3. Phosphotungstic acid application solution (color developer) ： Na 2 WO 4 2H 2 O (molecular weight 330) 132g, dissolved in 300 ml of water, adjusted to pH = 3 with phosphoric acid, set to 500ml (0.8M);

[0021] 4. Adjusted beef extract peptone medium: 4 g peptone, 2 g beef extract, 5 g sodium chloride, 500 ml distilled water pH=7.4.

[0022] Operating procedures

[0023] Preparation of standard reference absorbance curve in medium: (8.5-a) ml deionized water, 0.1 ml vanadium-free medium, a ml (0.4figure 1 ). Pentavalent vanadium records the absorbance value at 420 nm and draws the standard curve , to get the absorbanc...

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Abstract

The invention discloses a method for synchronously determining contents of tetravalent vanadium and pentavalent vanadium in a culture medium in situ; and the method is used for researching the strength of the oxidization effect of microorganisms to the tetravalent vanadium and the reduction effect to the pentavalent vanadium in the beef extract peptone fluid culture medium. The method builds a chromogenic reaction system (comprising reaction reagent amount, adding sequence, chromogenic time and other conditions) through regulating the formula of the culture medium, and compares the changes of absorbance curves of the culture medium before and after the microorganism effects by using the spectrophotometry to achieve the synchronous in-situ determination of the tetravalent vanadium and the pentavalent vanadium in the culture medium. The method has the characteristics of low equipment requirements, simple operation, accurate determination, good reproducibility and the like, and is specifically suitable for qualitatively and quantitatively researching the redox capcity of the microorganisms to the vanadium.

Description

technical field [0001] The invention relates to a method for measuring tetravalent and pentavalent vanadium in a culture medium. The method realizes the in-situ simultaneous determination of the tetravalent and pentavalent vanadium in the culture medium, and is used for qualitative and quantitative determination of the oxidation-reduction effect of microorganisms on vanadium. Background technique [0002] As an environmental heavy metal pollutant, vanadium is attracting more and more people's attention, and reports related to vanadium continue to appear. In addition to hoping to understand the situation of vanadium content in the environment, people begin to study the different valence states of vanadium. Vanadium has a wide range of oxidation-reduction reactions in nature, and a very important part of it is completed by microorganisms. Therefore, it is of great significance to identify which microorganisms can carry out the oxidation-reduction effect of vanadium and the int...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 杨杰杨金燕李廷强王斌
Owner SICHUAN UNIV
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