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Application of pattern recognition receptors RIG-I in preparation of antitumor drug

A technology of pattern recognition receptors and anti-tumor drugs, applied in the fields of biotechnology and medical diagnosis

Inactive Publication Date: 2014-03-26
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] At present, there is no research report on the correlation between the pattern recognition receptor RIG-I and tumors at home and abroad.

Method used

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  • Application of pattern recognition receptors RIG-I in preparation of antitumor drug
  • Application of pattern recognition receptors RIG-I in preparation of antitumor drug
  • Application of pattern recognition receptors RIG-I in preparation of antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: the preparation of detection kit

[0086] The detection kit is prepared according to the following composition, and the kit is suitable for detecting the expression of RIG-I in a biological sample with real-time quantitative reverse transcription PCR (qRT-PCR) method:

[0087] (a) a container with reverse transcriptase (200U / μl);

[0088] (b) a container containing an RNase inhibitor (40 U / μl);

[0089] (c) Equipped with reverse transcription 5× buffer (75mM KCl, 500mM Tris-Cl, pH8.3, 25°C, 3mM MgCl 2 , 10mM DTT) container;

[0090] (d) A container containing PCR upstream primers and downstream primers (10 μM) of the target RIG-I molecule: the RIG-I reverse transcription primer is: Oligo (dT);

[0091] Quantitative PCR primers:

[0092] 5'-TGT GCT CCT ACA GGT TGT GGA-3' (upstream, SEQ ID NO: 1) and

[0093] 5'-CAC TGG GAT CTG ATT CGC AAA A-3' (downstream, SEQ ID NO: 2);

[0094] (e) A container containing internal reference reverse transcription prim...

Embodiment 2

[0103] Example 2: Correlation between RIG-I expression and patient survival time in HCC

[0104] The expression of RIG-I protein level in HCC tissues from two groups of 74 HCC control patients (not receiving IFN-α treatment) collected from 1999 to 2006 was analyzed by immunohistochemical method. According to the median expression of RIG-I, 74 HCC control patients were divided into two groups, 37 in each group. (Group 1 tissue sections are from Hong Kong University, group 2 tissue sections are from Shanghai Zhongshan Hospital)

[0105] The correlation between the low expression of RIG-I and the patient's survival time was analyzed respectively, and the P value was calculated using the log-rank test in SPSS17.0. The results are as follows figure 1 A and figure 1 Shown in B.

[0106] It was found that the low expression of RIG-I was significantly correlated with the lower disease-free survival time of patients ( figure 1 A).

[0107] It was found that the low expression of R...

Embodiment 3

[0117] Example 3: Correlation between the expression of RIG-I in HCC and the therapeutic effect of IFN-α in patients

[0118] The expression of RIG-I protein level in tumor tissues of 151 HCC patients (including control and IFN-α-treated patients) collected from 1999 to 2006 was analyzed by immunohistochemical method. Using the median expression of RIG-I as the cutoff, 76 HCC patients in group 1 and 75 HCC patients in group 2 were divided into high and low groups.

[0119] The correlation between the low expression of RIG-I and the survival time of patients was analyzed respectively, and the P value was calculated using the log-rank test in SPSS17.0. The results are as follows figure 2 A and figure 2 Shown in B. It was found that the low expression of RIG-I was significantly correlated with the lower overall survival time of patients ( figure 2 A, B).

[0120] Cox regression analysis was performed on the risk factors affecting the prognosis of HCC, and the hazard ratio ...

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Abstract

The invention relates to the field of biotechnology and medical diagnosis. According to the invention, molecule-pattern recognition receptors RIG-I with tumor expression correlation and having regulation effect on the growth of cancer cells are screened out from gene chip. The invention further provides application of the pattern recognition receptors RIG-I in preparation of anticancer drugs; the application specifically refers to combination between the pattern recognition receptors RIG-I and interferon alpha. The invention provides a new idea for oncotherapy.

Description

technical field [0001] The invention relates to the fields of biotechnology and medical diagnosis, in particular to the application of pattern recognition receptor RIG-I in the preparation of antitumor drugs. Background technique [0002] In the process of pathogenic organisms infecting the body, the body's innate immune response is first activated. The activation of innate immune response mainly depends on the recognition of pathogen-specific components by pattern recognition receptors (PRRs) on the surface of immune cells. The PRRs discovered so far are mainly divided into three categories: 1. TLRs (Toll-like receptors); 2. RLHs (RIG-I-like helicases); 3. NLRs (Nucleotide-oligomerization domain-like receptors). In the process of antiviral innate immune response, viral components such as DNA or RNA can be recognized by TLRs and RLHs of immune cells, thereby activating downstream signaling pathways, prompting immune cells to activate and express pro-inflammatory cytokines a...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K38/21A61K38/00A61P35/00A61P1/16
Inventor 侯晋曹雪涛周烨
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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