Bacillus marinus and application thereof

The technology of marine bacillus and bacillus, which is applied in the field of biomedicine, can solve the problems of reducing the quality of life of cancer patients, specifically targeting tumor cell division obstacles, etc., and achieve the effect of malignant tumor prevention and treatment

Active Publication Date: 2018-05-04
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current drug candidates targeting β-tubulin are usually not specific to tumor cells and often cause a series of side effects such as cell division disorders in healthy tissues
Not only that, the resistance to chemotherapy drugs in clinical treatment has become a very serious problem, which greatly reduces the quality of life of some cancer patients

Method used

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  • Bacillus marinus and application thereof
  • Bacillus marinus and application thereof
  • Bacillus marinus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, the acquisition of marine bacillus:

[0037] In March 2016, followed the "Science" to collect sea mud samples in the seamount area of ​​the western Pacific Ocean (139°3802'E, 11°44162'N). The collected samples were serially diluted with sterile distilled water, and 10 -6 ,10 -5 ,10 -4 0.2 ml each of the diluted solution was spread on the solid medium, and cultured at 28 degrees for 48 hours. The colonies were transferred to fresh solid medium (5g tryptone, 1g yeast extract in 1L sterile seawater, and 15 agars were added) by the tetrad streak method, and transferred several times until the pure culture of the strain was obtained. This strain is stored in the General Microbiology Center of China Microbiological Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing (Institute of Microbiology, Chinese Academy of Sciences), and the preservation date is December 2017. , deposit number: CGMCC NO.14928. ...

Embodiment 2

[0042] Preparation of Exopolysaccharide from Bacillus sp.11

[0043] Pick a single colony and inoculate it into a test tube containing 5ml of liquid 2216E medium, and culture it with shaking at 28°C and 160rpm for 24h to obtain a seed fermentation broth. The seed fermentation broth of the Bacillus was inoculated into 500 ml of 2216e medium with 1% sucrose at a ratio of 0.1%, and cultured at 28° C. and 160 rpm for 48 hours with shaking. Centrifuge (8000rpm, 20min) to remove the bacteria, then add 3 times the volume of supernatant 95% ethanol solution to precipitate overnight at 4°C, then centrifuge (8000rpm, 20min, 4°C) to take the precipitate, and add water to dissolve all the precipitate. After dissolving, the protein was removed by Sevage reagent three times, and then the solution was put into a dialysis bag of 8000-14000Da for dialysis. The dialysate was filtered through a 0.22μm filter membrane, condensed with an anion column, and eluted with 20mM Tris and 2M NaCl. The e...

Embodiment 3

[0046] Effects of EPS11 administered in vitro on the proliferation activity of lung cancer A549 cells

[0047] experimental method

[0048] Take the A549 cells in the logarithmic growth phase, digest the cell solution in the logarithmic growth phase into a single cell suspension with trypsin, count the cells, and divide the cells according to the ratio of 6×10 3 The density of cells / well was seeded in 96-well cell culture plate, and placed in 5% CO 2 , 37°C cell culture incubator; the next day, add EPS11 with final concentrations of 0, 0.01, 0.02, 0.04, 0.10, 0.20, 0.50, 1.00 and 2.00mg / ml to the 96-well plate, and set the concentration of each group 3 duplicate wells were put into the incubator to continue culturing for 24h and 48h respectively. After being treated with EPS11, 30μl of MTT solution (concentration: 5mg / ml) was added to each well, placed in the incubator and then cultured for 4h, and then each well was Then add 100 μl of triple solution, put it into the incuba...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to bacillus marinus and application thereof. The bacillus marinus is a polysaccharide producing marine bacterium Bacillus sp. BS11; a strain is applied to the preparation of an anti-cancer drug, an anti-tumor metastasis drug, an anti-cancer health product or an anti-cancer recurrence health product; thebacillus marinus provided by the invention is separated from the seamount and can be used for preparing the anti-cancer drug or the anti-cancer health product or preparing the anti-tumor metastasis drug or an anti-tumor formation health product. The invention discloses application of an extracellular polysaccharide of a bacillus to the preparation of anti-cancer and anti-tumor metastasis drugs andhealth products, and the bacillus marinus can be used for inhibiting the expression, in a cell A549, of betaIII-tubulin, is used for inhibiting the formation of byssus on the surface of a cancer cell, inhibiting the adherence and the attachment of the cell A549 and inhibiting the metastasis of the cell A549, is finally used for causing the anoikis of the cell, and therefore, has certain prevention and treatment effects of malignant tumor.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a marine bacillus and its application. Background technique [0002] Lung cancer is the leading cause of cancer-related death in the world population, the effectiveness of current treatment is severely limited, and the mortality rate is extremely high. Among them, non-small cell lung cancer accounts for 80% of all types of lung cancer, and chemotherapy is still the standard method of treatment. Although these therapeutic strategies have greatly improved patients' symptoms and quality of life, the overall survival rate of patients remains low. Therefore, the need to find and develop novel and effective drugs to prevent and treat lung cancer is still very urgent. [0003] β-tubulin is an important cytoskeletal protein involved in a variety of important cellular processes, including mitosis, intracellular material transport and so on. In cancer cells, β-tubulin is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04A61K35/742A61P35/00A61P35/04A23L33/125C12R1/07
CPCA23V2002/00A61K2035/115A23L33/125C12P19/04C12N1/205C12R2001/07A23V2200/308A23V2200/30
Inventor 孙超岷曹若冰刘格
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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