Composite microorganism bacterial agent promoting tobacco leaf growth, applications and tobacco leaf cultivation method
A technology of compound microbial bacterial agent and cultivation method, which is applied in the field of compound microbial bacterial agent and its preparation, can solve the problems of poor systematic quality of tobacco leaves, achieve the effects of improving appearance quality, high survival rate, and increasing leaf area
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Embodiment 1
[0018] The present invention separates and screens live bacteria from the surface of fresh tobacco leaves to obtain bacterial strain P22 that promotes cell division of tobacco leaves, expands the area of tobacco leaves, and increases the yield of tobacco leaves; reduces the content of nicotine and tar, increases the aroma of tobacco leaves, reduces miscellaneous gases in tobacco leaves, and improves the quality of tobacco leaves. Bacterial strain Yj2-a; a lactic acid bacterium that improves disease resistance and stress resistance of tobacco strains (it can be a lactic acid bacterium produced by Shandong Yiyuan Kangyuan Biotechnology Co., Ltd.). Separately ferment and expand the culture to ensure that the final bacterial concentration of the culture solution of each strain is ≥ 200 million / mL. After cultivation, the compound microbial bacterial agent is obtained by aseptically mixing according to the ratio of P22:Yj2-a:rsj=1:1:2. The bacterial agent was diluted and mixed with...
Embodiment 2
[0029] Embodiment 2 The present invention promotes the use effect of the compound microbial bacterial agent of tobacco leaf growth
[0030] Test results: Test location: Tobacco Science and Technology Demonstration Park, Wuchuan County, Guizhou Province; Flue-cured tobacco variety: K326
[0031] Three treatments were set up in this experiment: (1) liquid bacterial agent (compound microbial bacterial agent for promoting the growth of tobacco leaves prepared according to the method described in the examples); (2) clean water control; (3) routine control.
[0032] The experimental design adopted a completely randomized block design with a total of 3 blocks. The planting density is 1100 plants / mu, the plant spacing is 0.5m, the row spacing is 1.2m, and one protection row is set between each treatment. Various cultivation management measures are carefully managed in accordance with local high-yield and high-quality flue-cured tobacco cultivation techniques. The tobacco leaves of e...
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