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Method for carrying out ecological remediation in cadmium-contaminated soil by using cadmium resistant fungi

A cadmium-contaminated soil and ecological restoration technology, applied in the field of microbial ecological restoration, can solve the problems of low compatibility between microorganisms and plants, poor adaptability of microorganisms to soil, and low survival rate of microorganisms, so as to overcome adaptability problems, rapid microbial growth, The effect of easy operation

Active Publication Date: 2014-02-05
HUBEI CHU BANG SOURCE ECOLOGICAL AGRI CO LTD
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AI Technical Summary

Problems solved by technology

[0012] ① Microorganisms have poor adaptability to soil;
[0013] ② The compatibility between microorganisms and plants is low;
[0014] ③ Low microbial survival rate

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0033] Below in conjunction with embodiment describe in detail:

[0034] 1. Method

[0035] ①Isolation and purification of cadmium-resistant fungi

[0036] Select 10g of Bermudagrass rhizosphere soil from the heavy metal pollution area in Zhuzhou, China, and place it in a triangular flask. Under sterile conditions, add an appropriate amount of glass beads and 90ml of sterile water, and shake in a rotary shaker at 150rpm and 30°C for 30min, so that microorganisms can be removed from the soil. fully eluted in the medium, and then let it stand for 20 minutes, draw the supernatant and dilute 100 times with sterile water, which is the soil bacterial suspension; take 1ml of the supernatant and evenly spread it on a solid plate with a cadmium concentration of 50mg / L (beef Paste peptone medium, Martin's agar medium, Gao's No. 1 medium), after the colony grows, it is inoculated into solid medium with cadmium concentration of 100, 150, and 200 mg / L by plate streak separation method, 30...

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Abstract

The invention discloses a method for carrying out ecological remediation in cadmium-contaminated soil by using cadmium resistant fungi, and relates to a microorganism ecological remediation technology. The method comprises the following steps: (1) separating and purifying cadmium resistant fungi; (2) identifying the cadmium resistant fungi; (3) determining the most suitable growth condition of the cadmium resistant fungi; (4) preparing cadmium resistant fungus remediation matrixes; and (5) remedying the cadmium-contaminated soil. The method has the advantages that rhizosphere soil of dominant plants in a cadmium-contaminated area is used as a cadmium resistance microorganism screening source, and the defect of the adaptation of cadmium resistance microorganisms screened out to the soil environments in the remedied area is overcome; bermuda grasses which have a certain capacity of resisting cadmium and are compatible with the cadmium resistant fungi are used for overcoming the repulsive interaction between plants and microorganisms; the cadmium resistant fungi are inoculated in saw dust for application, so that the nutrient supply of microorganism growth is ensured, and the waste is effectively utilized; the method is low in cost, simple and convenient to operate, and the microorganisms rapidly grow and can breed on a large scale.

Description

technical field [0001] The invention relates to the technical field of microbial ecological restoration, in particular to a method for ecological restoration in cadmium-contaminated soil by using cadmium-resistant fungi. Background technique [0002] Cadmium is one of the most toxic heavy metal elements, which is extremely harmful. Excessive cadmium in the soil will inhibit the normal growth of plants, and the residue in the edible part will also affect human health through the food chain. In recent years, cadmium pollution incidents have occurred frequently, such as Beijiang in Guangdong, Longjiang in Guangxi, Zhuzhou in Hunan, Liuyang in Hunan, and cadmium-contaminated rice in Japan are shocking. The treatment of cadmium-contaminated soil has also aroused widespread concern at home and abroad. [0003] The use of engineering, physical and chemical methods to remediate cadmium-contaminated soil has certain limitations, is difficult to deal with on a large scale, and will ...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 傅金民罗宏基娄燕宏
Owner HUBEI CHU BANG SOURCE ECOLOGICAL AGRI CO LTD
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