Device and method for detecting grain size of phytoplankton in seawater

A detection device, phytoplankton technology, applied in the direction of measuring devices, individual particle analysis, particle and sedimentation analysis, etc., can solve the problems of continuous power supply, high price, time-consuming and labor-intensive, etc., to overcome bulky, improve accuracy, low cost effect

Inactive Publication Date: 2014-05-28
DALIAN MARITIME UNIVERSITY
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Problems solved by technology

Currently, flow cytometry measurement on the market must have a stable liquid flow system to ensure a constant flow rate. The instrument is large and requires continuous power supply when in use. The data processing software is also very complicated. Generally, it can only be measured in the laboratory.
[0008] In summary, the current method for measuring the particle size of marine phytoplankton has problems such as inabil

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  • Device and method for detecting grain size of phytoplankton in seawater
  • Device and method for detecting grain size of phytoplankton in seawater
  • Device and method for detecting grain size of phytoplankton in seawater

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with accompanying drawings and examples.

[0036] figure 1 shows the overall structure block diagram of the present invention, by figure 1 It can be seen that the present invention consists of a microfluidic chip 1, an LED light source 2, an optical filter A3, an optical filter B4, a photodiode 5, an automatic sampling assembly 20, an underwater environment information collection assembly 6, a Coulter counting assembly 7, a data The processing component 8 and the data transmission and display component 9 are composed. Because the LED light source 2 is an LED blue light source, the center wavelength of the filter A3 is 480nm, and the bandwidth is 30nm, because the fluorescence emitted by the excitation light source 2 to excite the microalgae chlorophyll is green light, so the center wavelength of the filter B4 is 530nm, The bandwidth is 40nm. The fluorescence emitted by the microalgae in the detect...

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Abstract

The invention discloses a device and a method for detecting the grain size of phytoplankton in seawater, wherein the device comprises a micro-fluidic chip bearing platform, a micro-fluidic chip, an excitation source component, a light detection component, a Coulter counting component, a data processing component, an underwater environment information collecting component, an automatic sampling component and a data transmitting and displaying component, wherein the micro-fluidic chip comprises a glass slide, a liquid storage hole A, a liquid storage hole B, a liquid storage hole C, a liquid storage hole D, a main channel, a detection channel, an impedance pulse sensing upstream detection channel and an impedance pulse sensing downstream detection channel. According to the invention, because the micro-fluidic chip acts as a detection micro device of grain size spectrum of phytoplankton, relevant photoelectric detection components and data processing components are small in volume, and the detection device internally contains a set of complete detection system including an automatic sampling component, a data transmitting and displaying component and the like, the device has the advantages of convenience in carrying, low cost, capability of detecting the grain size of underwater phytoplankton in the field in real time and the like.

Description

technical field [0001] The invention relates to a detection technology for particle size spectrum of phytoplankton in seawater, in particular to a particle size detection device and method for phytoplankton in seawater. Background technique [0002] The marine ecological environment is the basic condition for the survival and development of marine organisms. Any change in the ecological environment may lead to changes in the ecosystem and biological resources. Phytoplankton in the ocean is the most important primary producer and energy converter in the marine ecosystem, and plays an important role in the marine ecosystem. Changes in the marine ecological environment will lead to changes in the community structure of phytoplankton. Phytoplankton with different particle sizes contribute differently to ocean primary productivity. Thus directly affecting the material circulation and energy flow of the marine ecosystem. The species and biomass of phytoplankton determine the co...

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Application Information

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IPC IPC(8): G01N15/12
Inventor 王俊生宋有楠潘新祥孙野青李冬青
Owner DALIAN MARITIME UNIVERSITY
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