A kind of non-carrier adjuvant for vaccine, its preparation method and application

A carrier and adjuvant technology, applied in the field of biomedicine, can solve the problems of strong toxicity, poor ability to stimulate immune response, affecting normal cell metabolism, etc., and achieve the effect of low cytotoxicity and good biocompatibility

Active Publication Date: 2019-03-12
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned nano-vaccine and adjuvant can be taken up by cells, affecting the normal metabolism of cells
[0005] Xu Ligeng et al. studied the role of gold nanorods modified by CTAB, PDDAC and PEI as DNA vaccine adjuvants in the treatment of HIV (see Ligeng Xu, et al., Surface-Engineered Gold Nanorods: Promising DNA Vaccine Adjuvant for HIV-1 Treatment .Nano Letters, 2012,12 (4): 2003-2012), although it disclosed for the first time that gold nanorods are used as a carrier adjuvant to enhance HIV immune response, yet, it has the following defects: gold nanorods adjuvant, to Cells are more toxic and less able to stimulate an immune response

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of non-carrier adjuvant for vaccine, its preparation method and application
  • A kind of non-carrier adjuvant for vaccine, its preparation method and application
  • A kind of non-carrier adjuvant for vaccine, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Synthesis and characterization of silver nanorods

[0049] Preparation of silver nanorods:

[0050] At room temperature, 0.5ml 3M AgNO 3 Add 2.5ml 2M PVP to 25mL polyethylene glycol with continuous stirring, and continue stirring for 5 minutes before heating. The mixture was heated to 80°C for 1 h, and continued to be heated to 90°C for 20 h. After cooling, use a large amount of ethanol or water to precipitate, slowly pour the upper liquid, add water to dissolve the precipitate, and ultrasonically in a water bath at 20°C to obtain silver nanorods.

[0051] The prepared silver nanorods were characterized by FIRT, TEM and DLS, and the results are shown in Figure 2 (A), (B), (C), (D) and (E).

[0052] It can be seen from the FIRT image of Figure 2(A) that the silver nanorods have two different peaks at 400nm and 900nm, indicating that they belong to a rod-like structure; from the TEM image of Figure 2(B), it can be seen that the diameter of the silver nanorods is about...

Embodiment 2

[0058] Preparation of silver nanorods:

[0059] At room temperature, 0.5ml 1M AgNO 3 Add 2.5ml 0.5M PVP to 25ml polyethylene glycol with continuous stirring, and continue stirring for 5 minutes before heating. The mixture was heated to 80°C for 1 h, and continued to be heated to 90°C for 20 h. After cooling, use a large amount of ethanol or water to precipitate, slowly pour the upper liquid, add water to dissolve the precipitate, and ultrasonically in a water bath at 20°C to obtain silver nanorods.

[0060] The prepared silver nanorods have a diameter of about 10 nm and a length of about 150 nm.

Embodiment 3

[0062] Preparation of silver nanorods:

[0063] At room temperature, 0.5ml 5M AgNO 3 Add 2.5ml 6M PVP to 25ml polyethylene glycol with continuous stirring, and continue stirring for 5 minutes before heating. The mixture was heated to 80°C for 1 h, and continued to be heated to 90°C for 20 h. After cooling, use a large amount of ethanol or water to precipitate, slowly pour the upper liquid, add water to dissolve the precipitate, and ultrasonically in a water bath at 20°C to obtain silver nanorods.

[0064] The prepared silver nanorods have a diameter of about 120nm and a length of about 300nm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
diameteraaaaaaaaaa
lengthaaaaaaaaaa
Login to view more

Abstract

The present invention relates to a non-carrier adjuvant for vaccines, a preparation method and applications thereof, wherein the non-carrier adjuvant is silver nano-rods with the surface modified by immunomodulatory molecules. According to the present invention, with the non-carrier adjuvant, the immune reaction level between the antibody IgG and the antibody IgG3 of the HIV protein vaccine, and the cell CD107a, the IL-2, the IL-4 and the TNF-alpha can be effectively enhanced; the cytotoxicity of the non-carrier adjuvant is extremely low, and the non-carrier adjuvant is almost not be uptaken by cells so as not to affect the normal cell growth; and the non-carrier adjuvant has good biocompatibility and important application values.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a non-carrier adjuvant for vaccines, its preparation method and application. Background technique [0002] Vaccine is an important means to prevent and control the occurrence and development of infectious diseases. Immune adjuvants (also known as non-specific immune proliferative agents) are an indispensable component of vaccines, which can effectively promote the specific immune response induced by the vaccine. With the rapid development of vaccine research, new genetic engineering vaccines are being widely used in vaccine research and development. This vaccine has the advantages of high purity and strong specificity, but due to its small molecule and relatively poor immunogenicity, there is an urgent need to combine an effective immune adjuvant to improve the immune efficacy of the vaccine. In the past decades, people have discovered and developed various new types of immune adjuvants i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61P37/04
Inventor 蒋兴宇刘野耶库尼·巴拉钱德兰
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap