A fully automatic western blot analyzer and its use and application

Abstract

The application of the invention provides a full-automatic immuoblotting analysis meter. The full-automatic immuoblotting analysis meter comprises an enclosure, wherein a control device, a support device, a peristaltic pump device, a shaking table device, a drying and absorption liquid adding device and a reading device are arranged in the enclosure, the control device comprises a power supply and a control circuit board, the support device is arranged in the enclosure and comprises a shell, a stepping motor and a synchronous belt driven by the stepping motor, the stepping motor and the synchronous belt are arranged in the shell, the synchronous belt drives a first fixed block and a second fixed block, the first fixed block drives the drying and absorption liquid adding device, the second fixed block drives the reading device, the peristaltic pump device is arranged on the support device, the shaking table device is arranged below the front of the support device, the drying and absorption liquid adding device is arranged above a tray rack and is connected with a synchronous belt of the support device through the first fixed block, and the reading device is arranged above the tray rack, is connected with the synchronous belt of the support device through the second fixed block, and comprises a camera, a light source and an input module for inputting acquired data into a computer for processing.

Description

technical field [0001] The application of the present invention relates to a fully automatic immunoblotting analyzer and its use method and application, belonging to the technical field of medical detection equipment. Background technique [0002] Immunoblotting, also known as Western blotting, is a method for detecting a certain protein in a complex sample based on the specific binding of antigens and antibodies. This method is a new immunobiochemical technique developed on the basis of gel electrophoresis and solid-phase immunoassay techniques. Due to the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassay, Western blot has become a routine technique for protein analysis. Western blotting is often used to identify a certain protein, and can perform qualitative and semi-quantitative analysis of the protein. Combined with chemiluminescent detection, it can simultaneously compare the expression differences of the same protein in mu...

AI Technical Summary

Problems solved by technology

At present, there are two types of Western blot detection: manual detection and instrumental detection. The manual detection method needs to replace different reagents, frequent operations increase the work intensity of the experimenter and waste time, and the manual operation is cumbersome and complicated. There is a lot of contact with potentially infectious items, and the detection safety is poor; although the western blot detection instrument currently used in clinical practice is called "automatic", it cannot actually complete the drying function of the test sample, and after the reaction is over, it needs to be It is time-consuming, laborious and inconvenient to remove the test sample from the instrument for additional testing and analysis

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