Application of trehalose in preservation of anaerobic ammonia oxidation granular sludge

A technology of anaerobic ammonium oxidation and granular sludge, which is applied in anaerobic digestion treatment, sludge treatment, water/sludge/sewage treatment, etc., to achieve the effects of simple storage system, stable storage environment, and broadened application fields

Active Publication Date: 2014-05-14
HANGZHOU NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

The present invention attempts to add trehalose to preserve anammox bacteria, so as to eliminate the adverse effects of existing common preservation methods

Method used

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Embodiment 1

[0016] The composition of the inorganic salt buffer is as follows: KH 2 PO 4 10mg·L -1 , CaCl 2 2H 2 O7.4mg·L -1 , MgSO 4 2H 2 O300mg·L -1 , KHCO 3 1250mg·L -1 , the solvent is water.

[0017] The effluent sludge from the anammox reactor was collected, washed 3 times with the above-mentioned inorganic salt buffer solution, and stored in units of 0.5 L of sludge. As the storage device, choose a custom-made organic plastic bottle with a small mouth of 1.5L, and soak it in distilled water for 3 days. After the preparation work is completed, add 0.5L of distilled water and 20g of food-grade trehalose to the storage container, then transfer 0.5L of the granular sludge to be stored into an airtight container, aerate with argon for 30min, and close the lid tightly to form an airtight container. Finally, store the airtight container in a -40°C refrigerator.

[0018] After two months of storage, take out the airtight container and perform temperature adaptation before activ...

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Abstract

The invention provides application of trehalose in preservation of anaerobic ammonia oxidation granular sludge, and a method for preserving the anaerobic ammonia oxidation granular sludge. The application comprises the steps of collecting anaerobic ammonia oxidation granular sludge, and cleaning the anaerobic ammonia oxidation granular sludge for 3-5 times through an inorganic salt buffer solution; adding water and trehalose in a sealed container, wherein volume dosage of the water is 0.8-1.2 times as much as volume of the sludge and addition of the trehalose is 1-5% of total mass; transferring the cleaned sludge into the sealed container, aerating in argon atmosphere for 20-30min, sealing the container so that the sealed container is under an anaerobic condition, and preserving at minus 20-60 DEG C. The application disclosed by the invention has the main beneficial effects of (1) widening application field of the trehalose; (2) offering a new anaerobic ammonia oxidation granular sludge preservation method; and (3) providing a simple preservation system and a stable preservation environment, thus being convenient and environment-friendly.

Description

(1) Technical field [0001] The invention relates to the application of trehalose in the preservation of anaerobic ammonium oxidation granular sludge and a method for preserving the anaerobic ammonium oxidation granular sludge. (2) Background technology [0002] As a new biological denitrification process, anaerobic ammonium oxidation has been favored by many scholars for its high efficiency and energy saving. The anammox bacteria that play an important role in this process have aroused the interest of scholars, but the doubling time of anammox bacteria is long and the growth is slow, and a large number of anammox bacteria are needed in the process of treating actual wastewater. Therefore, rapid enrichment and effective preservation of anammox bacteria is the key to break through the bottleneck of application. [0003] Common preservation methods for strains include liquid paraffin method, liquid nitrogen method, freeze-drying method and gel embedding method. Although these...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/00C02F3/28
Inventor 金仁村姬玉欣彭亭瑜叶小青诸美红
Owner HANGZHOU NORMAL UNIVERSITY
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