Bacillus marinus with multiple plant functions and application thereof
A marine bacillus, functional technology, applied in the application, plant growth regulator, plant growth regulator and other directions, can solve the problems of poor soil-borne disease control effect, difficult survival, colonization, limitation, etc., to achieve biological characteristics and The effect of stable pesticide activity, promotion and induction of plant salt resistance, and low production cost
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Embodiment 1
[0018] Example 1: Artificial cultivation and fermentation of BY-1188 strain
[0019] With the deposit number: CGMCC No. 6187 BY-1188 strain, after medium optimization test, its suitable culture fermentation broth formula is: peptone 2-5g, yeast powder 1-3g, NaCl, 25-30g, KCl0. 3-0.5g, MgCl 2 ·7H 2 O1.9-2.5g, FePO 4 0.01-0.02g, 1000ml water, pH 7.0-7.5, the above is the culture solution, and 16-19g agar is added as a solid medium.
[0020] Cultivate on the above-mentioned solid medium and test its physical and chemical characteristics by conventional methods. The colony and bacterial characteristics of this strain: the surface of the colony is rough and opaque, the early stage is grayish white, and then it turns into turbid red, the edges are notched, and the surface is pleated; Straight rod-shaped, Gram staining positive, 0.5-2.5μm×1.2-10μm, no capsule, peripheral flagellum, spores are not enlarged, arranged in pairs or chains;
[0021] Physiological and biochemical characteristics:...
Embodiment 2
[0023] Example 2: The antibacterial activity of BY-1188 strain against a variety of plant pathogenic fungi;
[0024] The antibacterial activity of the fermentation broth adopts 3 kinds of combined antibacterial test methods (confrontation culture method, 96-well plate method, and antibacterial rate statistical method) to test the antibacterial activity of strains. This combined test method can effectively avoid human error and The strain is false positive, and the obtained strain is more practical.
[0025] Among them, the confrontation culture method and the statistical method of inhibition rate are conventional methods, and 9 kinds of plant pathogenic fungi that are common and harmful to agriculture: Pyricularia oryzae, Alternaria salani, Fusarium oxysporrum, Botrytis cinerea Botrytis cinerea, Verticillium dahliae, Cladosporium fulvum, Phytophthora capsici, Rhizoctonia solani Fusarium graminearum were tested for bacteriostasis;
[0026] The 96-well plate method is the applicant's ...
Embodiment 3
[0039] Example 3: Colonization of BY-1188 strain in living plants
[0040] The tested methods are the conventional test methods for plant colonization: BY-1188 is marked with rifampicin and then cultured and fermented in the method of Example 1. The tested plant seedlings are immersed in the fermentation broth and planted into common farming soil and seashore saline soil (NaCl The content of 0.5%) in pots and pots, each tissue of the seedlings were ground on 1, 3, 5, 7, 10, and 15 days, and samples were cultured and observed on a plate medium supplemented with Rifu, and converted into bacterial colonies per gram of sample The number (cfu / g), the result is shown in Table 2. The BY-1188 strain can be planted normally in ordinary farming soil or seaside saline soil. Cucumbers, tomatoes, peppers, cabbage, melon, and eggplant can be planted normally, and root-dipping planting The highest colonization number of roots, stems and leaves after 5 days can reach 5.98×10 3 , 6.24×10 3 , 3.78...
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