An improved biological preservative for aquatic products, its preparation method and application

A technology of biological preservatives and aquatic products, applied in the direction of preserving meat/fish with chemicals, which can solve the problems of low enzyme production activity and complicated separation, and achieve the effects of simple operation, good sensory perception, and inhibition of spoilage

Active Publication Date: 2018-06-22
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glucose oxidase is an enzyme worthy of attention. However, the commercialized glucose oxidase at this stage is produced by mold fermentation, which has the defects of low enzyme production activity and complicated separation. Therefore, the separation and purification of enzyme with high enzyme production activity is relatively simple. The target microorganism is the key to reduce the cost of enzyme preparation

Method used

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  • An improved biological preservative for aquatic products, its preparation method and application
  • An improved biological preservative for aquatic products, its preparation method and application
  • An improved biological preservative for aquatic products, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the screening of producing glucose oxidase bacterial strain

[0038] The screening method of bacillus CAMT22370 of the present invention comprises the following steps:

[0039] (1) Enrichment of bacteria: Weigh 1g of sea mud and shake it evenly in a 9ml sterile seawater test tube, let it stand still, take the supernatant and dilute to 10 -2 , 10 -3 , l0 -4 After oscillating evenly, take 1 mL of sample solutions of different concentrations and inoculate them into each culture medium, and place them in an incubator at 30°C for upside-down cultivation.

[0040] (2) Primary screening: Dilute the enriched bacteria to about 10 4 / mL concentration, according to the product H 2 o 2 Methylene blue can be oxidized to colorless, and a chromogenic medium containing a concentration gradient of methylene blue is designed. The diluted bacterial suspension is inoculated on the chromogenic medium by the dilution coating method, and after culturing at 30°C for 48 hours...

Embodiment 2

[0042] Embodiment 2: Identification of producing glucose oxidase strain

[0043] The strains screened in Example 1 were identified by morphological and molecular biological methods.

[0044] The morphological identification mainly adopts the observation of colony morphology and microscopic morphology, and the physiological and biochemical identification adopts glycolysis test, starch hydrolysis test, V-P test, methyl red test, oxidase test, three sugar iron agar test and sulfuration test according to the metabolic process of the strain. The method of hydrogen indigo matrix-dynamic agar test was carried out, and the identification of molecular biology was carried out by 16SrDNA sequencing and comparison. The strains were assigned based on the results of morphological, physiological, biochemical and biomolecular identifications.

[0045] Among them, molecular biology identification firstly uses bacterial DNA extraction kit to extract genomic DNA, and then uses universal primers...

Embodiment 3

[0048] Embodiment 3: Bacillus CAMT22370 liquid fermentation produces glucose oxidase condition optimization

[0049] In this experiment, the enzyme production conditions of the screened Bacillus CAMT22370 were optimized. Specifically, it is mainly to optimize the culture medium and culture conditions for the production of glucose oxidase by liquid fermentation. Unless otherwise specified, the following percentages are percentages by weight.

[0050] 1. Screening of carbon source types: On the basis of 0.3% beef extract and 0.5% sodium chloride, glucose, lactose, sucrose, maltose, sorbitol, and mannose were added in an amount of 1% to make a carbon source containing only a certain carbon source. One carbon source medium. Fermentation inoculum volume is 5% (volume ratio), rotation speed 150rpm, 28 ℃ constant temperature fermentation culture for 72 hours, enzyme activity is measured, and the enzyme activity is used as the judgment standard. The carbon source with the largest en...

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PUM

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Abstract

The invention discloses an improved biological preservation agent for aquatic products. The preservation agent is prepared from the following components in percentage by weight: 0.1-1 percent of glucose oxidase, 1-3 percent of tea polyphenol, 1-3 percent of water-soluble chitosan and the balance of water, wherein the glucose oxidase is prepared from bacillus marinus which is separated from deep sea by the laboratory. Compared with current commercial glucose oxidase preparations, the biological preservation agent has the advantage of higher activity at low temperature, and is more suitable for quality preservation in the aquatic product cold chain logistic process. The preservation agent is remarkable in preservation effect, can be used for remarkably inhibiting decaying or decomposition caused by blackening and bacteria of aquatic products and prolonging shelf lives. The preservation agent is low in cost and simple in operation, and is convenient and practical.

Description

technical field [0001] The invention relates to the field of storage and processing of aquatic products, in particular to a composite biological antistaling agent for slowing down the quality deterioration of aquatic products during refrigeration. Background technique [0002] Vannamei( Litopenaeus vannamei ) as the shrimp species with the highest single yield among the world's three major cultured shrimps accounts for 70% to 80% of the total shrimp production in my country. However, due to its soft muscle tissue and strong cathepsin activity, the shrimp body turns black during the cold chain process. The quality deterioration represented by excessive microbes and microorganisms is relatively fast, which seriously affects the sensory and market value of prawns. [0003] Based on the blackening caused by the activation of endogenous polyphenol oxidase, and the spoilage and deterioration caused by the reproduction of endogenous and exogenous microorganisms, the main technical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B4/20A23B4/22
Inventor 徐德峰叶日英孙力军王雅玲伍彬廖建萌
Owner GUANGDONG OCEAN UNIVERSITY
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