Preparation method of hairtail active extract for repairing bone joints

A technology for bone joints and extracts, applied in the field of preparation of hairtail active extracts, can solve problems such as low enzymatic hydrolysis efficiency, and achieve the effects of stimulating connective tissue cell regeneration, small molecular weight, and inhibiting activity

Inactive Publication Date: 2017-11-03
兰溪市捷喜食品加工技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. The above method uses protease extracted from land to enzymatically hydrolyze marine organisms, and the enzymatic hydrolysis efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of the hairtail active extract used for bone and joint repair includes compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The hairtail fish meat homogenate was hydrolyzed with Porphyra zebra protease, and the hydrolysis was assisted by ultrasonic waves. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porphyra zebra protease to enzymatically hydrolyze hairtail protein at the same time, and a large number of active peptides can be obtained .

[0018] The freeze-drying steps are: pre-freezing: -40°C for 5 hours; freeze-drying: -40°C, 4Pa freeze-drying for 6 hours, -7°C, 16Pa freeze-drying for 2.6 hours; sublimation drying: 13°C, 57Pa sublimation drying for 3 hours; analytical drying : 10 ℃, 4Pa analysis and drying for 3 hours, to obtain the hairtail ac...

Embodiment 2

[0026] The preparation method of the hairtail active extract used for bone and joint repair includes compound enzyme enzymolysis, multi-stage membrane filtration, nanofiltration, HPLC elution and freeze-drying. The hairtail fish meat homogenate was hydrolyzed with Porphyra zebra protease, and the hydrolysis was assisted by ultrasonic waves. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porphyra zebra protease to enzymatically hydrolyze hairtail protein at the same time, and a large number of active peptides can be obtained .

[0027] The freeze-drying steps are: pre-freezing: -40°C for 5 hours; freeze-drying: -40°C, 3Pa freeze-drying for 5h, -5°C, 12Pa freeze-drying for 2h; sublimation drying: 13°C, 52Pa sublimation drying for 3.4h; analytical drying : 10 ℃, 4Pa analysis and drying for 3 hours, to obtain the hairtail active extract fo...

Embodiment 3

[0035] 1) Compound enzyme enzymatic hydrolysis: use alkaline protease produced by Bacillus marinum and Porphyra zebra protease to enzymatically hydrolyze hairtail fish meat homogenate, and use ultrasonic-assisted enzymatic hydrolysis. The addition amount of alkaline protease produced by Bacillus marine bacillus and Porphyra zebra protease was 1.4wt%, the enzymolysis temperature was 30°C, the pH was 8.0, the enzymolysis time was 44min, and the ultrasonic frequency was 25kHZ. After the enzymatic hydrolysis, the enzyme was inactivated in a boiling water bath for 3 min. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. Marine bacillus produces alkaline protease and Porphyra zebra protease to enzymatically hydrolyze hairtail protein at the same time, and a large number of active peptides can be obtained ;

[0036] 2) Multi-stage membrane filtration: first filter the filtrate with a 0.3µm m...

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Abstract

The invention discloses a preparation method of hairtail active extract for repairing bone joints; the preparation method comprises the steps of enzyme complex hydrolysis, multistage membrane filtration, nanofiltration, HPLC (high-performance liquid chromatography) eluting and lyophilizing; the step of enzyme complex hydrolysis includes enzymatically hydrolyzing hairtail flesh homogenate via Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase, and performing ultrasonic-assisted enzymatic hydrolysis. The preparation method has the advantages that marine organism proteins are greatly differed from terrestrial life proteins in both amino acid composition and amino acid sequence and hairtail proteins can be enzymatically hydrolyzed via the Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase to obtain mass active peptides; the obtained hairtail active extract can promote the generation of cartilage cells, joint synovial fluid can be massively induced and supplemented, the generation of collagen matrix can be effectively promoted, regeneration of connective tissue cells is stimulated, and the reconstruction and recovery of soft tissues are accelerated accordingly.

Description

technical field [0001] The invention relates to the technical field of preparation of health care products, in particular to a method for preparing an active hairtail extract for bone and joint repair. Background technique [0002] Peptides have only been paid attention to since the beginning of the 20th century. Studies have found that they are different from proteins and amino acids, and have the effect of drugs. It was not until the 1990s that there was considerable development. Under the guidance of constantly updated technologies, the scope of application of polypeptides became wider and wider, and their functions became more and more important. Only then did they enter our lives and occupy an important role in our lives. The share of peptide drugs is increasing, high efficiency, low toxicity and strong specificity have unique advantages different from other drugs, which are constantly affecting our lives. Moreover, the potential of peptides is huge, which is another m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
CPCC12P21/06C07K1/16C07K1/34C07K1/36
Inventor 张庆吉
Owner 兰溪市捷喜食品加工技术有限公司
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