Bacillus marinus bacterium agent as well as preparation method and application thereof

A technology of marine bacillus and bacteria agent, which is applied in the field of agricultural microorganisms, can solve the problems of single planting species, unscientific medication, and decreased soil fertility, and achieve the effects of improving soil fertility, simple and efficient preparation methods, and promoting plant growth

Inactive Publication Date: 2017-08-25
青岛康菲斯顿生物科技有限公司
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Problems solved by technology

[0002] In recent years, due to the continuous expansion of continuous cropping area, unscientific drug use, single crop species in production, high-density continuous cropping cul...

Method used

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Experimental program
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Embodiment Construction

[0019] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0020] Part 1: Getting Marine Bacillus

[0021] 1. Screen the isolated strains

[0022] Dilute the water sample collected from Qingdao Ocean, Shandong Province by 10 -5 , spread evenly on LB medium for isolation and culture, and select single colonies.

[0023] 2. Identification of strains

[0024] The 16s sequence was used to carry out molecular identification on the selected single colony, and the identification results showed that the single colony was marine Bacillus.

[0025] 3. Strain preservation

[0026] We deposited the marine bacillus obtained by screening and isolation in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing) on ​​March 31, 2017, and the preservation number is CGMCCNo.13962.

[0027] Part II: Preparation of marine bacillus inoculum

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Abstract

The invention discloses a bacillus marinus bacterium agent as well as a preparation method and application thereof. The bacterium agent is prepared from bacillus marinus bacterium powder and humic acid, wherein the bacillus marinus bacterium powder is obtained by fermenting bacillus marinus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 13962 on a fermentation culture medium. The bacillus marinus bacterium agent has the beneficial effects that (1) the soil fertility can be improved and the growth of plants is promoted; a test verifies that the bacillus marinus bacterium agent disclosed by the invention can be used for remarkably promoting the growth of the plants and is adaptive to various crops; (2) the preparation method is simple and efficient and is suitable for industrial production.

Description

technical field [0001] The invention relates to a microbial bacterial agent and its preparation method and application, in particular to a marine bacillus bacterial agent and its preparation method and application, belonging to the technical field of agricultural microorganisms. Background technique [0002] In recent years, due to the continuous expansion of continuous cropping area, unscientific drug use, single crop species in production, high-density continuous cropping cultivation and lack of effective crop rotation and other cultivation measures, the soil fertility has declined, resulting in reduced crop production and quality. affected crop production. Contents of the invention [0003] The object of the present invention is to provide a marine bacillus bacterial agent capable of improving soil fertility and promoting plant growth and a preparation method thereof. [0004] In order to achieve the above object, the present invention adopts the following technical so...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05G3/04C12N1/20C05G3/80
CPCC05F11/02C05F11/08C05G3/80C12N1/20
Inventor 谢克虎
Owner 青岛康菲斯顿生物科技有限公司
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