Bacillus marinus with multiple plant functions and application thereof

A marine bacillus, functional technology, applied in the application, plant growth regulator, plant growth regulator and other directions, can solve the problems of poor soil-borne disease control effect, difficult survival, colonization, limitation, etc., to achieve biological characteristics and The effect of stable pesticide activity, promotion and induction of plant salt resistance, and low production cost

Inactive Publication Date: 2015-09-23
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the development of microbial pesticides at home and abroad, because the biocontrol bacteria are derived from land, it is difficult to survive a

Method used

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  • Bacillus marinus with multiple plant functions and application thereof
  • Bacillus marinus with multiple plant functions and application thereof
  • Bacillus marinus with multiple plant functions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Artificial cultivation and fermentation of BY-1188 strain

[0019] With the deposit number: CGMCC No. 6187 BY-1188 strain, after medium optimization test, its suitable culture fermentation broth formula is: peptone 2-5g, yeast powder 1-3g, NaCl, 25-30g, KCl0. 3-0.5g, MgCl 2 ·7H 2 O1.9-2.5g, FePO 4 0.01-0.02g, 1000ml water, pH 7.0-7.5, the above is the culture solution, and 16-19g agar is added as a solid medium.

[0020] Cultivate on the above-mentioned solid medium and test its physical and chemical characteristics by conventional methods. The colony and bacterial characteristics of this strain: the surface of the colony is rough and opaque, the early stage is grayish white, and then it turns into turbid red, the edges are notched, and the surface is pleated; Straight rod-shaped, Gram staining positive, 0.5-2.5μm×1.2-10μm, no capsule, peripheral flagellum, spores are not enlarged, arranged in pairs or chains;

[0021] Physiological and biochemical characteristics:...

Embodiment 2

[0023] Example 2: The antibacterial activity of BY-1188 strain against a variety of plant pathogenic fungi;

[0024] The antibacterial activity of the fermentation broth adopts 3 kinds of combined antibacterial test methods (confrontation culture method, 96-well plate method, and antibacterial rate statistical method) to test the antibacterial activity of strains. This combined test method can effectively avoid human error and The strain is false positive, and the obtained strain is more practical.

[0025] Among them, the confrontation culture method and the statistical method of inhibition rate are conventional methods, and 9 kinds of plant pathogenic fungi that are common and harmful to agriculture: Pyricularia oryzae, Alternaria salani, Fusarium oxysporrum, Botrytis cinerea Botrytis cinerea, Verticillium dahliae, Cladosporium fulvum, Phytophthora capsici, Rhizoctonia solani Fusarium graminearum were tested for bacteriostasis;

[0026] The 96-well plate method is the applicant's ...

Embodiment 3

[0039] Example 3: Colonization of BY-1188 strain in living plants

[0040] The tested methods are the conventional test methods for plant colonization: BY-1188 is marked with rifampicin and then cultured and fermented in the method of Example 1. The tested plant seedlings are immersed in the fermentation broth and planted into common farming soil and seashore saline soil (NaCl The content of 0.5%) in pots and pots, each tissue of the seedlings were ground on 1, 3, 5, 7, 10, and 15 days, and samples were cultured and observed on a plate medium supplemented with Rifu, and converted into bacterial colonies per gram of sample The number (cfu / g), the result is shown in Table 2. The BY-1188 strain can be planted normally in ordinary farming soil or seaside saline soil. Cucumbers, tomatoes, peppers, cabbage, melon, and eggplant can be planted normally, and root-dipping planting The highest colonization number of roots, stems and leaves after 5 days can reach 5.98×10 3 , 6.24×10 3 , 3.78...

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Abstract

The present invention discloses a bacillus marinus with multiple plant functions, bacillus marinus BY-1188 strain is obtained from marine living condition, artificial mutation breeding and optimization of culture conditions are performed, the accession number of the BY-1188 strain is CGMCC No. 6187, and through culture optimization tests of the strain, the optimum fermentation broth formula is as follows: 2-5g of peptone, 1-3g of yeast powder, 25-30g of NaCl, 0.3-0.5g of KCl, 1.9-2.5g of MgCl2 . 7H2O, 0.01-0.02g of FePO4 and 1000ml of water, pH is 7.0-7.5, the culture medium is added with 16-19g of agar to be used as a solid medium. The bacillus marinus has the advantages of biological characteristics and stable agrochemical activity, and has no environmental pollution.

Description

Technical field [0001] The present invention belongs to the field of marine microorganisms, specifically, it is a bacillus obtained from marine habitats. The species name is Bacillus marinus. Through artificial mutagenesis, breeding, culture and fermentation, live bacterial preparations can prevent diseases and promote cultivation of plants. Growth, induction of cold and salt resistance. This strain is deposited in the General Microbiology Center (CGMCC) of the China Microbial Culture Collection Management Committee, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, preservation date June 5, 2012, preservation Number CGMCC No.6187, classified and named Bacillus marinus. Background technique [0002] The safety of agricultural products is the source of all food safety. The development and use of high-efficiency and low-toxic biological pesticides are the basic measures to ensure the safety of agricu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
Inventor 田黎
Owner QINGDAO UNIV OF SCI & TECH
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