Hairtail activity extract for articulation restoration

A technology of bone joints and extracts, applied in bone diseases, drug combination, fermentation, etc., can solve the problems of low enzymatic hydrolysis efficiency, achieve high absorption and utilization rate, delay osteoporosis, and increase bone density

Inactive Publication Date: 2017-10-13
兰溪市沉默生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The hairtail active extract for bone and joint repair is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The hairtail fish meat homogenate was hydrolyzed with Porphyra zebra protease, and the hydrolysis was assisted by ultrasonic waves. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porphyra zebra protease to enzymatically hydrolyze hairtail protein at the same time, and a large number of active peptides can be obtained .

[0018] The freeze-drying steps are: pre-freezing: -40°C for 5 hours; freeze-drying: -40°C, 4Pa freeze-drying for 6 hours, -7°C, 16Pa freeze-drying for 2.6 hours; sublimation drying: 13°C, 57Pa sublimation drying for 3 hours; analytical drying : 10 ℃, 4Pa analysis and drying for 3 hours, to obtain the hairtail active extract for bone and...

Embodiment 2

[0026] The hairtail active extract for bone and joint repair is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The hairtail fish meat homogenate was hydrolyzed with Porphyra zebra protease, and the hydrolysis was assisted by ultrasonic waves. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porphyra zebra protease to enzymatically hydrolyze hairtail protein at the same time, and a large number of active peptides can be obtained .

[0027] The freeze-drying steps are: pre-freezing: -40°C for 5 hours; freeze-drying: -40°C, 3Pa freeze-drying for 5h, -5°C, 12Pa freeze-drying for 2h; sublimation drying: 13°C, 52Pa sublimation drying for 3.4h; analytical drying : 10 ℃, 4Pa analysis and drying for 3 hours, to obtain the hairtail active extract for bone and joint repair. ...

Embodiment 3

[0035] The hairtail active extract for bone and joint repair is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The specific preparation steps are:

[0036] 1) Compound enzyme enzymatic hydrolysis: use alkaline protease produced by Bacillus marinum and Porphyra zebra protease to enzymatically hydrolyze hairtail fish meat homogenate, and use ultrasonic-assisted enzymatic hydrolysis. The addition amount of alkaline protease produced by Bacillus marine bacillus and Porphyra zebra protease was 1.4wt%, the enzymolysis temperature was 30°C, the pH was 8.0, the enzymolysis time was 44min, and the ultrasonic frequency was 25kHZ. After the enzymatic hydrolysis, the enzyme was inactivated in a boiling water bath for 3 min. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porp...

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PUM

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Abstract

The invention discloses a hairtail activity extract for articulation restoration. The hairtail activity extract is prepared by compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC (High Performance Liquid Chromatography) elution and freeze drying, wherein the compound enzyme enzymolysis comprises the steps of performing enzymolysis on hairtail meat homogenate by adopting marine bacillus produced alkaline protease and porphyra yezoensis protease, and performing ultrasonic for assisting in the enzymolysis. The hairtail activity extract has the benefits that the composition and a sequence of amino acid of marine organism protein are very different from those of terrestrial organism protein; when the marine bacillus produced alkaline protease and the porphyra yezoensis protease simultaneously perform the enzymolysis on hairtail protein, abundant activated peptide fragments can be obtained; and the obtained hairtail activity extract can promote generation of cartilage cells, can greatly hasten and supplement articulation synovia, can effectively promote generation of collagen stroma, stimulates regeneration of connective tissue cells, and further accelerates reconstruction and recovery of a soft tissue.

Description

technical field [0001] The invention relates to the technical field of health care products, in particular to an active hairtail extract for bone and joint repair. Background technique [0002] Peptides have only been paid attention to since the beginning of the 20th century. Studies have found that they are different from proteins and amino acids, and have the effect of drugs. It was not until the 1990s that there was considerable development. Under the guidance of constantly updated technologies, the scope of application of polypeptides became wider and wider, and their functions became more and more important. Only then did they enter our lives and occupy an important role in our lives. The share of peptide drugs is increasing, high efficiency, low toxicity and strong specificity have unique advantages different from other drugs, which are constantly affecting our lives. Moreover, the potential of peptides is huge, which is another major breakthrough and development dire...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16A61P19/02
CPCC12P21/06C07K1/16C07K1/34C07K1/36
Inventor 周建波
Owner 兰溪市沉默生物科技有限公司
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