Bacillus marinus containing sterilization composition and application thereof

A technology of marine bacillus and composition, applied in the application, fungicides, biocides and other directions, can solve the problem of difficult to completely eliminate pathogenic bacteria, and achieve the effect of delaying the generation of pest resistance, good synergy, and less dosage

Inactive Publication Date: 2018-12-21
江西正邦作物保护股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Botrytis cinerea is a typical airborne disease. In recent years, the incidence of crops has shown an obvious upward trend. In the actual disease control process, it is difficult to take effective measures to completely cut off the source of infection. It is also difficult to completely eradicate pathogenic bacteria

Method used

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  • Bacillus marinus containing sterilization composition and application thereof
  • Bacillus marinus containing sterilization composition and application thereof
  • Bacillus marinus containing sterilization composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Indoor Toxicity Determination of Marine Bacillus B-9987 and Cyprodinil Compounded to Tomato Botrytis Botrytis

[0028] Determination method of indoor antibacterial effect: The growth rate method is used to determine the inhibitory effect of the fungicidal composition on Botrytis cinerea. Take 5mm-diameter mycelium blocks from the edge of wheat take-all colonies cultured on PDA medium for 4 days and inoculate them on PDA medium plates containing different concentrations of fungicides, culture at a constant temperature of 28°C, and use the plates without agents as blank As a control, measure the colony diameter of each treatment after 4 days, and calculate the inhibitory percentage of the medicament to mycelial growth. Regression analysis was carried out by the logarithm of each treatment dose and the probability value of the mycelial growth inhibition rate, and the inhibitory medium concentration (EC) of the treatment fungicide to tomato gray mold disease was c...

Embodiment 2

[0036] Example 2 Indoor virulence determination of marine bacillus B-9987 and fludioxate compounded against botrytis cinerea

[0037] Indoor bacteriostatic effect test method: the growth rate method was used to measure the inhibitory effect of the bactericidal composition on botrytis cinerea. Take mycelia pieces with a diameter of 5 mm from the edge of wheat sheath blight colonies cultured on PDA medium for 4 days and inoculate them on PDA medium plates containing different concentrations of fungicides, culture at a constant temperature at 28°C, and use the plates without agents as blank As a control, measure the colony diameter of each treatment after 4 days, and calculate the inhibitory percentage of the medicament to mycelial growth. The logarithm of each treatment dose and the probability value of the mycelial growth inhibition rate were used for regression analysis to calculate the inhibitory medium concentration (EC 50 ), and then calculate the co-toxicity coefficient (...

Embodiment 3

[0046] Example 3 Indoor Toxicity Determination of Cucumber Botrytis in Combination of Marine Bacillus B-9987 and Ditofencarb

[0047] Determination method of indoor antibacterial effect: The growth rate method is used to determine the inhibitory effect of the fungicidal composition on Botrytis cinerea. Take mycelium blocks with a diameter of 5 mm from the edge of the wheat root rot colony cultured on PDA medium for 4 days and inoculate them on PDA medium plates containing different concentrations of fungicides, and culture them at a constant temperature at 28°C. As a control, measure the colony diameter of each treatment after 4 days, and calculate the inhibitory percentage of the medicament to mycelial growth. Through the regression analysis of the logarithm of each treatment dose and the probability value of mycelial growth inhibition rate, the inhibitory medium concentration (EC) of fungicides to cucumber gray mold was calculated. 50 ), and then calculate the co-toxicity c...

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Abstract

The invention discloses a bacillus marinus containing sterilization composition and application thereof. The sterilization composition contains an active component A and an active component B, whereinthe active component A is bacillus marinus B-9987, and the active component B is any one of the following bactericides: cyprodinil, fludioxonil and diethofencarb; the weight ratio of the active component A to the active component B is (1 to 80) to (1 to 80). The sterilization composition can be used for preventing and controlling a plurality of bacterial and fungal diseases of fruit trees, vegetables and cereal crops, realizes an obvious synergistic effect, delays drug resistance of the pathogenic bacteria, has long duration, reduces the frequency of drug administration as well as the dosage,and lowers the using cost. The sterilization composition disclosed by the invention can be used for preventing and treating the diseases such as gray mold of tomatoes, grapes and cucumbers while ensuring a remarkable effect.

Description

technical field [0001] The invention relates to the field of agricultural disease prevention and control, in particular to a fungicidal composition containing marine bacillus and application thereof. Background technique [0002] Bacillus marinus is a group of facultative anaerobic, spore-forming, Gram-positive rod-shaped bacteria belonging to the genus Paenibacillus. At present, no research has shown that marine bacillus can cause human, animal or plant pathogenicity, that is, the bacteria is safe for humans and animals, and can be used to develop microbial pesticides. [0003] The marine Bacillus B-9987 strain obtained by East China University of Science and Technology and Shanghai Zeyuan Marine Biotechnology Co., Ltd. comes from the root of Suaeda salsa, a plant in the intertidal zone of Bohai Sea, and is effective against various pathogenic fungi such as gray mold and bacterial wilt. have a good inhibitory effect. The marine bacillus B-9987 strain was identified and pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N47/20A01N43/54A01N43/36A01P3/00
CPCA01N43/36A01N43/54A01N47/20A01N63/10
Inventor 李思源谢远芳代新红周蕲李扬
Owner 江西正邦作物保护股份有限公司
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