Cross pollination method for dendrobium officinale

A technology for hybrid pollination and Dendrobium officinale, which is applied in the field of hybrid breeding of rare medicinal plants, can solve the problems of high empty fruit rate, high labor intensity and high operational technical requirements, and achieves uniform and full fruit, high application value and operability. strong effect

Active Publication Date: 2014-07-02
ZHEJIANG OUING AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acquisition of Dendrobium seed embryos has become one of the factors for the industrialization of Dendrobium officinale. At present, Dendrobium seed embryos are mainly obtained through traditional artificial pollination. Disadvantages such as low production efficiency, low fruit setting rate and high empty fruit rate
As disclosed on September 23, 2009, the Chinese invention patent application "Method for ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Cut the flowers of Dendrobium officinale at the flowering period of 4 days, disperse them evenly into a clean white porcelain dish, place them in a shaker at 25°C and 180r / min for 30 minutes, and collect the pollen grains and Dendrobium officinale in the white porcelain dish Flower; after the collected Dendrobium candidum flower is cleaned and dried, put into the smoothie machine and stir for 3min, then obtain the filtrate through a 160 mesh screen, add deionized water with 1.5 times the volume of the filtrate to make the Dendrobium candidum flower in the filtrate juice; then the pollen grains obtained were added to the flower juice of Dendrobium officinale, placed in an ultrasonic cleaner for ultrasonic treatment for 30s, the ultrasonic frequency was 20KHz, and the ultrasonic power was 200W. Based on 1L of material, ultrasonic homogenization was used to obtain pollen grains of Dendrobium candidum Group suspension; then use a rubber dropper to pollinate the ...

Embodiment 2

[0019] Example 2: Cut the flowers of Dendrobium officinale at the flowering period of 7 days, disperse them evenly in a clean white porcelain dish, place them in a shaker at 27°C and 230r / min for 60 minutes, and collect the pollen grains and Dendrobium officinale in the white porcelain dish Flower; after the collected Dendrobium candidum flower is cleaned and dried, put into the smoothie machine and stir for 3min, then obtain the filtrate through a 120 mesh screen, add deionized water with 0.5 times the volume of the filtrate to make the Dendrobium candidum flower in the filtrate juice; then the pollen grains obtained are added to the flower juice of Dendrobium candidum, placed in an ultrasonic homogenizer for ultrasonic treatment for 45s, the ultrasonic frequency is 30KHz, and the ultrasonic power is 300W. Based on 1L of material, the pollen grains of Dendrobium candidum are suspended Afterwards, use the glue head dropper to pollinate the Dendrobium candidum flowers in full ...

Embodiment 3

[0020] Example 3: Take the flowers of Dendrobium officinale at the flowering stage of 5 days, disperse them evenly in a clean white porcelain dish, place them in a shaker at 26°C and 200r / min for 45 minutes, and collect the pollen grains and flowers of Dendrobium officinale in the white porcelain dish After the collected Dendrobium candidum flower is cleaned and dried, put into the smoothie machine and stir for 2.5min, then obtain the filtrate through a 140 mesh screen, add deionized water with 1 times the volume of the filtrate to make the Dendrobium candidum flower in the filtrate juice; then the pollen grains obtained were added to the flower juice of Dendrobium candidum, placed in an ultrasonic cleaner for ultrasonic treatment for 40s, ultrasonic frequency 40KHz, ultrasonic power 400W, based on 1L of material, the suspension of pollen grains of Dendrobium candidum was obtained ; Afterwards, use a rubber dropper to pollinate the suspension of Dendrobium candidum pollen gra...

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PUM

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Abstract

The invention relates to a simple and efficient cross pollination method for dendrobium officinale. The method comprises the following steps: (1) collecting pollen; (2) preparing dendrobium officinale flower juice; (3) preparing a pollen suspension; (4) pollinating; and (5) performing management at the later stage of fruit setting. A dendrobium officinale pollen load suspension is obtained by performing ultrasonic homogenization on pollen grains and the dendrobium officinale flower juice, and dendrobium officinale flowers are pollinated by the dendrobium officinale pollen load suspension by using a rubber-head burette, so that damages to stigmas of female parents are avoided, the fruit setting rate is high (over 98.2 percent), inane fruits are prevented, mature dendrobium officinale capsules can be obtained by sitting for 120 days, and fruits are uniform and plump. The method has the characteristics of easiness, convenience, high efficiency and high operability, the pollinating efficiency is 5-10 times that of a conventional pollinating method, the pollinating efficiency of dendrobium officinale is increased greatly, the application value is extremely high, and a novel high-speed and effective way is provided for hand pollination of dendrobium officinale.

Description

technical field [0001] The invention relates to a hybrid breeding method of rare medicinal plants, in particular to a hybrid pollination method of dendrobium officinale. Background technique [0002] Dendrobium of ficinale (Demdrobium of ficinale) is a perennial herbaceous plant of the genus Dendrobium orchidaceae. It is a traditional precious and rare Chinese medicinal material. At present, Dendrobium officinale has been listed as a national key protected medicinal plant and a second-class protected plant in the world. [0003] Due to the special physiological structure of Dendrobium officinale flowers, the fruiting rate of Dendrobium officinale is extremely low under natural conditions. Generally, Dendrobium officinale is in full bloom in May, and the flowering period is 10-15 days. Its fruit is a capsule, which is easy to crack when mature, and the seeds float with the wind. Scattered, the seed coat of these tiny seeds only contains immature embryos, without endosperm, s...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 汤李琼金九洲李振达
Owner ZHEJIANG OUING AGRI DEV CO LTD
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