Gene expression profile based drug toxicity individual-susceptible gene marker screening method

Abstract

The invention discloses a gene expression profile based drug toxicity individual-susceptible gene marker screening method. The method comprises the following steps: choosing individuals susceptible and tolerable to a drug toxicity according to the drug toxicity susceptibility difference of individuals to respectively compose a susceptible subset and a tolerance subset; choosing differentially expressed genes before administration according to the gene expression profiles of the above two subsets before the administration; further screening the differentially expressed genes before the administration according to the gene expression profiles of the two subsets after the administration to obtain an individual-susceptible latent gene marker capable of indicating the drug toxicity; and verifying the latent gene marker, and screening to obtain individual-susceptible gene marker capable of indicating the drug toxicity. The individual susceptibility of drug toxicities is researched on the basis of the gene expression profile, so the method is reliable and has a strong maneuverability.

Description

technical field [0001] The invention relates to the research on individual susceptibility to drug toxicity in pharmacogenomics, in particular to a screening method for gene markers of individual susceptibility to drug toxicity based on gene expression profile. Background technique [0002] Pharmacogenomics is a new discipline to determine the effect of individual genetic differences on drug effects. It has been widely used in the research of rational drug use and individualized drug administration. The individual diversity of drug response from phenotype to genotype, using known genomics theory to guide clinical rational drug use and innovative drugs. [0003] Due to the popularization of high-throughput DNA / RNA technology, exploring the role of various biomolecules such as DNA, mRNA, siRNA, and miRNA in the individual diversity of drug responses has gradually become a research hotspot. [0004] Existing studies have shown that differences in gene expression before administ...

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Problems solved by technology

However, looking at the existing studies, none of the differentially expressed genes before drug administration may have nothing to do with drug effects, and because of the large number of differentially expressed genes before drug administration, individual susceptibility gene markers for potential drug toxicity are screened. When using drugs, it is time-consuming, laborious and expensive to verify the differential genes one by one based on the difference in gene expression before administration. Therefore, it is necessary to further screen differentially expressed genes related to drug effects and explore them as markers of individual susceptibility to drug toxicity. possibility of things

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