35 results about "Bacillus oceanisediminis" patented technology

The invention provides a culture medium for producing spores, and the optimizing process and application thereof, which are particularly applied to marine bacillus which hardly generates spores in a common liquid medium. The culture medium comprises the following components in percentage by weight: 0.5 to 3 percent of cornmeal, 0.1 to 1 percent of bran, 0.1 to 0.5 percent of soybean cake meal, 0.01 to 0.1 percent of potassium dihydrogen phosphate dodecahydrate, 0.05 to 0.3 percent of calcium carbonate anhydrous and the balance of water. The culture medium has the advantages of readily available raw materials, low cost and simple preparation, and can obviously improve the spore formation rate and total active spores of marine bacillus 9912 and marine bacillus subtilis 3728. By the culture medium and the application of the culture medium, essential conditions are provided for developing bacillus licheniformis 9912 active spore preparations and other related active spore preparations.

The invention belongs to the field of biotechnology, and discloses a marine bacillus (Bacillus sp.BS176) and application of a lipopeptid molecule of the marine bacillus. The marine bacillus for generating the lipopeptid is Bacillus sp.BS176, the bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the address of NO.3, 1# yard, West Beichen Road, Chaoyang District, Beijing (Institute of Microbiology, Chinese Academy of Sciences), a reservation date is December, 2016, and a reservation number is CGMCC No.13515. The bacillus is separated from the western pacific seamount area and belongs to a marine microorganism. The lipopeptid generated by the marine bacillus has the functions of inhibiting the movement ability of aquaculture pathogenic bacteria and accelerating somatic agglutination and sedimentation for various pathogenic bacteria. The bacillus and the lipopeptid generated by the bacillus have potential application values on the aspects of the development of broad-spectrum antibacterial medicines, the improvement of aquaculture environment and the like.

The invention relates to a separation and purification method of high yield and low-temperature catalase of Antarctic marine Bacillus sp n2a. The invention can achieve electrophoretic pure low-temperature catalase through the processing of strains shaking culture, the ultrasonication ammonium sulfate precipitation, anion-exchange chromatography and gel filtration. With simple processing art, and fast and good effect, the invention can be used for separating and purifying low-temperature catalase in effective, reliable and economical way.

The invention discloses a marine bacillus polypeptide and a preparation and an application thereof and belongs to the technical field of marine microorganism medicines. The marine bacillus polypeptide is from metabolites of antarctic marine bacillus N11-8 subjected to fermentation cultivation; 15 amino acid sequences at N-ends of the active polypeptide are ASTGSQKVTVYAVAD; the active polypeptide has relatively high cytotoxicity to a plurality of tumor cells such as human hepatoma carcinoma cell BEL-7402, human ovarian carcinoma cells NIH:OVCAR-3, human renal clear cell carcinoma cells 786-0 and human large cell lung cancer cells NCI-H460, and has relatively good research and application value. The polypeptide can be applied to medicines for preventing and / or treating the cancers.

The invention discloses a method of controlling pepper bacterial wilt. The pepper bacterial wilt is prevented and controlled through soil improvement, soil nutrient balancing, and a seedling stage resistance induction processing system; To be specific, soil amendment is added to cultivation land for peppers and then deep tillage on soil is performed before winter; then special fertilizer is applied in the improved soil and hole application of special fertilizer is performed before seedling stage transplantation; pepper roots are dipped in bacteria liquid of Paenibacillus polymyxa or marine bacillus before transplantation; then root-irrigation processing is performed with mixed solution of zhongshengmycin and thiediazole copper at early blooming period for 2 to 3 times and every 7 to 10 days each time. The use of the method disclosed in the present invention can delay the outbreak of pepper bacterial wilt and increase the yield of pepper, improve the quality of pepper and improve the soil, and can be widely used in pepper planting industry.

The invention relates to an ocean bacillus protein having antitumor activity and preparation and application thereof and belongs to the field of genetic engineering and biological medicines. The nucleotide sequence of the protein is as shown SEQ ID No. 1; the sequence can encode the ocean bacillus protein, and the amino acid sequence of the protein is as shown SEQ ID No. 2. The ocean bacillus protein can inhibit growth of various tumor cells and have the potential of being developed into antitumor drugs and / or healthcare products.

The invention relates to a beta-glucanase generated by Bacillus marinus and a preparation method thereof, belonging to the fields of marine microbes and enzyme preparations. The beta-glucanase is generated by Bacillus marinus N6-2, and the molecular weight is 24kDa. The beta-glucanase has the advantages of higher enzyme activity, favorable heat stability and favorable pH stability, and has wide development potential and application prospects in the aspects of wine industry, industrial wastewater treatment, functional food development and feed application.

The present invention discloses a bacillus marinus with multiple plant functions, bacillus marinus BY-1188 strain is obtained from marine living condition, artificial mutation breeding and optimization of culture conditions are performed, the accession number of the BY-1188 strain is CGMCC No. 6187, and through culture optimization tests of the strain, the optimum fermentation broth formula is as follows: 2-5g of peptone, 1-3g of yeast powder, 25-30g of NaCl, 0.3-0.5g of KCl, 1.9-2.5g of MgCl2 . 7H2O, 0.01-0.02g of FePO4 and 1000ml of water, pH is 7.0-7.5, the culture medium is added with 16-19g of agar to be used as a solid medium. The bacillus marinus has the advantages of biological characteristics and stable agrochemical activity, and has no environmental pollution.

The invention relates to an ocean source catalase high-yielding strain and a method for producing catalase by using the strain. One strain of Oceanobacillus iheyensis is obtained by directionally enriching and sieving from an ocean water body through adopting a semi-solid culture medium; and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 6042. The strain is fermented to obtain the catalase and the enzyme activity reaches 8677U / mg. A preparation process of the catalase is simple; the enzymatic activity is high and the cost is low; and the catalase is suitable for being widely applied to the fields of foods, spinning, papermaking, environmental friendliness and the like. Therefore, an enzyme producing strain and a fermentation method disclosed by the invention have wide industrial application values and obvious economic benefit prospects.

The invention relates to a bacillus marinus S-1 and an anti-tumor active polypeptide produced thereby. The bacillus marinus S-1 is cultivated, separated and screened from sea mud in the south china sea, so as to obtain a marine microbial bacteria strain of marine bacillus genus (Bacillus sp.) for producing the anti-tumor active polypeptide. The strain S-1 is aerobic bacteria, has good salt tolerance, and grows well in 7 w / v% of NaCl culture solution, wherein the growth pH range is 5.0-9.0; the growth temperature range is wide; the bacillus marinus S-1 can well grow in the environment of 15-37 DEG C; a gene sequence of 16S rDNA of the strain S-1 is shown in a figure. The polypeptide produced by the bacillus marinus S-1 has anti-tumor activity, and is expected to be an anti-tumor drug for treating a liver cancer.

The invention relates to the technical field of flocculating agent preparation, and solves problems of low polysaccharide yield and low polysaccharide flocculation activity in an existing microorganism. The invention discloses a method for preparing a flocculating agent by using Bacillus marinus. The method for preparing the flocculating agent by using the Bacillus marinus comprises the followingsteps: firstly, inoculating a Bacillus marinus strain into a solid culture medium and adding sterile water to the solid culture medium to prepare a strain liquid; secondly, inoculating the strain liquid into a liquid culture medium according to an inoculating quantity to ferment and culture the strain liquid so as to obtain a fermentation liquor; thirdly, adding a flocculation carrier to the fermentation liquor to continue to ferment the fermentation liquor so as to obtain a raw material liquor; and centrifugally separating and drying the raw material liquor to obtain an active polysaccharideflocculating agent. The method for preparing the flocculating agent by using the Bacillus marinus, disclosed by thei nvention, has the advantages that the yield of the active polysaccharide produced by using the Bacillus marinus strains is higher, and the flocculation activity of the active polysaccharide produced by using the Bacillus marinus strains is also higher.

The invention belongs to the technical field of biological medicines, and particularly relates to bacillus marinus and application thereof. The bacillus marinus is a polysaccharide producing marine bacterium Bacillus sp. BS11; a strain is applied to the preparation of an anti-cancer drug, an anti-tumor metastasis drug, an anti-cancer health product or an anti-cancer recurrence health product; thebacillus marinus provided by the invention is separated from the seamount and can be used for preparing the anti-cancer drug or the anti-cancer health product or preparing the anti-tumor metastasis drug or an anti-tumor formation health product. The invention discloses application of an extracellular polysaccharide of a bacillus to the preparation of anti-cancer and anti-tumor metastasis drugs andhealth products, and the bacillus marinus can be used for inhibiting the expression, in a cell A549, of betaIII-tubulin, is used for inhibiting the formation of byssus on the surface of a cancer cell, inhibiting the adherence and the attachment of the cell A549 and inhibiting the metastasis of the cell A549, is finally used for causing the anoikis of the cell, and therefore, has certain prevention and treatment effects of malignant tumor.

The invention discloses a seed coating agent capable of resisting the corn stalk rot diseases and improving the seed vigor. The seed coating agent is prepared from the following ingredients in parts by weight: 3 to 5 parts of bacillus marinus, 0.5 to 1 part of validamycin, 5 to 7 parts of plant bacteriostatic agents, 6 to 10 parts of kieselguhr, 1 to 2 parts of humic acid, 1 to 2 parts of chelatedfertilizers, 0.03 to 0.06 part of dispersing agents, 1 to 2 parts of preservatives and 3 to 5 parts of film formers. The corn seed coating agent can be used for effectively preventing and treating corn stalk rot diseases, effectively supplementing trace elements such as molybdenum, ferrum and boron, regulating the growth and development of corn, improving the soil fertilizer utilization rate andimproving the yield.

The invention discloses a compound pesticide used for controlling paddy rice sheath blight. The invention belongs to the technical field of compound pesticides. The compound pesticide comprises bacillus marinus and jingganmycin, wherein a weight ratio of bacillus marinus to jingganmycin is 1:0.0005-500. According to the invention, because the two biological pesticides are different in action mechanisms, through the compounding of the two, a synergetic effect is developed, pesticide dosage is reduced, controlling effect is improved, controlling time is prolonged, and the occurrences of drug resistance are reduced.

The invention discloses a bacillus marinus bacterium agent as well as a preparation method and application thereof. The bacterium agent is prepared from bacillus marinus bacterium powder and humic acid, wherein the bacillus marinus bacterium powder is obtained by fermenting bacillus marinus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 13962 on a fermentation culture medium. The bacillus marinus bacterium agent has the beneficial effects that (1) the soil fertility can be improved and the growth of plants is promoted; a test verifies that the bacillus marinus bacterium agent disclosed by the invention can be used for remarkably promoting the growth of the plants and is adaptive to various crops; (2) the preparation method is simple and efficient and is suitable for industrial production.

The invention discloses an organic fertilizer which is prepared from olive waste material and is used for improving olive orchard soil. The organic fertilizer which is prepared from olive waste material and is used for improving olive orchard soil comprises, by weight, olive fruit residue, olive leaf, olive branch pyroligneous liquor, bran powder, a biological nutritional agent, polyacrylamide, marine bacillus, Bacillus thuringiensis, phanerochaete chrysosporium, rape seed cake, modified biochar, polyvinyl acetate, corn stalk, brassinolide, and ammonium sulfate. The biological nutritional agent is composed of extract yeast, cane sugar, peptone, potassium acetate, and sodium phosphate at a weight ratio of 4:3:2:1:1. According to a preparation method, full using of olive fruit residue and fruit leaf is adopted for production of the organic fertilizer, soil conditions can be improved effectively, soil pH value adjusting is realized, microecology adjusting and granular structure adjustingare realized, immobilized nutrient element activity adjusting is realized, and absorption removing of soil harmful metal element effect is achieved.

The invention relates to a marine bacillus and its polypeptide with antitumor activity. The strain is preserved at China Center for Type Culture Collection in Wuhan, and its preservation number is CCTCC M 2013063. A novel marine bacillus polypeptide with anti-tumor activity is obtained from a fermentation product of the strain. By means of an MTT method, the invention finds that the polypeptide has an obvious proliferation inhibition effect on human hepatoma carcinoma cell BEL-7402, human breast cancer cell MCF-7, human glioma cell U251, human non-small-cell lung cancer cell A549 and other tumor cells, has relatively small cytotoxicity on human fibroblast HFL1, and can be applied in drugs treating human liver cancer, gliomas, lung cancer and breast cancer.

The invention discloses a multi-effect soil conditioner for improving land capability decline of olive timbered soil by using olive wastes. The multi-effect soil conditioner is prepared from the following raw materials in parts by weight: olive pomace, olive leaves, olive branch pyroligneous, phenolic resin, polymethyl methacrylate, oyster shell powder, polyvinyl alcohol, a biological nutrient, polyacrylamide, bacillus marinus, bacillus thuringiensis, phanerochaete chrysosporium, rape seed cakes, modified charcoal, polyvinyl acetate, maize straws, propionyl brassinolide and ammonium sulfate. The biological nutrient is prepared from yeast extract, cane sugar, peptone, potassium acetate and sodium phosphate in a ratio of 4: 3: 2: 1: 1. Wastes such as the olive pomace and olive leaves are fully used, soil is improved by a double regulating mode biologically and chemically by using a mixed material of biological, organic, inorganic and high molecular materials, the land capability declineof olive timbered soil is improved, the productivity of the olive timbered soil is improved, and the output of olive is improved.

The invention discloses a bacillus marinus containing sterilization composition and application thereof. The sterilization composition contains an active component A and an active component B, whereinthe active component A is bacillus marinus B-9987, and the active component B is any one of the following bactericides: cyprodinil, fludioxonil and diethofencarb; the weight ratio of the active component A to the active component B is (1 to 80) to (1 to 80). The sterilization composition can be used for preventing and controlling a plurality of bacterial and fungal diseases of fruit trees, vegetables and cereal crops, realizes an obvious synergistic effect, delays drug resistance of the pathogenic bacteria, has long duration, reduces the frequency of drug administration as well as the dosage,and lowers the using cost. The sterilization composition disclosed by the invention can be used for preventing and treating the diseases such as gray mold of tomatoes, grapes and cucumbers while ensuring a remarkable effect.

The invention discloses an olive waste material organic fertilizer, which is prepared from the following ingredients in parts by weight: olive fruit residue, olive leaves, olive branch wood vinegar liquid, rice bran powder, mushroom residue, polyacrylamide, bacillus marinus, bacillus thuringiensis, phanerochaete chrysosporium, rape seed cake, modified biochar, polyvinyl acetate, Epsilon-polylysine, propionyl brassinolide and ammonium molybdate. The organic fertilizer is produced by sufficiently utilizing olive fruit residue and olive leaves; the organic fertilizer has rich nutrition and high fertilizer effect; the fertilizer is favorable for the growth and development of the crop root systems; the soil conditions can be effectively improved, so that the soil becomes loose and fertile; thefertilizer is favorable for the cultivation and the crop root system growth and development; the production increase is promoted; the good regulation and improvement effects are achieved on the soil;various effects of soil PH value regulation, micro-ecology regulation, aggregate structure regulation, fixed nutrition element activity regulation, soil harmful metal element adsorption removal and passivation and the like are achieved.

The invention discloses a preparation method of hairtail active extract for repairing bone joints; the preparation method comprises the steps of enzyme complex hydrolysis, multistage membrane filtration, nanofiltration, HPLC (high-performance liquid chromatography) eluting and lyophilizing; the step of enzyme complex hydrolysis includes enzymatically hydrolyzing hairtail flesh homogenate via Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase, and performing ultrasonic-assisted enzymatic hydrolysis. The preparation method has the advantages that marine organism proteins are greatly differed from terrestrial life proteins in both amino acid composition and amino acid sequence and hairtail proteins can be enzymatically hydrolyzed via the Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase to obtain mass active peptides; the obtained hairtail active extract can promote the generation of cartilage cells, joint synovial fluid can be massively induced and supplemented, the generation of collagen matrix can be effectively promoted, regeneration of connective tissue cells is stimulated, and the reconstruction and recovery of soft tissues are accelerated accordingly.

The invention relates to the technical field of bio-fertilizers, especially to a novel composite microbial fertilizer for vegetable pests and diseases control. The novel composite microbial fertilizer for vegetable pests and diseases control comprises the following ingredients (by weight): 7-13 parts of bacillus methylotrophilus, 27-35 parts of bacillus subtilis (CD-2), 3-9 parts of bacillus marinus, 100-300 parts of sterilized straw, 50-70 parts of cellulose hydrolysate, 10-15 parts of animal bone meal, 500-750 parts of sterilized furfural residue and 200-350 parts of dextrin. The invention has the following beneficial effects: the fertilizer has balanced nutrients and high content of live bacteria, is easy to store and has a good yield-increasing effect.

The invention discloses a hairtail activity extract for articulation restoration. The hairtail activity extract is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC (High Performance Liquid Chromatography) elution and freeze drying, wherein the compound enzyme enzymolysis comprises the steps of performing enzymolysis on hairtail meat homogenate by adopting marine bacillus produced alkaline protease and porphyra yezoensis protease, and performing ultrasonic for assisting in the enzymolysis. The hairtail activity extract has the benefits that the composition and a sequence of amino acid of marine organism protein are very different from those of terrestrial organism protein; when the marine bacillus produced alkaline protease and the porphyra yezoensis protease simultaneously perform the enzymolysis on hairtail protein, abundant activated peptide fragments can be obtained; and the obtained hairtail activity extract can promote generation of cartilage cells, can greatly hasten and supplement articulation synovia, can effectively promote generation of collagen stroma, stimulates regeneration of connective tissue cells, and further accelerates reconstruction and recovery of a soft tissue.

The invention discloses an improved biological preservation agent for aquatic products. The preservation agent is prepared from the following components in percentage by weight: 0.1-1 percent of glucose oxidase, 1-3 percent of tea polyphenol, 1-3 percent of water-soluble chitosan and the balance of water, wherein the glucose oxidase is prepared from bacillus marinus which is separated from deep sea by the laboratory. Compared with current commercial glucose oxidase preparations, the biological preservation agent has the advantage of higher activity at low temperature, and is more suitable for quality preservation in the aquatic product cold chain logistic process. The preservation agent is remarkable in preservation effect, can be used for remarkably inhibiting decaying or decomposition caused by blackening and bacteria of aquatic products and prolonging shelf lives. The preservation agent is low in cost and simple in operation, and is convenient and practical.

The invention discloses a compound pesticide used for controlling paddy rice sheath blight. The invention belongs to the technical field of compound pesticides. The compound pesticide comprises bacillus marinus and jingganmycin, wherein a weight ratio of bacillus marinus to jingganmycin is 1:0.0005-500. According to the invention, because the two biological pesticides are different in action mechanisms, through the compounding of the two, a synergetic effect is developed, pesticide dosage is reduced, controlling effect is improved, controlling time is prolonged, and the occurrences of drug resistance are reduced.

The invention discloses a novel composite probiotic polysaccharide gum promoting penaeus vannamei growth and application thereof. A novel composite probiotic polysaccharide gum aqueous solution promoting penaeus vannamei growth is prepared by: mixing Bacillus marinus raw powder, bacteria swallowing vibrio raw powder, carrageenan, xanthan gum, guar gum, konjac gum and Sa-son seed gum evenly, and dissolving the mixture with water into a solution. The process of applying the novel composite probiotic polysaccharide gum to prepare feed containing the novel composite probiotic polysaccharide gum consists of: mixing the novel composite probiotic polysaccharide gum aqueous solution with basic feed evenly, and performing airing in a shade place at room temperature. Compared with traditional antibiotics, the novel composite probiotic polysaccharide gum promoting penaeus vannamei growth provided by the invention is safer, cannot cause serious drug resistance and drug residue to the penaeus vannamei, has more significant promoting effect on penaeus vannamei growth, and is a new product able to effectively promote penaeus vannamei growth and quality safety.

The present invention relates to Bacillus marinus-pyrimethanil compounded bactericide and applications thereof, particularly to a compounded bactericide containing Bacillus marinus and pyrimethanil, a pesticide and a plant disease prevention and control method, wherein a weight ratio of Bacillus marinus to pyrimethanil is 1:0.0005-500, and the compounded bactericide or pesticide can be especially used for preventing and controlling plant gray mold. According to the present invention, the action mechanisms of the two pesticides are different, and the two pesticides are compounded so as to provide the synergetic effect of the microorganism and the chemical agent, and achieve the purposes of advantage complementation, prevention effect improving, chemical agent consumption reducing, reduction of chemical agent residue in vegetables, fruits and flowers, and drug resistance incidence reducing.