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Bacillus sp.N11-8 and active component thereof having antitumor action

A marine bacillus, anti-tumor activity technology, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, bacteria, etc., can solve the problems of low content, difficulty in large-scale preparation, difficult drug sources, etc., to achieve good research and Application value, good acid-base and thermal stability, low cytotoxic effect

Active Publication Date: 2015-04-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the vast majority of anti-tumor lead compounds have low content in the body, and it is difficult to prepare them on a large scale. Therefore, the source of anti-tumor lead compounds from natural sources is a big problem at present.

Method used

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  • Bacillus sp.N11-8 and active component thereof having antitumor action
  • Bacillus sp.N11-8 and active component thereof having antitumor action
  • Bacillus sp.N11-8 and active component thereof having antitumor action

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation of strains and primary screening of activity

[0029] Chase's medium Cha (for cultivating mold), seawater basal medium 2216E, beef extract peptone medium NRG (for cultivating bacteria), Gao's No. 1 medium G1 (for cultivating actinomycetes) and Martin's medium MD (for cultivating Fungi) and other different media were used to selectively isolate and cultivate Antarctic seawater samples, and a total of 50 single bacteria were obtained. Preliminary screening of the anti-tumor activity of these 50 microbial fermentation products showed that one strain had an inhibition rate of more than 60% against liver cancer cell BEL-7402, and the proportion of active strains was 2%; 2 strains; 5 strains with an inhibition rate of more than 30%; the remaining strains have almost no inhibitory effect on tumor cells.

Embodiment 2

[0031] Inhibitory Effects of Bacterial Strains on Different Tumor Cells

[0032] The three active strains with an inhibition rate of more than 50% were screened again, using human colon cancer cell HCT-116, human breast cancer cell MCF-7, pancreatic cancer cell P28, human non-small cell lung cancer cell A549 and human glioma Six strains of tumor cells such as cell U251 were used as targets, and the SRB method was used for multi-target screening, and it was found that the fermentation broth of Antarctic bacteria N11-8 (No. All of them have good proliferation inhibitory effect, and the inhibition rate of MCF-7 is 76.2%. As in Table 1:

[0033] Table 1 Inhibition rate of Antarctic bacteria N11-8 on different tumor cells

[0034]

Embodiment 3

[0036] Morphological and Physiological and Biochemical Characteristics of Strain N11-8

[0037] (1) Morphological characteristics

[0038]Gram staining results showed that the strain N11-8 was a Gram-negative bacteria. Strain N11-8 has two important characteristics of Bacillus, which are bacillus morphology and spore-forming ability. It is 0.5-2.5 μm long, 0.5-0.8 μm wide, and oval spores are terminal ( figure 1 ). After being cultured on beef extract peptone NRG medium for 24 hours at 25°C, the colonies are round, entire, flat, with a diameter of 0.8 to 2.1 mm, smooth and shiny, with a mucus-like appearance, creamy texture, and white translucent.

[0039] The bacterium is an aerobic bacterium and grows well when the pH value is 7.5, the culture temperature is 25°C, and the medium contains 6% (w / v) NaCl.

[0040] (2) Physiological and biochemical reaction characteristics

[0041] Mainly according to the gold standard for bacterial identification (API), use the API20E and A...

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Abstract

The invention relates to a Bacillus sp.N11-8 and an active component thereof having antitumor action, belonging to the technical field of marine microorganism medicine. The Bacillus sp.N11-8 is obtained by isolated culture in Antarctic Ocean water, and was collected at China Center for Type Culture Collection (Wuhan) on November 19, 2012; and the collection number is CCTCC NO:M2012459. The strain can stably express the active component having antitumor action. The active component has favorable acid / alkali stability and heat stability, and has bigger cytotoxic effect on multiple tumor cells and smaller cytotoxic effect on normal cells of humans. Thus, the Bacillus sp.N11-8 and the active component thereof have favorable research and application values.

Description

technical field [0001] The invention belongs to the technical field of marine microbial medicine, and in particular relates to a marine bacillus and its active component with antitumor effect. Background technique [0002] Malignant tumor is a major disease that seriously endangers human health and is one of the main causes of population death in my country. In 2005, about 7.5 million people died of malignant tumors in the world, and about 1.3 million people died of cancer in my country every year, and the incidence rate is increasing year by year. Upward trend. Drug therapy is an important part of comprehensive therapy for malignant tumors. Classical cytotoxic antineoplastic drugs are the main means of chemotherapy at present, and will still occupy a dominant position for a long time in the future. Therefore, looking for anti-tumor active ingredients with new structure types or new mechanisms of action from natural organisms is a new trend in current anti-tumor drug researc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A61K35/742A61P35/00C12R1/07
Inventor 郑兰红孙谧陈世建衣尧郑媛王伟盛军纪晓峰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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