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Methods to reduce chlorella adherence

A technology of chlorella and common chlorella, applied in the biological field, can solve problems such as harsh living environment, wall attachment, and difficulty in algal cell uptake of nutrients

Active Publication Date: 2020-09-22
杭州园泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the cultivation process, due to gravity, uneven aeration and the physiological characteristics of the algae, etc., the inner wall of the cultivation device or the objects in contact with the algae liquid often appear adherence
Slight adherence will not affect the growth of algal cells, but once large-scale adherence occurs, it will produce a vicious circle and form adverse effects, making it difficult for algal cells to absorb nutrients, poor living environment and insufficient light. Avoiding the occurrence of large-scale adherence in the cultivation of Chlorella is a technical problem that we need to solve
There are relatively few studies on the above problems in the prior art, and there are only a few reports at present. Among them, "CN105754861A" discloses a method for reducing the adhesion of chlorella, including adding an emulsifier to the culture medium , can reduce the adherence of chlorella and increase the biomass of chlorella; although the patent technology believes that adding emulsifiers will not have an adverse effect on the growth of chlorella, a small amount of emulsifiers will inevitably remain in the algae extract In order to improve the quality of the algae extract, we should try to avoid the residue of chemical substances such as emulsifiers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for reducing chlorella adherence, comprising the steps of:

[0026] The Chlorella vulgaris liquid in the logarithmic growth phase was inoculated into the reaction tank containing the culture solution according to the inoculation amount of 5%, and the inoculation density was 1×10 6 1 / ml, then add 100mg / L amino acid, cultivate for 96h, centrifuge, and collect the algae cells; the culture conditions are: light intensity 5000lux, culture at 22°C, light-to-dark ratio of 18:6, ventilation rate of 0.2vvm; The components of the culture medium are: glucose 3g / L, potassium nitrate 1.5g / L, sodium chloride 1g / L, borax 0.5g / L, potassium dihydrogen phosphate 0.5g / L, calcium carbonate 0.1g / L, heptahydrate Magnesium sulfate 50 mg / L, ferric ammonium citrate 20 mg / L, sodium naphthalene acetate 10 mg / L, gibberellin 10 mg / L; the amino acid is histidine.

Embodiment 2

[0028] A method for reducing chlorella adherence, comprising the steps of:

[0029] The Chlorella vulgaris liquid in the logarithmic growth phase was inoculated into the reaction tank containing the culture solution according to the inoculation amount of 5%, and the inoculation density was 2×10 6 1 / ml, then add 100mg / L amino acid, cultivate for 96h, centrifuge, and collect algae cells; the culture conditions are: light intensity 5000lux, culture at 22°C, light-to-dark ratio 14:10, ventilation rate 0.3vvm; The components of the culture medium are: glucose 3g / L, potassium nitrate 1.5g / L, sodium chloride 1g / L, borax 0.5g / L, potassium dihydrogen phosphate 0.5g / L, calcium carbonate 0.1g / L, heptahydrate Magnesium sulfate 50 mg / L, ferric ammonium citrate 20 mg / L, sodium naphthalene acetate 10 mg / L, gibberellin 10 mg / L; the amino acid is a mixture of histidine and arginine in a 1:1 mass ratio.

Embodiment 3

[0043] The influence of chlorella adherence and biomass in embodiment 1-2 and comparative example 1-4:

[0044] Observe the growth of algal cells in each group, and divide the phenomenon of algal cell adhesion into four grades. Among them, the first grade is very serious, and the algal cells are aggregated into blocks, tightly combined, and a large number of deaths; the second grade Grade 3: slightly adherent, algal cells aggregated loosely, and 0 or a small amount of death.

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Abstract

The invention belongs to the field of biotechnology, and discloses a method for reducing the adherence of chlorella. The method comprises the following steps: inoculating 3-10% of chlorella liquid ina logarithmic growth phase to a reaction cell containing a culture solution, then adding 50-200 mg / L of amino acid, culturing for 96-120 h, centrifuging, and collecting chlorella cells. The method canreduce the adherence of the chlorella and improve the biomass of the chlorella.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for reducing chlorella adherence. Background technique [0002] Chlorella (Chlorella) is a general unicellular green algae of the Chlorella phylum Chlorella. It is a spherical single-celled algae with a diameter of 3 to 8 microns. It is one of the earliest life on earth and appeared in more than 2 billion years. Formerly, it is a highly efficient photosynthetic plant that grows and reproduces with photosynthetic autotrophy, and has a wide distribution. With the help of sunlight, water and carbon dioxide, Chlorella has the vigorous reproductive ability of dividing into 4 cells every 20 hours, continuously transforms the solar energy into algae containing various nutrients, and releases a large amount of Oxygen; and its photosynthetic capacity is more than 10 times higher than other plants. Chlorella can not only be used as an excellent natural bait for aquatic e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 胡叶飞张菊萍董国强张宏杰金荣培
Owner 杭州园泰生物科技有限公司
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