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A kind of fish collagen antibacterial film with sustained release and preparation method thereof

The technology of fish collagen and antibacterial film is applied in the field of fish collagen antibacterial film and its preparation, which can solve the problems of weak physical and chemical properties, changing the mechanical properties of collagen film, unable to function for a long time, etc., so as to achieve the effect of prolonging the antibacterial effect.

Active Publication Date: 2016-03-30
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the physical and chemical properties of the pure collagen film are relatively weak. In order to improve the properties of the film, some substances can be added to the collagen solution before the film is formed. For example, natural products with biological activity can be added to improve the antibacterial ability of the collagen film. Or antioxidant properties; or add polysaccharides or lipids to change the mechanical properties, permeability, etc. of collagen membranes as needed
However, due to the volatility of the essential oil itself and the influence of light, oxygen and other factors, it is easy to be oxidized, decomposed and destroyed, so that it cannot function for a long time.

Method used

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  • A kind of fish collagen antibacterial film with sustained release and preparation method thereof
  • A kind of fish collagen antibacterial film with sustained release and preparation method thereof

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preparation example Construction

[0022] A preparation method of fish collagen antibacterial film with sustained release comprises the following steps:

[0023] (1) Preparation of cinnamon essential oil nanoliposome co-suspension

[0024] Weigh 200mg of soybean lecithin, 100-140mg of cinnamon essential oil, 25mg of cholesterol, 1.5mg of VE and 2mg of sodium cholate and dissolve them in chloroform with a volume of 60ml, and remove the organic solvent by rotary evaporation under reduced pressure, so that the lipid forms a thin film on the wall of the flask ; Then add 60ml of phosphate buffer solution with pH = 7.0 to the flask to form a suspension, and evaporate the suspension in a water bath at 40°C under vacuum to make it evenly mixed; finally add 300ul of Tween-80 to the suspension, Probe sonication: 100W, 2s on, 2s off, sonication for 6min, the cinnamon essential oil nano-liposome co-suspension is obtained;

[0025] (2) Preparation of fish collagen solution

[0026] Put 2-3g of fish collagen in a 100ml rou...

Embodiment 1

[0030] Weigh 200 mg of soybean lecithin, 100 mg of cinnamon essential oil, 25 mg of cholesterol, 1.5 mg of VE, and 2 mg of sodium cholate and dissolve them in a chloroform solution with a volume of 60 ml, and remove the organic solvent by rotary evaporation under reduced pressure, so that the lipid forms a film on the wall of the flask; Then add 60ml of phosphate buffer solution with pH=7.0 to the flask to form a suspension, and evaporate the suspension in a water bath at 40°C under vacuum to make it evenly mixed; finally add 300ul of Tween-80 to the suspension, and Ultrasound (100W, 2s off, 2s on) in an ice-water bath probe for 6 minutes to obtain cinnamon essential oil nanoliposomes.

[0031] Put 2g of collagen in a 100ml beaker, swell with 50ml of deionized water at room temperature for 2h, add 0.5g of glycerol as a plasticizer, then stir and dissolve at 45°C for 1h, mix it evenly, and make a collagen solution.

[0032] Add 50ml of cinnamon essential oil nanoliposome co-sus...

Embodiment 2

[0034] Take soybean lecithin 200mg, cinnamon essential oil 140mg, cholesterol 25mg, VE1.5mg, sodium cholate 2mg and dissolve in the chloroform solution that volume is 60ml, decompression rotary evaporation removes organic solvent, makes lipid form film on the flask wall; Then add 60ml of phosphate buffer solution with pH=7.0 to the flask to form a suspension, and evaporate the suspension in a water bath at 40°C under vacuum to make it evenly mixed; finally add 300ul of Tween-80 to the suspension, and Ultrasound (100W, 2s off, 2s on) in an ice-water bath probe for 6 minutes to obtain cinnamon essential oil nanoliposomes.

[0035] Put 3g of collagen in a 100ml beaker, swell with 50ml of deionized water at room temperature for 2h, add 0.75g of glycerol as a plasticizer, then stir and dissolve at 45°C for 1h, mix it evenly, and make a collagen solution.

[0036] Add 50ml of cinnamon essential oil nanoliposome co-suspension into 50ml of gelatin solution, stir evenly, ultrasonically...

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Abstract

The invention discloses a fish collagen antibacterial film with sustained release and a preparation method thereof, belonging to the technical field of nano liposome sustained release and antibacterial application. Dissolve fish collagen and glycerin in water to make fish collagen solution, then add cinnamon essential oil nano-liposome co-suspension, stir evenly, ultrasonically degas, pipette and spread on a plexiglass plate to dry to form a film. Compared with the simple fish collagen film, the antibacterial film made by the present invention has greatly improved antibacterial performance, and due to the protective effect of liposome on cinnamon essential oil and the slow release of cinnamon essential oil, the antibacterial performance of the composite film is sustainable longer time. In addition, the film can be biodegraded and will not pollute the environment during use. It has the functions of preventing the loss of flavor substances of food, preventing food from being polluted and oxidized, and achieving the effect of food preservation and shelf life extension.

Description

technical field [0001] The invention belongs to the technical field of nano liposome sustained release and antibacterial application, and in particular relates to a fish collagen antibacterial membrane with sustained release and a preparation method thereof. Background technique [0002] Collagen is an important natural polymer material with low antigenicity, good biocompatibility and biodegradability. Therefore, as a natural biomass resource, it is widely used in food, biomedicine, cosmetics and other industries. growing in importance and economic status. However, the physical and chemical properties of the pure collagen film are relatively weak. In order to improve the properties of the film, some substances can be added to the collagen solution before the film is formed. For example, natural products with biological activity can be added to improve the antibacterial ability of the collagen film. Or antioxidant properties; or add polysaccharides or lipids to change the me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08L91/00C08K5/053C08J5/18A01P1/00
Inventor 伍久林张其清葛尚英刘辉王霜陈立
Owner FUZHOU UNIV