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A kind of natural immune adjuvant and vaccine composition and preparation method thereof

A vaccine composition and natural immunity technology, applied in the field of animal vaccine adjuvant, can solve the problems of poor immune protection effect and low level of immune response generation, and achieve the effects of environmental protection in the preparation process, improvement of immune response level and cost saving.

Active Publication Date: 2019-05-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, these adjuvant components have a series of problems such as low level of induced immune response and poor immune protection effect when used alone as anti-inflammatory drugs under the existing preparation technology.

Method used

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  • A kind of natural immune adjuvant and vaccine composition and preparation method thereof
  • A kind of natural immune adjuvant and vaccine composition and preparation method thereof
  • A kind of natural immune adjuvant and vaccine composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0029] Grind 20g of purified propolis (with flavonoid content > 98%) into fine powder, then add 95% ethanol to 60ml (this example is only the proportion ratio) to prepare a propolis ethanol solution with a concentration of 280mg / ml, so that the propolis particle size Uniform and stable, take 10ml of the above solution and slowly add it to low-temperature sterilized water (temperature lower than 8°C), keep stirring, and set the volume to 200ml, with a final concentration of 14mg / ml as the propolis component of the compound adjuvant for later use. In addition, 280 mg of saponin was weighed, and the volume was adjusted to 20 ml with sterilized water, and its final concentration was 14 mg / ml. After sterilizing and filtering, it was used as the saponin component of the adjuvant for future use.

[0030] In order to prove that the immune effect of the new compound natural adjuvant of the present invention is better than that of the previous natural adjuvant, we take the Newcastle dise...

Embodiment 2

[0033] The compound adjuvant of the present invention is prepared from two natural substances at a specific concentration, and the two components can be formulated into a concentrated solution with a high concentration. The proportion of the vaccine has the effect of concentrating the vaccine and improving the immune response level of the vaccine. In order to verify this advantage of the adjuvant, the following experiments were carried out:

[0034] Grind 20g of purified propolis (with flavonoid content > 98%) into fine powder, then add 95% ethanol to 60ml (this example is only the proportion ratio) to prepare a propolis ethanol solution with a concentration of 280mg / ml, so that the propolis particle size Uniform and stable, the propolis component used as a compound adjuvant is ready for use. In addition, 2800 mg of saponin was weighed, and the volume was adjusted to 10 ml with sterilized water, and its final concentration was 280 mg / ml. After sterilizing and filtering, it wa...

Embodiment 3

[0037] The compound adjuvant of the present invention is formulated by two kinds of natural substances by specific concentration, in order to illustrate the relationship between the immunoenhancing effect of the adjuvant and the concentration of propolis and saponin in the adjuvant, the following experiment is carried out:

[0038]Grind 20g of purified propolis (with flavonoid content > 98%) into fine powder, then add 95% ethanol to 60ml (this example is only the proportion ratio) to prepare a propolis ethanol solution with a concentration of 280mg / ml, so that the propolis particle size Uniform and stable, take 10ml of the above solution and slowly add it to low-temperature sterilized water (temperature lower than 8°C), keep stirring, and set the volume to 100ml, with a final concentration of 28mg / ml as the propolis component of the compound adjuvant for later use. In addition, 280 mg of saponin was weighed, and the volume was adjusted to 10 ml with sterilized water, and its fi...

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Abstract

The invention discloses a natural immunologic adjuvant and a vaccine composition thereof, as well as a preparation method. The natural immunoligc adjuvant is a compound adjuvant at least containing saponin and propolis, and the vaccine composition is prepared by the following steps: dissolving the saponin in water to prepare a saponin solution; dissolving propolis in 95 percent ethanol to prepare a propolis ethanol solution; uniformly mixing the saponin solution and antigen according to a proportion; adding the propolis ethanol solution or water diluted propolis solution. According to the natural immunologic adjuvant, the vaccine composition thereof and the preparation method, the saponin and the propolis are combined according to a certain proportion for the first time, and a unique process is adopted to prepare the natural compound adjuvant, the immune response level of the natural immunologic adjuvant is greatly increased compared with that of a single-component natural adjuvant, and the immune effect is reinforced.

Description

technical field [0001] The invention relates to the field of animal vaccines, in particular to animal vaccine adjuvants. Background technique [0002] "Adjuvant", that is, adjuvant, originated from the Greek word "adjuvare", which means to help. Immunological adjuvants refer to substances that can be applied simultaneously or in advance with antigens to enhance the body's immune response to antigens or change the type of immune response. There are many immune adjuvants commonly used, including oil emulsifiers, metal compounds, natural or synthetic bacterial products, nucleic acid sequences, liposomes, and immune stimulating complexes, etc., but all have different defects (such as unsatisfactory immune effects or toxic side effects). Large, etc.), which limits its application and development. [0003] At present, domestically used livestock and poultry vaccines are still dominated by traditional oil adjuvant vaccines, which have a series of serious problems such as poor imm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/00A61K39/39A61P37/04A61K35/64A61K35/644
Inventor 胡艳欣
Owner CHINA AGRI UNIV
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