Hairtail activity extract for articulation restoration
A technology of bone joints and extracts, applied in bone diseases, drug combination, fermentation, etc., can solve the problems of low enzymatic hydrolysis efficiency, achieve high absorption and utilization rate, delay osteoporosis, and increase bone density
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Embodiment 1
[0017] The hairtail active extract for bone and joint repair is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The hairtail fish meat homogenate was hydrolyzed with Porphyra zebra protease, and the hydrolysis was assisted by ultrasonic waves. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porphyra zebra protease to enzymatically hydrolyze hairtail protein at the same time, and a large number of active peptides can be obtained .
[0018] The freeze-drying steps are: pre-freezing: -40°C for 5 hours; freeze-drying: -40°C, 4Pa freeze-drying for 6 hours, -7°C, 16Pa freeze-drying for 2.6 hours; sublimation drying: 13°C, 57Pa sublimation drying for 3 hours; analytical drying : 10 ℃, 4Pa analysis and drying for 3 hours, to obtain the hairtail active extract for bone and...
Embodiment 2
[0026] The hairtail active extract for bone and joint repair is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The hairtail fish meat homogenate was hydrolyzed with Porphyra zebra protease, and the hydrolysis was assisted by ultrasonic waves. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porphyra zebra protease to enzymatically hydrolyze hairtail protein at the same time, and a large number of active peptides can be obtained .
[0027] The freeze-drying steps are: pre-freezing: -40°C for 5 hours; freeze-drying: -40°C, 3Pa freeze-drying for 5h, -5°C, 12Pa freeze-drying for 2h; sublimation drying: 13°C, 52Pa sublimation drying for 3.4h; analytical drying : 10 ℃, 4Pa analysis and drying for 3 hours, to obtain the hairtail active extract for bone and joint repair. ...
Embodiment 3
[0035] The hairtail active extract for bone and joint repair is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The specific preparation steps are:
[0036] 1) Compound enzyme enzymatic hydrolysis: use alkaline protease produced by Bacillus marinum and Porphyra zebra protease to enzymatically hydrolyze hairtail fish meat homogenate, and use ultrasonic-assisted enzymatic hydrolysis. The addition amount of alkaline protease produced by Bacillus marine bacillus and Porphyra zebra protease was 1.4wt%, the enzymolysis temperature was 30°C, the pH was 8.0, the enzymolysis time was 44min, and the ultrasonic frequency was 25kHZ. After the enzymatic hydrolysis, the enzyme was inactivated in a boiling water bath for 3 min. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porp...
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