Isolation and purification method for high-yield of low-temperature catalase by antarctic marine bacillus n2a

Abstract

The invention relates to a separation and purification method of high yield and low-temperature catalase of Antarctic marine Bacillus sp n2a. The invention can achieve electrophoretic pure low-temperature catalase through the processing of strains shaking culture, the ultrasonication ammonium sulfate precipitation, anion-exchange chromatography and gel filtration. With simple processing art, and fast and good effect, the invention can be used for separating and purifying low-temperature catalase in effective, reliable and economical way.

Description

technical field [0001] The invention relates to the field of marine microbial enzymes, in particular to a method for separating and purifying high-yield low-temperature catalase from Antarctic marine bacillus n2a. Background technique [0002] There are many kinds of low-temperature microorganisms living in the low-temperature environments of high mountains, deep seas and poles on the earth. They have evolved a complete set of adaptation mechanisms for survival and reproduction at around 0°C. Low temperature greatly reduces the rate of chemical reactions. How do low-temperature microorganisms maintain the normal progress of important biochemical reactions? , Enzymes that maintain normal activity at low temperatures are the key to ensuring the normal metabolism of low-temperature microorganisms. Low-temperature enzymes that catalyze important reactions are an important part of the study on the mechanism of low-temperature microorganisms adapting to low temperature. Generally...

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Problems solved by technology

Yumoto et al. first reported the properties of the low-temperature catalase (VRC) of the facultative psychrophilic bacteria Vibrio rumoiensis, and Lorentzen et al. studied the low-temperature catalase (VSC) from the fish pathogen Vibrio salmonicida, which made people preliminary Knowing low-temperature catalase, there are many differences between these two low-temperature enzymes and common low-temperature enzymes: the activity of VRC is much higher than that of bovine liver catalase and other medium-temperature enzymes at 40°C; the Km of VSC is at low temperature and medium temperature conditions are all lower than the catalase (PMC) of Proteus mirabilis; the separation and purification method to obtain VSC and VRC simultaneously is complicated, the steps are long, and the effect is not good enough, which cannot meet the needs of modern industry

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