Fully automatic immunochemical instrument and its application method

Abstract

The invention relates to the fields of life science and medical research, and discloses a full-automatic immunochemical instrument and a use method thereof. According to the invention, the full-automatic immunochemical instrument can realize automatic liquid charging and waste liquid suction in the whole process. By means of changing the liquid without changing a vessel hole, damage or position shift to a tissue slice sample due to frequent movement of the sample can be avoided in an operation process. Also, experimenters can avoid suffer body injury caused by direct contact with toxic substances. By automatic instrument operation, the labor intensity is alleviated, the efficiency is improved, artificial error can be avoided, and automation and standardization can be realized. A liquid distribution and liquid discharge structure and a liquid pipeline can move up and down, can drop during liquid charging and liquid suction, and rise during reaction vessel change, so that the operation can be more convenient. The liquid distribution and liquid discharge structure and the liquid pipeline can move around, batch treatment can be carried out on a reaction vessel hole matrix, and the efficiency can be higher.

Description

technical field [0001] The invention relates to the fields of life science and medical research, in particular to the fully automatic immunohistochemical technique for pathological tissue slices and the fully automatic immunocytochemical technique for culturing cells. Background technique [0002] Immunochemical staining, including immunohistochemistry and immunocytochemistry, is a classic, routine and rigorous laboratory work. Immunochemistry is very sensitive to protein changes in tissues and cells, and can be localized and semi-quantitative. Very valuable detection technology means. Immunohistochemical staining technology has two main methods for the existence of tissue sections: patch method and floating (floating) method. The floating film method refers to the tissue slices floating and free in the liquid for incubation and reaction, and after the incubation process is completed and the color is reacted, the tissue slices are then mounted on a glass slide. The advanta...

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Problems solved by technology

[0005] 1. Time-consuming and labor-intensive: each immunochemical operation takes at least 7 to 8 hours, or even more than 1 to 2 days, with many steps and boring; each experiment can only handle limited samples, such as 5 to 7 cases;

[0006] 2. Error-prone: There are more than a dozen types of antibodies and reagents used in each immunochemical operation, and it is easy to add by mistake or forget to add in manual operation, and the reaction time cannot be accurately controlled and standardized;

[0007] 3. Between different experimental batches, due to the influence of multiple factors, it is difficult to achieve complete reproduction and fitting, and it is difficult to eliminate the differences between batches

[0008] 4. Tissue slice samples are easy to damage: During manual operation, tissue slice samples need to be moved frequently, which is easy to cause damage to them, and it is easy to cause sample strings with the action of pulling in and out;

[0009] 5. Damage to health: Some of the antibodies and reagents used in immunochemical operations are highly toxic, such as diaminobenzidine (DAB) is a clear and strong carcinogen. During the manual operation, the experimenters are directly exposed to harmful liquids, which will damage the body. healthy

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