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Grid positioning sheet capable of detecting sperms quickly

A positioning sheet and grid technology, applied in the field of cell biology research, can solve the problems of sperm smear preservation without research value, unfavorable sperm morphological contrast observation and repeated measurement, unable to fix sperm positioning, etc.

Inactive Publication Date: 2014-03-12
徐元诚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current general-purpose sperm morphology smear cannot locate the attached fixed sperm, which is not conducive to the comparative observation and repeatable measurement of sperm morphology, making the preservation of sperm smears worthless for research

Method used

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  • Grid positioning sheet capable of detecting sperms quickly
  • Grid positioning sheet capable of detecting sperms quickly

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Embodiment Construction

[0013] In order to make the technical solution and characteristics of the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments and accompanying drawings. Here, the following examples are used to illustrate the present invention, but not to limit the scope of the present invention.

[0014] like figure 1 , figure 2 As shown, the grid positioning sheet of the present invention includes a glass substrate 1 and a polished glass slide 2, wherein the glass substrate 1 and the polished glass slide 2 are bonded together, and a square grid area 3 is drawn on the polished glass 2 .

[0015] The square grid area 3 has 25 large grids of 1 mm × 1 mm at intervals of 0.1 mm, and each large grid is marked with English capital letters A-Y on the upper left, and the line width of the large grid is 4-5 μm.

[0016] Each large grid has 100 small square grids of 0.1mm×0.1mm, which are marked with Arabic numerals 0-9 from...

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Abstract

The invention belongs to the field of cytobiology research, and specifically belongs to the field of sperm morphology positioning research. A grid positioning sheet disclosed in the invention comprises a glass substrate, and a polished glass sheet; the glass substrate and the polished glass sheet are glued together; the polished glass sheet is provided with a square grid zone via drawing; the square grid zone comprises 25 1mmX1mm big grids arranged with intervals of 0.1mm; the top left of each big grid is marked with a capital letter selected from A to Y; each big grid comprises 100 0.1mmX0.1mm square small grids; along the side frame of each big grid from the top to the bottom and from the left to the right, the small grids are marked with numbers from 0 to 9; the numbers and grid lines on the grid positioning sheet are all displayed via highlight black lines. The sperm grid positioning sheet is suitable for observing of sperm morphology under a high-power microscope; accurate positioning of the observed sperms can be realized via the big and the small grids which are marked with English letters and Arabic numerals; and it is extremely convenient to find target sperms when repeated checking on specific sperms is necessary.

Description

technical field [0001] The invention relates to the research of cell biology, in particular to the research of sperm morphological localization. Background technique [0002] Since the specimen must have a relatively high concentration when making a semen smear, there are often hundreds or thousands of sperm on a smear. The diameter of the sperm head is less than 6 μm and the shape varies greatly. Regardless of whether it is stained or not, it is difficult to repeat the inspection of the specific sperm that has been examined after the smear is dried. The current general-purpose sperm morphology smear cannot locate the attached fixed sperm, which is not conducive to the comparative observation and repeatable measurement of sperm morphology, making the preservation of sperm smears worthless for research. Contents of the invention [0003] The purpose of the present invention is to design a grid composed of several small square grids with a side length of 0.1mm on the polish...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCG01N1/2806G01N1/2813
Inventor 徐元诚商学军
Owner 徐元诚
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