Composite microorganism bacterial agent promoting tobacco leaf growth, applications and tobacco leaf cultivation method

A technology of compound microbial bacterial agent and cultivation method, which is applied in the field of compound microbial bacterial agent and its preparation, can solve the problems of poor systematic quality of tobacco leaves, achieve the effects of improving appearance quality, high survival rate, and increasing leaf area

Active Publication Date: 2015-04-22
农博士(福建)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the above-mentioned shortcomings in the prior art that a single bacterial strain is not systematic in improving the quality of tobacco leaves, the present invention provides a composite microbial bacterial agent, which can improve the overall quality of tobacco leaves, increase the proportion of superior tobacco leaves, coordinate nutrition, and increase tobacco leaf output; Improve the disease resistance and stress resistance of tobacco plants to achieve the purpose of biological control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The present invention separates and screens live bacteria from the surface of fresh tobacco leaves to obtain bacterial strain P22 that promotes cell division of tobacco leaves, expands the area of ​​tobacco leaves, and increases the yield of tobacco leaves; reduces the content of nicotine and tar, increases the aroma of tobacco leaves, reduces miscellaneous gases in tobacco leaves, and improves the quality of tobacco leaves. Bacterial strain Yj2-a; a lactic acid bacterium that improves disease resistance and stress resistance of tobacco strains (it can be a lactic acid bacterium produced by Shandong Yiyuan Kangyuan Biotechnology Co., Ltd.). Separately ferment and expand the culture to ensure that the final bacterial concentration of the culture solution of each strain is ≥ 200 million / mL. After cultivation, the compound microbial bacterial agent is obtained by aseptically mixing according to the ratio of P22:Yj2-a:rsj=1:1:2. The bacterial agent was diluted and mixed with...

Embodiment 2

[0029] Embodiment 2 The present invention promotes the use effect of the compound microbial bacterial agent of tobacco leaf growth

[0030] Test results: Test location: Tobacco Science and Technology Demonstration Park, Wuchuan County, Guizhou Province; Flue-cured tobacco variety: K326

[0031] Three treatments were set up in this experiment: (1) liquid bacterial agent (compound microbial bacterial agent for promoting the growth of tobacco leaves prepared according to the method described in the examples); (2) clean water control; (3) routine control.

[0032] The experimental design adopted a completely randomized block design with a total of 3 blocks. The planting density is 1100 plants / mu, the plant spacing is 0.5m, the row spacing is 1.2m, and one protection row is set between each treatment. Various cultivation management measures are carefully managed in accordance with local high-yield and high-quality flue-cured tobacco cultivation techniques. The tobacco leaves of e...

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PUM

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Abstract

The invention relates to a composite microorganism bacterial agent and a preparation method therefor, and concretely relates to a composite microorganism bacterial agent promoting tobacco leaf growth, applications in tobacco leaf growth and a tobacco leaf cultivation method. The composite microorganism bacterial agent is a solution containing a bacterial strain P22, a bacterial strain Yj2-a and lactic acid bacteria. The bacterial strain P22 is Brevundimonas diminuta preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number is CGMCC No. 4122. The bacterial strain Yj2-a is Bacillus subtilis preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number is CGMCC No.4121. The composite microorganism bacterial agent can raise the whole quality of tobacco leaves, raises the proportion of superior tobacco leaves, coordinates nutrition, raises the tobacco leaf yield, raises disease resistance and stress resistance of tobacco strains, and achieves purpose of biological control.

Description

technical field [0001] The invention relates to a composite microbial bacterial agent and a preparation method thereof, in particular to a composite microbial bacterial agent for promoting the growth of tobacco leaves and its application in tobacco leaf growth and a tobacco leaf cultivation method. Background technique [0002] At present, most of the microbial agents used in the tobacco planting stage are single strains screened on the surface of tobacco leaves, and the metabolic activities of the strains are used to change some specific technical indicators of tobacco leaves. According to the effect, there are three types: (1) increase nutrients and increase tobacco leaf yield; (2) antagonize tobacco pathogenic bacteria and improve tobacco stress resistance; (3) change the internal chemical composition of tobacco leaves and improve tobacco leaf quality. The effect of a single microorganism is limited, and often only one of the three effects can be achieved, or only a part ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P21/00A01G1/00A01G7/06
Inventor 杜秉海林金新董少英姚良同丁延芹
Owner 农博士(福建)生物技术有限公司
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