Culture medium for producing spores, and optimizing process and application thereof

A culture medium and spore-producing technology, which is applied in the field of culture medium for producing highly active spores of marine Bacillus licheniformis and the medium for high-production active spores of marine Bacillus, which can solve the problems of long shelf life and obstacles

Active Publication Date: 2012-07-04
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, marine Bacillus licheniformis 9912 is not easy to produce spores under conventional culture conditions, which seriously hinders its development into an active spore preparation that is highly efficient, non-toxic, l

Method used

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  • Culture medium for producing spores, and optimizing process and application thereof
  • Culture medium for producing spores, and optimizing process and application thereof
  • Culture medium for producing spores, and optimizing process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Activation of strains

[0024] Marine Bacillus licheniformis 9912 (preservation number: CCTCC M2010343), marine Bacillus 3728 (preservation number: CCTCC M207052, Hu Jiangchun, Xu Hongli, Liu Li, etc., a marine Bacillus subtilis and its screening method, patent application number: 200710011179.0) , marine Bacillus subtilis 3512A (preservation number: CCTCC M20705, Hu Jiangchun, Li Wei, Liu Li, Xue Delin, Wang Nan, Pan Huaqi, Wang Shujin, preparation of marine Bacillus subtilis for the prevention and treatment of cucumber wilt, patent application number: 200810011342.8) were inoculated by marking in The modified starch agar medium was cultured at 28±1°C for 48 hours. After picking and observing under a microscope, a typical single colony was streaked and purified again, then inoculated on a slant, and cultured at 28±1°C for 48 hours for later use. Among them, the typical colony morphology of marine Bacillus licheniformis on the modified starch agar medium is irregular...

Embodiment 2

[0034] The optimization of the above-mentioned marine bacillus high-yield spore culture medium of embodiment 2

[0035] 1. Single factor multilevel experimental design

[0036] Use cheap industrial medium (1.0% cornmeal, 0.1% soybean meal, 0.3% wheat bran, 0.05% potassium dihydrogen phosphate, natural pH) as the base medium, by changing the state of the inoculated bacteria, the inoculation amount, and the culture temperature , the amount of dissolved oxygen, the initial pH of the fermentation broth, the addition of divalent metal ions, the culture time and other factors, the effects of various factors on the spore formation and the total number of spores of Bacillus licheniformis 9912 were investigated. It is screened that inoculation with spore state is the key factor limiting spore generation, the spore generation rate is less than 20% when inoculated with non-spore state, and the spore generation rate exceeds more than 80% after inoculation with spore state. It is prelimin...

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Abstract

The invention provides a culture medium for producing spores, and the optimizing process and application thereof, which are particularly applied to marine bacillus which hardly generates spores in a common liquid medium. The culture medium comprises the following components in percentage by weight: 0.5 to 3 percent of cornmeal, 0.1 to 1 percent of bran, 0.1 to 0.5 percent of soybean cake meal, 0.01 to 0.1 percent of potassium dihydrogen phosphate dodecahydrate, 0.05 to 0.3 percent of calcium carbonate anhydrous and the balance of water. The culture medium has the advantages of readily available raw materials, low cost and simple preparation, and can obviously improve the spore formation rate and total active spores of marine bacillus 9912 and marine bacillus subtilis 3728. By the culture medium and the application of the culture medium, essential conditions are provided for developing bacillus licheniformis 9912 active spore preparations and other related active spore preparations.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and more specifically relates to a culture medium for high-yield active spores of marine bacillus optimized by response surface method and its application, especially a kind of medium for producing high active spores of marine bacillus licheniformis which is not easy to produce spores culture medium and its application. Background technique [0002] Fungal diseases have brought huge losses to agriculture and forestry. At present, the control of crop diseases mainly depends on the use of broad-spectrum chemical pesticides. However, the extensive use of chemical pesticides has led to increasing resistance of pests and diseases, high pesticide residues and serious pollution in agricultural and livestock products, food and water sources have been seriously polluted, and human health and the ecological environment have been seriously threatened. Therefore, it is very necessary and urgent to grad...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/10
Inventor 潘华奇胡江春刘丽王楠李学红李春田杨永军牛力轩王书锦
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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