Bacillus marinus S-1 and anti-tumor active polypeptide produced thereby

A marine bacillus, anti-tumor activity technology, applied in anti-tumor drugs, peptides, bacteria and other directions, can solve the problem that anti-tumor drugs cannot be compared with anti-tumor drugs

Active Publication Date: 2014-02-05
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, the number of marine microbial anticancer drugs is still not comparable to that of terrestrial sources

Method used

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  • Bacillus marinus S-1 and anti-tumor active polypeptide produced thereby
  • Bacillus marinus S-1 and anti-tumor active polypeptide produced thereby
  • Bacillus marinus S-1 and anti-tumor active polypeptide produced thereby

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] Embodiment 1--identification of bacterial strain S-1

[0134] 1. Screening and isolation of bacterial strains

[0135] 1) Cultivation of strains

[0136] A single bacterial strain was cultured, screened, and isolated from sea mud in the South China Sea, and stored in glycerin in a -80°C refrigerator. Randomly draw out 100 μL of glycerol-preserved bacterial strains, inoculate them into 30 ml of seed (activated) medium, and cultivate them at a constant temperature in a shaker at 30°C and 200 r / min for 24 hours. The seed medium: 10g / L peptone, 3g / L Glucose, 5g / L NaCl, pH=7.0, sterilized at 121°C for 30min.

[0137] 2) Fermentation of bacteria

[0138] The seed fermentation liquid was inoculated in 300 mL of fermentation medium according to the inoculum size of 4% (v / v), and the culture was continued for 36 h under the same conditions to obtain the strain fermentation liquid. The fermentation medium: peptone 10g / L, yeast extract 5g / L, 3g / L glucose, sodium chloride 5g / L,...

Embodiment 2

[0197] Embodiment 2---Fermentation culture of novel marine bacillus S-1 strain and its metabolite anti-tumor active polypeptide SBP:

[0198] 1. Seed cultivation

[0199] Inoculate 100 μL of glycerin-preserved bacterial strain (the bacterial strain cultivated, screened and isolated from the sea mud in the South China Sea—new marine bacillus S-1 or strain S-1 for short) from the -80°C refrigerator, and inoculate in 30ml of seed (activation) medium was cultured in a shaker at 30°C and 200r / min for 24h at a constant temperature. The seed medium: 10g / L peptone, 3g / L glucose, 5g / L NaCl, pH=7.0, sterilized at 121°C for 30min.

[0200] 2. Fermentation culture

[0201] Inoculate 4 (V / V)% of 20-h seed-age seeds, and cultivate them in a rotary shaker for 36 h at 30°C and 200 r / min. The fermentation medium: peptone 8-12g / L, yeast extract 3-6g / L, 3g / L glucose, sodium chloride 3-7g / L, 1mol / L NaOH to adjust the pH value to 6.5-7.5, 121°C Sterilize for 30min. 3. Anti-tumor polypeptide 3. ...

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Abstract

The invention relates to a bacillus marinus S-1 and an anti-tumor active polypeptide produced thereby. The bacillus marinus S-1 is cultivated, separated and screened from sea mud in the south china sea, so as to obtain a marine microbial bacteria strain of marine bacillus genus (Bacillus sp.) for producing the anti-tumor active polypeptide. The strain S-1 is aerobic bacteria, has good salt tolerance, and grows well in 7 w/v% of NaCl culture solution, wherein the growth pH range is 5.0-9.0; the growth temperature range is wide; the bacillus marinus S-1 can well grow in the environment of 15-37 DEG C; a gene sequence of 16S rDNA of the strain S-1 is shown in a figure. The polypeptide produced by the bacillus marinus S-1 has anti-tumor activity, and is expected to be an anti-tumor drug for treating a liver cancer.

Description

technical field [0001] The invention relates to the field of marine microorganisms and medicines, in particular to a marine bacillus S-1 (Bacillus S-1) and its anti-tumor active polypeptide. Background technique [0002] In today's society, malignant tumors seriously threaten human life and health, and are the number one killer of human society. Clinically, the treatment of malignant tumors mostly adopts comprehensive therapy, which combines surgical resection, radiotherapy, chemotherapy and immunotherapy. Although the cancer-related drugs currently on the market have certain curative effects on most cancers, they still have problems such as poor specificity, insignificant curative effect, and obvious toxic and side effects. Therefore, finding highly efficient, low-toxic, and highly specific antitumor drugs is still a major topic and long-term task in the field of biomedical research. [0003] The microbial world contains a lot of natural product resources, and marine micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C07K7/64A61P35/00C12R1/07
Inventor 孙谧郑兰红郝建华刘均忠衣尧
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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