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Method for preparing flocculating agent by using Bacillus marinus

A marine bacillus and flocculant technology, which is applied in the field of preparing flocculants by using marine bacillus, can solve the problems of low polysaccharide flocculation activity and low polysaccharide yield, and achieve the effects of saving ethanol, simplifying separation steps, and reducing production costs

Inactive Publication Date: 2019-02-15
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of microbial flocculant-producing bacteria have been screened from soil, activated sludge, domestic sewage, and various industrial wastewater environments. Among them, Aspergillus sojae AJ7002 and Takagi which produce microbial flocculant AJ7002 discovered by Nakamura et al. Paecilomyces producing PF101 and Rhodococcus erythrococcus producing NOC-1 such as Kurane, etc., these microbial flocculants have the advantages of environmental friendliness and no secondary pollution compared with traditional polymer chemical flocculants, but these Microorganisms have the problems of low polysaccharide yield and low polysaccharide flocculation activity

Method used

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  • Method for preparing flocculating agent by using Bacillus marinus
  • Method for preparing flocculating agent by using Bacillus marinus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The liquid medium includes the following components: glucose 13g / L, sucrose 8g / L, peptone 1.5g / L, sodium acetate 0.8g / L, yeast extract 0.7g / L, potassium dihydrogen phosphate 0.8g / L, and the balance is Aged sea water.

[0030] Solid medium: a slant medium prepared by adding 1.5% agar by weight of the liquid medium to the liquid medium and solidifying it.

[0031] The method for preparing flocculant by using marine bacillus comprises the following steps:

[0032] 1) Inoculate the marine Bacillus strain into the solid medium, culture at 27°C for 55 hours, and then add sterile water twice the volume of the solid medium to make a seed solution;

[0033] 2) inoculating the strain liquid into a liquid medium according to the inoculum size of 15% and carrying out fermentation and cultivation at 24° C. for 4 days to obtain a fermentation liquid;

[0034] 3) The flocculation carrier is added to the fermentation broth at a mass volume ratio of 0.45 g / ml, the pH is adjusted to 7....

Embodiment 2

[0039] The liquid medium includes the following components: glucose 11g / L, sucrose 6g / L, peptone 1.5g / L, sodium acetate 0.6g / L, yeast extract 0.7g / L, potassium dihydrogen phosphate 0.6g / L, and the balance is Aged sea water.

[0040] Solid medium: a slant medium prepared by adding 1.5% agar by weight of the liquid medium to the liquid medium and solidifying it.

[0041]The method for preparing flocculant by using marine bacillus comprises the following steps:

[0042] 1) Inoculate the marine Bacillus strain into a solid medium, culture it at 23°C for 52 hours, and then add sterile water 1.8 times the volume of the solid medium to make a seed solution;

[0043] 2) inoculating the strain liquid into a liquid medium according to the inoculum size of 13% and carrying out fermentation and cultivation at 24° C. for 4 days to obtain a fermentation liquid;

[0044] 3) The flocculation carrier is added into the fermentation broth at a mass volume ratio of 0.3 g / ml, the pH is adjusted ...

Embodiment 3

[0049] The liquid medium includes the following components: glucose 14g / L, sucrose 8g / L, peptone 1.5g / L, sodium acetate 0.8g / L, yeast extract 0.9g / L, potassium dihydrogen phosphate 0.8g / L, and the balance is Aged sea water.

[0050] Solid medium: a slant medium prepared by adding 1.5% agar by weight of the liquid medium to the liquid medium and solidifying it.

[0051] The method for preparing flocculant by using marine bacillus comprises the following steps:

[0052] 1) Inoculate the marine Bacillus strain into a solid medium, culture it at 28°C for 58 hours, and then add sterile water 2.5 times the volume of the solid medium to make a seed solution;

[0053] 2) inoculating the strain liquid into a liquid medium according to the inoculum size of 16% and carrying out fermentation and cultivation at 24° C. for 5 days to obtain a fermentation liquid;

[0054] 3) The flocculation carrier is added into the fermentation broth at a mass volume ratio of 0.4 g / ml, the pH is adjusted...

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Abstract

The invention relates to the technical field of flocculating agent preparation, and solves problems of low polysaccharide yield and low polysaccharide flocculation activity in an existing microorganism. The invention discloses a method for preparing a flocculating agent by using Bacillus marinus. The method for preparing the flocculating agent by using the Bacillus marinus comprises the followingsteps: firstly, inoculating a Bacillus marinus strain into a solid culture medium and adding sterile water to the solid culture medium to prepare a strain liquid; secondly, inoculating the strain liquid into a liquid culture medium according to an inoculating quantity to ferment and culture the strain liquid so as to obtain a fermentation liquor; thirdly, adding a flocculation carrier to the fermentation liquor to continue to ferment the fermentation liquor so as to obtain a raw material liquor; and centrifugally separating and drying the raw material liquor to obtain an active polysaccharideflocculating agent. The method for preparing the flocculating agent by using the Bacillus marinus, disclosed by thei nvention, has the advantages that the yield of the active polysaccharide produced by using the Bacillus marinus strains is higher, and the flocculation activity of the active polysaccharide produced by using the Bacillus marinus strains is also higher.

Description

technical field [0001] The invention relates to the technical field of flocculants, in particular to a method for preparing flocculants by using marine bacillus. Background technique [0002] Flocculants are one of the commonly used agents in water treatment technology. In recent years, the most widely used traditional high-molecular polymer flocculants have been found to have potential problems that threaten human health and destroy the ecological environment. Research on new environmentally friendly and pollution-free flocculants has become a hot issue in water treatment research. Most of them are microbial flocculants. Bioflocculants are a class of polymers with biodegradable functions, including polysaccharides, lipids and proteins secreted by microorganisms with such special functions. Because of its safety and non-pollution to the environment, it is valued by many scientific researchers at home and abroad. At present, a variety of microbial flocculant-producing bact...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/08C12R1/07
CPCC08B37/003C12P19/04
Inventor 穆军
Owner ZHEJIANG OCEAN UNIV
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