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A kind of sulfadoxine residue reference substance with turbot muscle as matrix and preparation method thereof

A technology for sulfadoxine and turbot, which is applied in the field of sulfadoxine residue benchmarks and their preparation, can solve problems such as difficulty in extracting extraction solvents, and achieve the effect of scientific and accurate results.

Active Publication Date: 2015-10-28
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual fish muscle samples, sulfadoxine coexists in a bound form and a free form, and the extraction solvent is not easy to extract.

Method used

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  • A kind of sulfadoxine residue reference substance with turbot muscle as matrix and preparation method thereof
  • A kind of sulfadoxine residue reference substance with turbot muscle as matrix and preparation method thereof
  • A kind of sulfadoxine residue reference substance with turbot muscle as matrix and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of preparation method of the sulfadoxine residue reference material that takes turbot muscle as matrix, concrete method is as follows: the turbot that body weight is 500-600g / tail is cultured in seawater culture system, and culture system condition is: temperature is 18-21°C, salinity 31.7‰, dissolved oxygen value 4.7mg / L, pH value 8.1, circulating water, continuous aeration;

[0023] Take 50g of turbot special compound feed, dissolve it into a thin paste with 500ml of normal saline, add 100g of sulfadoxine and keep stirring to make the drug evenly distributed;

[0024] Extract the bait prepared above with a syringe (the amount of sulfadoxine in the extracted bait is 200mg / kg·bw), slowly extend the syringe into the mouth of the turbot, push the syringe, and push the bait into it; After that, stay for 2 to 3 seconds to prevent it from spitting out. If vomiting occurs, discard it and re-feed it.

[0025] Collect 10 back muscles of turbot on the 8th day after dosi...

Embodiment 2

[0028] The method in Example 1 was used to feed the turbot, and the residual concentration of sulfadoxine in turbot muscle collected from the first day to the 16th day after the bait was fed, as shown in the data table 1.

[0029] Table 1 The residual concentration of sulfadoxine in turbot muscle at different times

[0030] time (d)

Embodiment 3

[0032] Prepared Reference Material Homogeneity Test

[0033] Use the method in Example 1 to feed turbot, collect 10 turbot back muscles on the 8th day after feeding the medicine bait, peel off the skin, collect 100g of muscle for each fish, get 1000g altogether, and cut the muscle into 0.5cm×0.5 The meat pieces with the size of cm×0.5cm are homogenized after mixing, and divided into four equal parts by the method of quartering. For each sample, follow the Ministry of Agriculture Announcement No. 958-12-2007 "Measurement Solution for Sulfonamide Drug Residues in Aquatic Products Phase chromatography method" detection, the results are shown in Table 2.

[0034] Table 2 Reference substance homogeneity test results

[0035]

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Abstract

The invention relates to a sulfadoxine residual primary standard substance utilizing a turbot muscle as a matrix and a preparation method thereof. The preparation method comprises the specific steps of culturing the turbot the weight of which is 500-600g in a mariculture system, carrying out medicine preparation, filling and sampling the turbot. According to the sulfadoxine residual primary standard substance and the preparation method thereof provided by the invention, a sulfadoxine medicine in a turbot body can be really subjected to a digestion process, an absorbing process, a delivering process, an accumulating process, a metabolizing process and an eliminating process and other processes to be left in the muscle to prepare the standard substance, the sulfadoxine residual primary standard substance does not utilize the fish muscle as a base, and a medicine is added to prepare the standard substance, the property of the standard substance is close to the practical property of a detection sample, operations such as the evaluation of detection method, the inspection of the difference among laboratories, the verifying of the result accuracy of operators, which are performed by the substance, are scientific and exact.

Description

technical field [0001] The invention relates to the technical field of drug residue detection, in particular to a sulfadoxine residue reference substance with turbot muscle as a substrate and a preparation method thereof. Background technique [0002] The illegal use of sulfadoxine in the breeding process is serious, and the problem of residues in farmed fish is becoming more and more prominent. Sulfadoxine residues are one of the unsafe factors for eating farmed fish in my country. [0003] In order to detect sulfadoxine drug residues, there are many detection methods now, such as "Determination of 17 kinds of sulfonamides and 15 kinds of quinolones in aquatic products in Announcement No. 1077 of the Ministry of Agriculture-1-2008 Liquid chromatography-tandem mass spectrometry "," Ministry of Agriculture Announcement No. 958-12-2007 Liquid Chromatographic Method for the Determination of Sulfonamide Drug Residues in Aquatic Products", etc. [0004] In order to assess the ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06
Inventor 李兆新郭萌萌王英姿吴海燕翟毓秀
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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