An adaptor (45) for converting to a push-to-connect tube fitting / port a standard tube fitting / port body (10, 110, 210, 310, 410, 510, 610) having a threaded portion (11) for threaded attachment of a nut / plug (12), comprises a coupling nut / plug (12, 112, 212, 312, 412, 512, 612) having an axially inner threaded end portion for threaded attachment to the threaded portion (11) of the fitting / port body (10); a tube sealing member (15, 115, 215, 315, 415, 515, 615) contained within the nut / plug (12) for sealing to a tube (19) having a locking surface (22) spaced from the end of the tube (19); and a retention device (23) retained in the nut / plug (12), which nut / plug (12) has at an axially outer end thereof a central opening through which the tube (19) can be inserted into the coupling nut / plug (12), whereby the locking surface (22) can be engaged by the retention device (23) to prevent axial withdrawal of the tube (19) from the coupling nut / plug (12) once inserted; wherein sealing and retention of the tube (19) is effected within the confines of the coupling nut / plug (12) and separate from and without requiring modification of the standard tube fitting / port body (10).